1999
Control of CFTR Channel Gating by Phosphorylation and Nucleotide Hydrolysis
GADSBY D, NAIRN A. Control of CFTR Channel Gating by Phosphorylation and Nucleotide Hydrolysis. Physiological Reviews 1999, 79: s77-s107. PMID: 9922377, DOI: 10.1152/physrev.1999.79.1.s77.Peer-Reviewed Original ResearchConceptsNucleotide-binding domainCFTR channelsCytoplasmic nucleotide-binding domainsNucleotide hydrolysisChannel gatingDependent phosphorylation eventsCystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channelCFTR channel currentsCFTR channel gatingATP moleculesLarge cytoplasmic domainCommon lethal genetic diseaseSecond ATP moleculeSingle CFTR channelsATP hydrolysis cycleLethal genetic diseasePhosphorylation eventsGating cycleRegulatory domainCytoplasmic domainDifferent phosphoformsProgressive phosphorylationMultiple proteinsProtein productsHydrolysis cycle
1998
ATP hydrolysis cycles and the gating of CFTR Cl- channels.
Gadsby D, Dousmanis A, Nairn A. ATP hydrolysis cycles and the gating of CFTR Cl- channels. Acta Physiologica Scandinavica. Supplementum 1998, 643: 247-56. PMID: 9789567.Peer-Reviewed Original ResearchConceptsC-terminal nucleotideCFTR channelsAMP-PNPG proteinsN-terminal nucleotideCentral regulatory domainMore serine residuesProtein kinase ACFTR Cl- channelHydrolysis of ATPATP hydrolysis cycleCl- channelsGating cycleRegulatory domainCytoplasmic domainTight bindingSerine residuesHydrolyse ATPSecond ATPSequence homologyTransport proteinsKinase AOpen conformationAnalogues of ATPFunctional similarity
1994
Coupling of CFTR Cl− channel gating to an ATP hydrolysis cycle
Baukrowitz T, Hwang T, Nairn A, Gadsby D. Coupling of CFTR Cl− channel gating to an ATP hydrolysis cycle. Neuron 1994, 12: 473-482. PMID: 7512348, DOI: 10.1016/0896-6273(94)90206-2.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorATP hydrolysis cycleHydrolysis cycleCFTR channelsFibrosis transmembrane conductance regulatorProtein kinase ATransmembrane conductance regulatorATP hydrolysisKinase AConductance regulatorNucleoside triphosphatesChannel openingInorganic phosphate analogueATPPhosphate analogueCardiac myocytesInorganic phosphateMean open timeRegulatorHydrolysis productsBeF3Open timeCycleTriphosphate