2024
Photosystem II: light‐dependent oscillation of ligand composition at its active site
Wang J. Photosystem II: light‐dependent oscillation of ligand composition at its active site. Acta Crystallographica Section D, Structural Biology 2024, 80: 850-861. PMID: 39607822, DOI: 10.1107/s2059798324011392.Peer-Reviewed Original ResearchMeSH KeywordsCatalytic DomainCrystallography, X-RayLigandsLightModels, MolecularOxygenPhotosystem II Protein ComplexThermosynechococcusComposition Heterogeneity of Metal Ions Bound at the Oxygen-Evolving Center of Photosystem II in Living Cells
Wang J. Composition Heterogeneity of Metal Ions Bound at the Oxygen-Evolving Center of Photosystem II in Living Cells. Biochemistry 2024, 63: 1963-1968. PMID: 39037205, DOI: 10.1021/acs.biochem.4c00261.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCryoelectron MicroscopyMetalsModels, MolecularOxygenPhotosystem II Protein ComplexThermosynechococcusConceptsX-ray free-electron lasersStructure of photosystem IIOxygen-evolving centerPhotosystem IICrystal structure of photosystem IIFree-electron laserPhotosystem II dimersPhotosystem ILight-harvesting complexesCryo-electron tomographyMetal ionsPSII samplesCryo-electron microscopyCryo-ETDimeric core complexesCryo-EMMetal ion cofactorsLiving cellsCrystal structureCryo-electronCryo-EM structureMetal-ion occupancyIon occupancySpectroscopic interpretationAsymmetric environment
2023
Computing the Relative Affinity of Chlorophylls a and b to Light-Harvesting Complex II
Ranepura G, Mao J, Vermaas J, Wang J, Gisriel C, Wei R, Ortiz-Soto J, Uddin R, Amin M, Brudvig G, Gunner M. Computing the Relative Affinity of Chlorophylls a and b to Light-Harvesting Complex II. The Journal Of Physical Chemistry B 2023, 127: 10974-10986. PMID: 38097367, DOI: 10.1021/acs.jpcb.3c06273.Peer-Reviewed Original Research
2022
Glycerol binding at the narrow channel of photosystem II stabilizes the low-spin S2 state of the oxygen-evolving complex
Flesher DA, Liu J, Wiwczar JM, Reiss K, Yang KR, Wang J, Askerka M, Gisriel CJ, Batista VS, Brudvig GW. Glycerol binding at the narrow channel of photosystem II stabilizes the low-spin S2 state of the oxygen-evolving complex. Photosynthesis Research 2022, 152: 167-175. PMID: 35322325, PMCID: PMC9427693, DOI: 10.1007/s11120-022-00911-0.Peer-Reviewed Original ResearchMeSH KeywordsCyanobacteriaElectron Spin Resonance SpectroscopyGlycerolHydrogenOxidation-ReductionOxygenPhotosystem II Protein ComplexWaterConceptsOxygen-evolving complexHydrogen bond networkS2 stateEPR signalPhotosystem II cyclesX-ray crystal structureRelative stabilityState EPR signalsD1-Asp61Water oxidationCatalytic intermediatesPhotochemical oxidationEPR spectraGlycerol moleculesCrystal structureCyanobacterial PSIIMultiline signalState SiPhotosystem IIOxidationRelative intensitiesComplexesEffect of glycerolExperimental conditionsStability
2021
High-resolution cryo-electron microscopy structure of photosystem II from the mesophilic cyanobacterium, Synechocystis sp. PCC 6803
Gisriel CJ, Wang J, Liu J, Flesher DA, Reiss KM, Huang HL, Yang KR, Armstrong WH, Gunner MR, Batista VS, Debus RJ, Brudvig GW. High-resolution cryo-electron microscopy structure of photosystem II from the mesophilic cyanobacterium, Synechocystis sp. PCC 6803. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 119: e2116765118. PMID: 34937700, PMCID: PMC8740770, DOI: 10.1073/pnas.2116765118.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCryoelectron MicroscopyPhotosystem II Protein ComplexProtein ConformationSynechocystisConceptsCryo-electron microscopy structurePCC 6803Photosystem IIWater oxidationMicroscopy structureMesophilic cyanobacteriumHigh-resolution cryo-electron microscopy structuresOxygen-evolving photosystem IILight-driven water oxidationCyanobacterial photosystem IIHigh-resolution structuresD1 subunitPSII structureSynechocystis spLarge water channelsGenetic manipulationC-terminusBiophysical dataActive siteCyanobacteriumSpStructural pictureSubunitsOxidationWater channelsStructure of a monomeric photosystem II core complex from a cyanobacterium acclimated to far-red light reveals the functions of chlorophylls d and f
Gisriel CJ, Shen G, Ho MY, Kurashov V, Flesher DA, Wang J, Armstrong WH, Golbeck JH, Gunner MR, Vinyard DJ, Debus RJ, Brudvig GW, Bryant DA. Structure of a monomeric photosystem II core complex from a cyanobacterium acclimated to far-red light reveals the functions of chlorophylls d and f. Journal Of Biological Chemistry 2021, 298: 101424. PMID: 34801554, PMCID: PMC8689208, DOI: 10.1016/j.jbc.2021.101424.Peer-Reviewed Original ResearchMeSH KeywordsChlorophyllLightPhotosynthesisPhotosystem I Protein ComplexPhotosystem II Protein ComplexSynechococcusWaterConceptsChl f moleculesWater oxidationF moleculesPhotosystem II core complexII core complexesPhotosystem IIÅ resolution cryo-EM structureFar-red light photoacclimationResolution cryo-EM structurePhotochemical catalysisElectron transfer chainCryo-EM structureGlutamate side chainVisible lightCore complexSide chainsRed limitD moleculesSolar energy utilizationAcceptor sidePSII biogenesisFar-red lightPCC 7335Core subunitsMoleculesHeterogeneous Composition of Oxygen-Evolving Complexes in Crystal Structures of Dark-Adapted Photosystem II
Wang J, Gisriel CJ, Reiss K, Huang HL, Armstrong WH, Brudvig GW, Batista VS. Heterogeneous Composition of Oxygen-Evolving Complexes in Crystal Structures of Dark-Adapted Photosystem II. Biochemistry 2021, 60: 3374-3384. PMID: 34714055, DOI: 10.1021/acs.biochem.1c00611.Peer-Reviewed Original ResearchMeSH KeywordsChlorophyllCrystallographyElectron Spin Resonance SpectroscopyLight-Harvesting Protein ComplexesModels, TheoreticalOxidation-ReductionOxygenPhotosystem II Protein ComplexPlant ProteinsThylakoidsWaterConceptsOxygen-evolving complexMetal ionsPhotosystem IIElectron density peakIndividual metal ionsElectron density distributionNumber of electronsPSII dimersMetal centerWater oxidationOxidation stateElectron numberHomodimeric protein complexElectronsCrystal structurePSII structureDensity distributionDensity peaksComplexesRedox stateIonsDimersMonomersPeakOxidationDo crystallographic XFEL data support binding of a water molecule to the oxygen-evolving complex of photosystem II exposed to two flashes of light?
Wang J, Armstrong WH, Batista VS. Do crystallographic XFEL data support binding of a water molecule to the oxygen-evolving complex of photosystem II exposed to two flashes of light? Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2023982118. PMID: 34117119, PMCID: PMC8214663, DOI: 10.1073/pnas.2023982118.Peer-Reviewed Original Research
2019
Thermodynamics of the S 2 -to-S 3 state transition of the oxygen-evolving complex of photosystem II
Amin M, Kaur D, Yang KR, Wang J, Mohamed Z, Brudvig GW, Gunner MR, Batista V. Thermodynamics of the S 2 -to-S 3 state transition of the oxygen-evolving complex of photosystem II. Physical Chemistry Chemical Physics 2019, 21: 20840-20848. PMID: 31517382, DOI: 10.1039/c9cp02308a.Peer-Reviewed Original ResearchConceptsOxygen-evolving complexD1-E189Water/hydroxide moleculeOxygen evolution reactionWater/hydroxidePhotosystem IIVan der WaalsRedox isomersAdditional hydroxideHydroxide moleculeCatalytic intermediatesElectrostatic interactionsProton lossCoordination shellDer WaalsSerial femtosecond crystallographyHydroxide additionHydroxideConformational changesFree electron laser measurementXFEL structureAdditional oxygenMn4IntermediatesFemtosecond crystallography
2018
Reduced Occupancy of the Oxygen-Evolving Complex of Photosystem II Detected in Cryo-Electron Microscopy Maps
Wang J, Reiss K, Brudvig GW, Batista VS. Reduced Occupancy of the Oxygen-Evolving Complex of Photosystem II Detected in Cryo-Electron Microscopy Maps. Biochemistry 2018, 57: 5925-5929. PMID: 30260634, DOI: 10.1021/acs.biochem.8b00609.Peer-Reviewed Original ResearchConceptsOxygen-evolving complexElectrostatic potentialOEC of PSIIPhotosystem IIAtomic scattering factorsElectron scatteringScattering factorsDensity functional theoryESP mapsFunctional theoryAtomic coordinatesAtomistic modelMicroscopy mapsScatteringCryo-electron microscopy mapComputational simulations
2017
Determination of chemical identity and occupancy from experimental density maps
Wang J. Determination of chemical identity and occupancy from experimental density maps. Protein Science 2017, 27: 411-420. PMID: 29027293, PMCID: PMC5775170, DOI: 10.1002/pro.3325.Peer-Reviewed Original ResearchMeSH KeywordsCryoelectron MicroscopyCrystallography, X-RayFourier AnalysisMacromolecular SubstancesModels, MolecularPhotosystem II Protein ComplexProtein BindingProtein Conformation, alpha-HelicalStatic ElectricityConceptsCharge densityFourier transformElectrostatic potentialExperimental charge densitySolvent moleculesAtomic B-factorsElectron densityBasic electronic propertiesESP mapsProtein α-helixChemical identityActive siteElectronic propertiesLarge macromolecular complexesExperimental density mapsDensity mapsMoleculesVitreous iceMacromolecular complexesΑ-helixSmall protein subunitESP valuesTransformStructure factorSupercomplexesOn the relationship between cumulative correlation coefficients and the quality of crystallographic data sets
Wang J, Brudvig GW, Batista VS, Moore PB. On the relationship between cumulative correlation coefficients and the quality of crystallographic data sets. Protein Science 2017, 26: 2410-2416. PMID: 28960580, PMCID: PMC5699489, DOI: 10.1002/pro.3314.Peer-Reviewed Original ResearchCrystallography, X-RayDatabases, FactualModels, MolecularPhotosystem II Protein ComplexSignal-To-Noise RatioChlorophyll a with a farnesyl tail in thermophilic cyanobacteria
Wiwczar JM, LaFountain AM, Wang J, Frank HA, Brudvig GW. Chlorophyll a with a farnesyl tail in thermophilic cyanobacteria. Photosynthesis Research 2017, 134: 175-182. PMID: 28741056, PMCID: PMC5832022, DOI: 10.1007/s11120-017-0425-4.Peer-Reviewed Original ResearchMeSH KeywordsChlorophyllChlorophyll ACyanobacteriaElectron TransportOxygenPhotosynthesisPhotosystem II Protein ComplexConceptsThermophilic cyanobacteriaPhotosystem IIMajor light-harvesting pigmentOxygenic photosynthetic organismsLight-harvesting pigmentsÅ crystal structurePhotosynthetic organismsFarnesyl tailCyanobacteriaIsoprene unitsSpecific ChlSmall populationPhytyl tailElectron density mapsTailChlMass spectrometryOrganismsCofactorDensity mapsCrystal structureHigh-performance liquid chromatographyExperimental evidencePigmentsLiquid chromatography
2016
X‐ray radiation‐induced addition of oxygen atoms to protein residues
Wang J. X‐ray radiation‐induced addition of oxygen atoms to protein residues. Protein Science 2016, 25: 1407-1419. PMID: 27074249, PMCID: PMC4989999, DOI: 10.1002/pro.2934.Peer-Reviewed Original ResearchDestruction‐and‐diffraction by X‐ray free‐electron laser
Wang J. Destruction‐and‐diffraction by X‐ray free‐electron laser. Protein Science 2016, 25: 1585-1592. PMID: 27262052, PMCID: PMC5338243, DOI: 10.1002/pro.2959.Peer-Reviewed Original ResearchMeSH KeywordsCytochrome-c PeroxidaseElectron Transport Complex IVElectronsLasersPhotosystem II Protein ComplexProtein DomainsScattering, RadiationConceptsX-ray free-electron lasersFree-electron laserSingle femtosecond pulseAtomic scattering factorsFemtosecond pulsesRecent theoretical studiesScattering factorsSuch pulsesElectronic processesDifferent atomsCrystallographic data collectionRadiation damageSingle pulseX-rayPulsesLaserSuch crystalsTheoretical studyCrystalsDiffractionMacromolecular crystalsFemtosecondExperimental evidenceAtomsRadiationS3 State of the O2‑Evolving Complex of Photosystem II: Insights from QM/MM, EXAFS, and Femtosecond X‑ray Diffraction
Askerka M, Wang J, Vinyard DJ, Brudvig GW, Batista VS. S3 State of the O2‑Evolving Complex of Photosystem II: Insights from QM/MM, EXAFS, and Femtosecond X‑ray Diffraction. Biochemistry 2016, 55: 981-984. PMID: 26849148, DOI: 10.1021/acs.biochem.6b00041.Peer-Reviewed Original ResearchConceptsExtended X-ray absorption fine structureFemtosecond x-ray diffractionX-ray diffractionOxygen-evolving complexS3 stateHybrid quantum mechanics/molecular mechanics (QM/MM) methodX-ray absorption fine structureQuantum mechanics/molecular mechanics methodsAbsorption fine structureQM/MMPhotosystem IIMolecular mechanics methodElectron paramagnetic resonanceWater ligandsS3 transitionAmmonia bindingParamagnetic resonanceFine structureMechanics methodDiffractionComplexesStateResonanceLigandsMn4
2015
Analysis of the Radiation-Damage-Free X‑ray Structure of Photosystem II in Light of EXAFS and QM/MM Data
Askerka M, Vinyard DJ, Wang J, Brudvig GW, Batista VS. Analysis of the Radiation-Damage-Free X‑ray Structure of Photosystem II in Light of EXAFS and QM/MM Data. Biochemistry 2015, 54: 1713-1716. PMID: 25710258, DOI: 10.1021/acs.biochem.5b00089.Peer-Reviewed Original ResearchConceptsPhotosystem II crystalsX-ray absorption fine structureExtended X-ray absorption fine structureAbsorption fine structurePhotosystem IIHigh-resolution structural modelS1 stateS0 stateOxygen-evolving complexRadiation damageX-ray diffraction studiesExtensive dark adaptationFine structureDiffraction studiesOxygen atomsManganese centersX-ray structureAtomsMM dataCrystalsStateEXAFSLightStructureResolution
2014
Structural Changes in the Oxygen-Evolving Complex of Photosystem II Induced by the S1 to S2 Transition: A Combined XRD and QM/MM Study
Askerka M, Wang J, Brudvig GW, Batista VS. Structural Changes in the Oxygen-Evolving Complex of Photosystem II Induced by the S1 to S2 Transition: A Combined XRD and QM/MM Study. Biochemistry 2014, 53: 6860-6862. PMID: 25347729, PMCID: PMC4230327, DOI: 10.1021/bi5011915.Peer-Reviewed Original Research