2022
mRNA 5′ terminal sequences drive 200-fold differences in expression through effects on synthesis, translation and decay
van den Elzen A, Watson M, Thoreen C. mRNA 5′ terminal sequences drive 200-fold differences in expression through effects on synthesis, translation and decay. PLOS Genetics 2022, 18: e1010532. PMID: 36441824, PMCID: PMC9731452, DOI: 10.1371/journal.pgen.1010532.Peer-Reviewed Original ResearchConceptsTerminal sequenceGene expressionKey post-transcriptional regulatorsTerminal oligopyrimidine motifsCore promoter motifsPost-transcriptional regulatorsPromoter motifsMRNA decayTranslation initiationRegulatory sequencesReporter mRNAEfficient transcriptionLibrary sequencesEndogenous mRNARegulatory potentialNative mRNAHuman cellsTranscriptionMRNAHybrid sequencesSequenceExpressionMotifMRNA expressionTranslation
2020
Induced pluripotent stem cell reprogramming‐associated methylation at the GABRA2 promoter and chr4p12 GABAA subunit gene expression in the context of alcohol use disorder
Goetjen A, Watson M, Lieberman R, Clinton K, Kranzler H, Covault J. Induced pluripotent stem cell reprogramming‐associated methylation at the GABRA2 promoter and chr4p12 GABAA subunit gene expression in the context of alcohol use disorder. American Journal Of Medical Genetics Part B Neuropsychiatric Genetics 2020, 183: 464-474. PMID: 33029895, PMCID: PMC8022112, DOI: 10.1002/ajmg.b.32824.Peer-Reviewed Original ResearchConceptsGene expressionSubunit gene expressionSubunit geneNeural culturesInduced pluripotent stem cellsReceptor subunit genesPluripotent stem cellsStochastic methylationGene clusterDNA methylationCpG methylationMethylation levelsReceptor subunit gene expressionMethylationSignificant genetic contributionGenesStem cellsGenetic contributionMolecular effectsIPSC linesGABRB1 geneExpressionGABRA2GenotypesAdditive effect
2013
Six Innexins Contribute to Electrical Coupling of C. elegans Body-Wall Muscle
Liu P, Chen B, Altun Z, Gross M, Shan A, Schuman B, Hall D, Wang Z. Six Innexins Contribute to Electrical Coupling of C. elegans Body-Wall Muscle. PLOS ONE 2013, 8: e76877. PMID: 24130800, PMCID: PMC3793928, DOI: 10.1371/journal.pone.0076877.Peer-Reviewed Original ResearchConceptsUNC-9Body wall musclesC. elegans body wall muscleBody wall muscle cellsGap junctionsEpitope-tagged proteinsInnexinsTriple mutantPunctate localizationFunctions cellMutantsIntercellular junctionsDistinct populationsMuscle expressionGFPMuscle cellsJunctional currentsElectrical couplingFunctional propertiesCellsPromoterProteinSolid foundationExpressionPopulation