2015
Electrical behaviour of dendritic spines as revealed by voltage imaging
Popovic MA, Carnevale N, Rozsa B, Zecevic D. Electrical behaviour of dendritic spines as revealed by voltage imaging. Nature Communications 2015, 6: 8436. PMID: 26436431, PMCID: PMC4594633, DOI: 10.1038/ncomms9436.Peer-Reviewed Original ResearchCombining Membrane Potential Imaging with Other Optical Techniques
Jaafari N, Vogt KE, Saggau P, Leslie LM, Zecevic D, Canepari M. Combining Membrane Potential Imaging with Other Optical Techniques. Advances In Experimental Medicine And Biology 2015, 859: 103-125. PMID: 26238050, PMCID: PMC5675139, DOI: 10.1007/978-3-319-17641-3_4.Peer-Reviewed Original ResearchConceptsMembrane potential imagingOptical techniquesPotential imagingUncaging techniquesChannelrhodopsin stimulationVoltage-sensitive dyeVariety of applicationsSpatial mappingElectrical signalsImportant applicationsVoltageFluorescence intensityImagingParticular moleculeSignalsMembrane potential changesGateNovel investigationOptogeneticsMeasurementsIntensityTechniqueCurrent
2012
Voltage-sensitive dye recording from axons, dendrites and dendritic spines of individual neurons in brain slices.
Popovic M, Gao X, Zecevic D. Voltage-sensitive dye recording from axons, dendrites and dendritic spines of individual neurons in brain slices. Journal Of Visualized Experiments 2012, e4261. PMID: 23222505, PMCID: PMC3565854, DOI: 10.3791/4261.Peer-Reviewed Original ResearchConceptsLaser light sourceSub-micrometer resolutionLight sourceOptical recordingDetectable pharmacological effectsCurrent sensitivityVoltage-sensitive dyeSubthreshold eventsElectrical propertiesDetailed measurementsMultiple-site optical recordingVoltage-sensitive dye recordingFluorescence signalOptimal wavelengthsMembrane potential transientsElectrical signalsIndividual dendritic spinesExperimental evidenceWavelengthMeasurement oneMeasurementsBroadeningDye loadingPotential transientsBrightness
2011
Somatic Membrane Potential and Kv1 Channels Control Spike Repolarization in Cortical Axon Collaterals and Presynaptic Boutons
Foust AJ, Yu Y, Popovic M, Zecevic D, McCormick DA. Somatic Membrane Potential and Kv1 Channels Control Spike Repolarization in Cortical Axon Collaterals and Presynaptic Boutons. Journal Of Neuroscience 2011, 31: 15490-15498. PMID: 22031895, PMCID: PMC3225031, DOI: 10.1523/jneurosci.2752-11.2011.Peer-Reviewed Original ResearchMeSH Keywords4-AminopyridineAnimalsAxonsBiophysical PhenomenaComputer SimulationCrystallinsDose-Response Relationship, DrugElapid VenomsElectric StimulationFemaleGreen Fluorescent ProteinsIn Vitro TechniquesMaleMembrane PotentialsMiceMice, TransgenicModels, NeurologicalMu-CrystallinsNerve NetNeuronsPatch-Clamp TechniquesPotassium Channel BlockersPresynaptic TerminalsShaker Superfamily of Potassium ChannelsSomatosensory CortexStyrenesTetraethylammoniumConceptsAxon collateralsPresynaptic boutonsKv1 channelsSpike repolarizationPresynaptic terminalsSubthreshold depolarizationAction potentialsΑ-dendrotoxinLayer 5 pyramidal cellsIntracortical axon collateralsSynaptic neurotransmitter releaseMouse brain slicesSomatic membrane potentialKv1 subunitsPyramidal cellsSynaptic transmissionBrain slicesVoltage-sensitive dyeLow dosesNeurotransmitter releaseSynaptic strengthBoutonsCollateralsRepolarizationIon channelsThe spatio‐temporal characteristics of action potential initiation in layer 5 pyramidal neurons: a voltage imaging study
Popovic MA, Foust AJ, McCormick DA, Zecevic D. The spatio‐temporal characteristics of action potential initiation in layer 5 pyramidal neurons: a voltage imaging study. The Journal Of Physiology 2011, 589: 4167-4187. PMID: 21669974, PMCID: PMC3180577, DOI: 10.1113/jphysiol.2011.209015.Peer-Reviewed Original ResearchConceptsSpike trigger zoneAction potential initiationAxon initial segmentTrigger zoneLayer 5 pyramidal neuronsPotential initiationLayer 5 pyramidal cellsMature axonsPyramidal neuronsAxonal collateralsPyramidal cellsNeuronal plasticityAction potentialsImmature axonsPotential imaging techniqueAxonsImmunocytochemical dataNeuronal computationSaltatory conductionAP propagationElectrophysiological measuresInitial segmentImaging techniquesCritical roleInitiation
2010
Action Potentials Initiate in the Axon Initial Segment and Propagate through Axon Collaterals Reliably in Cerebellar Purkinje Neurons
Foust A, Popovic M, Zecevic D, McCormick DA. Action Potentials Initiate in the Axon Initial Segment and Propagate through Axon Collaterals Reliably in Cerebellar Purkinje Neurons. Journal Of Neuroscience 2010, 30: 6891-6902. PMID: 20484631, PMCID: PMC2990270, DOI: 10.1523/jneurosci.0552-10.2010.Peer-Reviewed Original ResearchConceptsAxon initial segmentAxon collateralsAction potentialsPurkinje neuronsComplex spikesLocal axon collateralsCerebellar Purkinje neuronsInitial segmentFast action potentialsAxon branch pointsSynaptic inputsVoltage-sensitive dyeCerebellar cortexNeuronal processingSpike initiationDischarge frequencySingle trialCollateralsOutput cellsNeuronsImaging Inhibitory Synaptic Potentials Using Voltage Sensitive Dyes
Canepari M, Willadt S, Zecevic D, Vogt KE. Imaging Inhibitory Synaptic Potentials Using Voltage Sensitive Dyes. Biophysical Journal 2010, 98: 2032-2040. PMID: 20441768, PMCID: PMC2862202, DOI: 10.1016/j.bpj.2010.01.024.Peer-Reviewed Original ResearchConceptsInhibitory postsynaptic potentialsVoltage-sensitive dyeGABAergic inhibitory postsynaptic potentialsCA1 hippocampal pyramidal neuronsPatch electrodeInhibitory synaptic potentialsInhibitory synaptic transmissionHippocampal pyramidal neuronsIntracellular chloride concentrationWhole-cell configurationPyramidal neuronsApical dendritesPostsynaptic potentialsSynaptic potentialsSynaptic transmissionBrain slicesElectrode recordingsNeuronsSensitive dyeVoltage imaging techniqueDendritesMultiple sitesVoltage imagingAxonsRapid time course of action potentials in spines and remote dendrites of mouse visual cortex neurons
Holthoff K, Zecevic D, Konnerth A. Rapid time course of action potentials in spines and remote dendrites of mouse visual cortex neurons. The Journal Of Physiology 2010, 588: 1085-1096. PMID: 20156851, PMCID: PMC2852997, DOI: 10.1113/jphysiol.2009.184960.Peer-Reviewed Original ResearchConceptsRapid time courseAction potentialsDendritic spinesIndividual dendritic spinesLayer 5 pyramidal neuronsTime courseTiming-dependent synaptic plasticityDendritic branchesCentral mammalian neuronsTerminal dendritic branchesVisual cortex neuronsTerminal branchesSomatic action potentialsIndividual spinesRemote dendritesPyramidal neuronsApical dendritesCortex neuronsExcitatory synapsesSupralinear integrationSynaptic inputsVoltage-sensitive dyeJuvenile miceSynaptic plasticityVisual cortex
2005
Imaging of Spiking and Subthreshold Activity of Mitral Cells with Voltage‐Sensitive Dyes
DJURIŠIĆ M, ZEČEVIĆ D. Imaging of Spiking and Subthreshold Activity of Mitral Cells with Voltage‐Sensitive Dyes. Annals Of The New York Academy Of Sciences 2005, 1048: 92-102. PMID: 16154924, DOI: 10.1196/annals.1342.009.Peer-Reviewed Original ResearchConceptsSpike trigger zoneMitral cellsSite of originTrigger zonePrincipal output neuronsTerminal dendritic branchesPrimary dendritesOlfactory nerve stimulationRat olfactory bulbNerve stimulationDendritic tuftsOlfactory bulbVoltage-sensitive dyeAction potentialsNeuronal processesSubthreshold activityIndividual neuronsDendritic branchesSpike initiationIntracellular dyeEPSPsOutput neuronsNeuronsDendritesCellsDeterminants of Low EPSP Attenuation in Primary Dendrites of Mitral Cells: Modeling Study
POPOVIĆ M, DJURIŠIĆ M, ZEČEVIĆ D. Determinants of Low EPSP Attenuation in Primary Dendrites of Mitral Cells: Modeling Study. Annals Of The New York Academy Of Sciences 2005, 1048: 344-348. PMID: 16154948, DOI: 10.1196/annals.1342.033.Peer-Reviewed Original Research
2000
Functional profile of the giant metacerebral neuron of Helix aspersa: temporal and spatial dynamics of electrical activity in situ
Antic S, Wuskell J, Loew L, Zecevic D. Functional profile of the giant metacerebral neuron of Helix aspersa: temporal and spatial dynamics of electrical activity in situ. The Journal Of Physiology 2000, 527: 55-69. PMID: 10944170, PMCID: PMC2270048, DOI: 10.1111/j.1469-7793.2000.00055.x.Peer-Reviewed Original ResearchConceptsTrigger zoneNeuronal processesGiant metacerebral neuronSingle neuronsMulti-site optical recordingSpike trigger zoneAction potential initiationIdentified snail neuronsMetacerebral neuronsAxonal branchesVoltage-sensitive dyeSynaptic inputsPropagation of spikesSnail neuronsAction potentialsAxonal spikesPatterns of initiationNeuronsPotential initiationSynaptic signalsIndividual neuronsElectrical activityHelix aspersaFunctional profilesInitiation
1999
Fast multisite optical measurement of membrane potential: three examples
Antic S, Cohen L, Lam Y, Wachowiak M, Zecevic D, Zochowski M. Fast multisite optical measurement of membrane potential: three examples. The FASEB Journal 1999, 13: s271-s276. PMID: 10619142, DOI: 10.1096/fasebj.13.9002.s271.Peer-Reviewed Original ResearchFast Optical Recordings of Membrane Potential Changes From Dendrites of Pyramidal Neurons
Antic S, Major G, Zecevic D. Fast Optical Recordings of Membrane Potential Changes From Dendrites of Pyramidal Neurons. Journal Of Neurophysiology 1999, 82: 1615-1621. PMID: 10482775, DOI: 10.1152/jn.1999.82.3.1615.Peer-Reviewed Original ResearchConceptsBasolateral dendritesApical dendritesBrain slicesVoltage-sensitive dyeNeuronal processesPyramidal neuronsMembrane potential transientsPharmacological effectsSynaptic stimulationAction potentialsInvertebrate neuronsNeuronsPatch electrodeSingle neuronsElectrical activityMembrane potential changesFast optical recordingDistal processesVertebrate neuronsOptical recordingDendritesH incubation periodStimulationApical processesRecordings
1998
Fast optical measurement of membrane potential changes at multiple sites on an individual nerve cell
Zecevic D, Antic S. Fast optical measurement of membrane potential changes at multiple sites on an individual nerve cell. Journal Of Molecular Histology 1998, 30: 197-216. PMID: 10188927, DOI: 10.1023/a:1003299420524.Peer-Reviewed Original Research
1997
Fast voltage-sensitive dye recording of membrane potential changes at multiple sites on an individual nerve cell in the rat cortical slice.
Antić S, Major G, Chen W, Wuskel J, Loew L, Zecević D. Fast voltage-sensitive dye recording of membrane potential changes at multiple sites on an individual nerve cell in the rat cortical slice. Biological Bulletin 1997, 193: 261. PMID: 9390400, DOI: 10.1086/bblv193n2p261.Peer-Reviewed Original Research
1995
Optical recording from cerebellar Purkinje cells using intracellularly injected voltage-sensitive dyes
Kogan A, Ross W, Zecevic D, Lasser-Ross N. Optical recording from cerebellar Purkinje cells using intracellularly injected voltage-sensitive dyes. Brain Research 1995, 700: 235-239. PMID: 8624715, DOI: 10.1016/0006-8993(95)00956-q.Peer-Reviewed Original ResearchOptical signals from neurons with internally applied voltage-sensitive dyes
Antic S, Zecevic D. Optical signals from neurons with internally applied voltage-sensitive dyes. Journal Of Neuroscience 1995, 15: 1392-1405. PMID: 7869106, PMCID: PMC6577832, DOI: 10.1523/jneurosci.15-02-01392.1995.Peer-Reviewed Original ResearchConceptsOptical signalVoltage-sensitive dyeSilicon photodiode arrayAxonal branchesAction potentialsIndividual neuronsOptical monitoringPresent sensitivityPhotodiode arraySite of stimulationAction potential initiationLong axonal branchesSatisfactory signalIntracellular pressure injectionSynaptic potentialsFluorescence measurementsGood signalAxonal segmentsSubthreshold potentialsExtracellular applicationCell bodiesNeuronal processesFluorescence signalElectrotonic responsesPotential initiation
1993
Optical measurement of action potential activity in invertebrate ganglia.
Wu J, Falk C, Cohen L, Tsau Y, Zecevic D. Optical measurement of action potential activity in invertebrate ganglia. The Journal Of Physiological Sciences 1993, 43 Suppl 1: s21-9. PMID: 8271496.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
1989
Hundreds of neurons in the Aplysia abdominal ganglion are active during the gill-withdrawal reflex
Zecevic D, Wu J, Cohen L, London J, Hopp H, Falk C. Hundreds of neurons in the Aplysia abdominal ganglion are active during the gill-withdrawal reflex. Journal Of Neuroscience 1989, 9: 3681-3689. PMID: 2795148, PMCID: PMC6569909, DOI: 10.1523/jneurosci.09-10-03681.1989.Peer-Reviewed Original ResearchOptical Measurement of Action Potential Activity in Invertebrate Ganglia
Cohen L, Hopp H, Wu J, Xiao C, London J, Zecevic D. Optical Measurement of Action Potential Activity in Invertebrate Ganglia. Annual Review Of Physiology 1989, 51: 527-541. PMID: 2653195, DOI: 10.1146/annurev.ph.51.030189.002523.Peer-Reviewed Original ResearchConceptsAplysia abdominal ganglionGill withdrawal reflexBuccal gangliaAbdominal ganglionAction potential activitySynaptic potentialsGill withdrawalSpike activityGangliaNeuronal basisNeuronsInvertebrate gangliaBehaving animalsReflexNetwork of cellsPotential activityFeedingOptical measurementsActivity