2015
Combining Membrane Potential Imaging with Other Optical Techniques
Jaafari N, Vogt KE, Saggau P, Leslie LM, Zecevic D, Canepari M. Combining Membrane Potential Imaging with Other Optical Techniques. Advances In Experimental Medicine And Biology 2015, 859: 103-125. PMID: 26238050, PMCID: PMC5675139, DOI: 10.1007/978-3-319-17641-3_4.Peer-Reviewed Original ResearchConceptsMembrane potential imagingOptical techniquesPotential imagingUncaging techniquesChannelrhodopsin stimulationVoltage-sensitive dyeVariety of applicationsSpatial mappingElectrical signalsImportant applicationsVoltageFluorescence intensityImagingParticular moleculeSignalsMembrane potential changesGateNovel investigationOptogeneticsMeasurementsIntensityTechniqueCurrent
2012
Voltage-sensitive dye recording from axons, dendrites and dendritic spines of individual neurons in brain slices.
Popovic M, Gao X, Zecevic D. Voltage-sensitive dye recording from axons, dendrites and dendritic spines of individual neurons in brain slices. Journal Of Visualized Experiments 2012, e4261. PMID: 23222505, PMCID: PMC3565854, DOI: 10.3791/4261.Peer-Reviewed Original ResearchConceptsLaser light sourceSub-micrometer resolutionLight sourceOptical recordingDetectable pharmacological effectsCurrent sensitivityVoltage-sensitive dyeSubthreshold eventsElectrical propertiesDetailed measurementsMultiple-site optical recordingVoltage-sensitive dye recordingFluorescence signalOptimal wavelengthsMembrane potential transientsElectrical signalsIndividual dendritic spinesExperimental evidenceWavelengthMeasurement oneMeasurementsBroadeningDye loadingPotential transientsBrightness
2010
Combined Voltage and Calcium Imaging and Signal Calibration
Canepari M, Saggau P, Zecevic D. Combined Voltage and Calcium Imaging and Signal Calibration. 2010, 43-52. DOI: 10.1007/978-1-4419-6558-5_4.Peer-Reviewed Original ResearchOptical measurementsVoltage-sensitive dye signalsVoltage imagingAbsolute scaleCapability of voltageVoltage-sensitive dyeFluorescent voltage-sensitive dyeSignal calibrationDye signalsSpatial mappingVoltageMeasurementsVoltage signalImagingMembrane potential changesSignalsCalibrationCombined voltageFundamental problem
2009
Imaging Membrane Potential with Voltage-Sensitive Dyes
Vucinic D, Kosmidis E, Falk C, Cohen L, Loew L, Djurisic M, Zecevic D. Imaging Membrane Potential with Voltage-Sensitive Dyes. 2009, 132-160. DOI: 10.1093/oso/9780195150445.003.0006.Peer-Reviewed Original ResearchMembrane-bound dyeOptical signalSecond harmonic generationVoltage-sensitive dyeDifferent optical propertiesHarmonic generationOptical measurementsOptical propertiesRaman absorptionOptical recordingRise timeSimultaneous measurementAbsorptionFigure 6.1BirefringenceFluorescenceMeasurementsDichroismDyeMolecular probesSignalsSquidKinds of results