2016
A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices
Abdelfattah AS, Farhi SL, Zhao Y, Brinks D, Zou P, Ruangkittisakul A, Platisa J, Pieribone VA, Ballanyi K, Cohen AE, Campbell RE. A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices. Journal Of Neuroscience 2016, 36: 2458-2472. PMID: 26911693, PMCID: PMC4764664, DOI: 10.1523/jneurosci.3484-15.2016.Peer-Reviewed Original ResearchConceptsVoltage indicatorsBlue-shifted channelrhodopsinRed-shifted fluorescent proteinsFluorescent voltage indicatorsWide-field fluorescence microscopyBlue excitationOptical imagingOptical electrophysiologyLow phototoxicityAutofluorescent backgroundLight photoactivationSingle-trial recordingsTemporal resolutionChannelrhodopsinIntrinsic advantagesExcitationVoltage oscillationsFluorescence microscopyOscillationsGreen indicatorsChromophoreMicroscopyResolution
2004
The role of actin in the regulation of dendritic spine morphology and bidirectional synaptic plasticity
Chen Y, Bourne J, Pieribone VA, Fitzsimonds RM. The role of actin in the regulation of dendritic spine morphology and bidirectional synaptic plasticity. Neuroreport 2004, 15: 829-832. PMID: 15073524, DOI: 10.1097/00001756-200404090-00018.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBridged Bicyclo Compounds, HeterocyclicCytochalasin DDendritesElectric StimulationExcitatory Postsynaptic PotentialsHippocampusIn Vitro TechniquesMaleMicroscopy, ElectronNeuronal PlasticityNeuronsNucleic Acid Synthesis InhibitorsRatsRats, Sprague-DawleySynapsesThiazolesThiazolidinesConceptsExcitatory synaptic transmissionDendritic spine morphologySynaptic transmissionSpine morphologyRat hippocampal slicesHigh-frequency stimulationLong-term depressionBidirectional synaptic plasticityHippocampal slicesIrreversible depressionExcitatory synapsesDendritic spinesMammalian CNSSynaptic efficacySynaptic plasticityDepressionSpineCNSPotentiationSynapses
1998
Galanin–5-hydroxytryptamine interactions: electrophysiological, immunohistochemical and in situ hybridization studies on rat dorsal raphe neurons with a note on galanin R1 and R2 receptors
Xu Z, Zhang X, Pieribone VA, Grillner S, Hökfelt T. Galanin–5-hydroxytryptamine interactions: electrophysiological, immunohistochemical and in situ hybridization studies on rat dorsal raphe neurons with a note on galanin R1 and R2 receptors. Neuroscience 1998, 87: 79-94. PMID: 9722143, DOI: 10.1016/s0306-4522(98)00151-1.Peer-Reviewed Original ResearchConceptsDorsal raphe neuronsRaphe neuronsRat dorsal raphe neuronsCell bodiesOutward currentsInhibitory effectGalanin-like immunoreactivityDorsal raphe nucleusDose-dependent hyperpolarizationExtracellular potassium concentrationGalaninergic mechanismsSitu hybridization studiesGalanin receptorsRaphe nucleusSynaptic contactsNerve endingsPostsynaptic receptorsSoma levelGalaninImmunohistochemical analysisR2 receptorsGalanin R1NeuronsMood regulationPhysiological concentrations
1995
Galanin induces a hyperpolarization of norepinephrine-containing locus coeruleus neurons in the brainstem slice
Pieribone VA, Xu Z, Zhang X, Grillner S, Bartfai T, Hökfelt T. Galanin induces a hyperpolarization of norepinephrine-containing locus coeruleus neurons in the brainstem slice. Neuroscience 1995, 64: 861-874. PMID: 7538638, DOI: 10.1016/0306-4522(94)00450-j.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrain StemCalciumGalaninImmunohistochemistryLocus CoeruleusMaleMembrane PotentialsPeptidesRatsRats, Sprague-DawleyTetrodotoxinConceptsLocus coeruleus neuronsCoeruleus neuronsLocus coeruleusNet outward currentGalanin responseOutward currentsATP-sensitive potassium channelsNorepinephrine-containing locus coeruleus neuronsCoexistence of galaninEffects of galaninPotassium concentrationExtracellular potassium concentrationEndogenous galaninGalanin applicationNormal potassium concentrationGalanin effectsGalanin immunoreactivityBrainstem slicesNorepinephrine neuronsAxonal originLow calcium mediumNeuronal somataSlice preparationImmunohistochemical stainingGalanin
1994
Distribution of alpha 1 adrenoceptors in rat brain revealed by in situ hybridization experiments utilizing subtype-specific probes
Pieribone V, Nicholas A, Dagerlind A, Hokfelt T. Distribution of alpha 1 adrenoceptors in rat brain revealed by in situ hybridization experiments utilizing subtype-specific probes. Journal Of Neuroscience 1994, 14: 4252-4268. PMID: 8027777, PMCID: PMC6577046, DOI: 10.1523/jneurosci.14-07-04252.1994.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainIn Situ HybridizationMaleRatsRats, Sprague-DawleyReceptors, Adrenergic, alphaRNA, MessengerTissue DistributionConceptsAlpha 1A/DSpinal motor nucleiSpinal cordCerebral cortexMotor nucleusAmygdaloid nucleusLight labellingCA1-CA4 regionsAlpha-1 adrenoceptorsAlpha 1B-adrenoceptorsIntermediolateral cell columnInternal plexiform layerLateral amygdaloid nucleusReticular thalamic nucleusDistribution of neuronsMedullary raphe nucleiMedullary reticular formationCentral amygdaloid nucleusLateral hypothalamic nucleusInferior olivary nucleusReceptor distribution patternsSubtype-specific probesAdrenoceptor mRNANoradrenergic innervationAdrenoceptor subtypesA functional role for nitric oxide in locus coeruleus: immunohistochemical and electrophysiological studies
Xu Z, Pieribone V, Zhang X, Grillner S, Hökfelt T. A functional role for nitric oxide in locus coeruleus: immunohistochemical and electrophysiological studies. Experimental Brain Research 1994, 98: 75-83. PMID: 7516892, DOI: 10.1007/bf00229111.Peer-Reviewed Original ResearchMeSH Keywords6-Cyano-7-nitroquinoxaline-2,3-dioneAmino Acid OxidoreductasesAnimalsArginineElectric StimulationElectrophysiologyEvoked PotentialsHemoglobinsImmunohistochemistryIn Vitro TechniquesLocus CoeruleusMaleMicroscopy, ElectronNG-Nitroarginine Methyl EsterNitric OxideNitric Oxide SynthaseOmega-N-MethylarginineQuinoxalinesRatsRats, Sprague-DawleySynapsesSynaptic TransmissionTyrosine 3-MonooxygenaseConceptsNeuronal nitric oxideExcitatory postsynaptic potentialsLocus coeruleusNitric oxideFocal electrical stimulationNitric oxide synthaseArginine methyl esterNeuronal cell bodiesRat locus coeruleusNOS inhibitor nitroLC neuronsNG-monomethylLike immunoreactivityNOS inhibitorPostsynaptic potentialsBath applicationOxide synthaseIntracellular recordingsSynaptic transmissionBrain slicesImmunohistochemical analysisElectrophysiological studiesElectrical stimulationAxonal processesCell bodies
1993
Cellular localization of messenger RNA for beta-1 and beta-2 adrenergic receptors in rat brain: An in situ hybridization study
Nicholas AP, Pieribone VA, Hökfelt T. Cellular localization of messenger RNA for beta-1 and beta-2 adrenergic receptors in rat brain: An in situ hybridization study. Neuroscience 1993, 56: 1023-1039. PMID: 8284033, DOI: 10.1016/0306-4522(93)90148-9.Peer-Reviewed Original ResearchConceptsSpinal cordRat brainCentral nervous system circuitryBeta 1Messenger RNABeta-receptor subtypesThalamic intralaminar nucleiAnterior olfactory nucleusBeta 1 receptorsIntermediate gray matterReticular thalamic nucleusMedullary reticular formationNervous system circuitryReceptor messenger RNABeta 1 messenger RNABeta-2 adrenergic receptorsCentral nervous systemRat beta 1Selective labeling patternsDeep cerebellar nucleiUnfixed rat brainOlfactory nucleusTrapezoid nucleusCerebral cortexSitu hybridization studiesDistributions of mRNAs for alpha‐2 adrenergic receptor subtypes in rat brain: An in situ hybridization study
Nicholas A, Pieribone V, Hökfelt T. Distributions of mRNAs for alpha‐2 adrenergic receptor subtypes in rat brain: An in situ hybridization study. The Journal Of Comparative Neurology 1993, 328: 575-594. PMID: 8381444, DOI: 10.1002/cne.903280409.Peer-Reviewed Original ResearchConceptsCentral nervous systemNervous systemCerebral cortexSpinal cordReceptor subtypesRat brainAlpha-2 adrenergic receptor subtypesVentrolateral medullary reticular formationRat central nervous systemAlpha-2 receptor subtypesMRNA labelingAlpha 2 receptorsIntermediolateral cell columnThoracic spinal cordNucleus tractus solitariiReticular thalamic nucleusHypothalamic paraventricular nucleusMedullary reticular formationDorsal root gangliaIslands of CallejaAdrenergic receptor subtypesSelective labeling patternsDeep cerebellar nucleiAlpha 2 probeRat alpha-2ACGRP-like immunoreactivity in A11 dopamine neurons projecting to the spinal cord and a note on CGRP-CCK cross-reactivity
Orazzo C, Pieribone V, Ceccatelli S, Terenius L, Hökfelt T. CGRP-like immunoreactivity in A11 dopamine neurons projecting to the spinal cord and a note on CGRP-CCK cross-reactivity. Brain Research 1993, 600: 39-48. PMID: 8422589, DOI: 10.1016/0006-8993(93)90399-8.Peer-Reviewed Original ResearchConceptsCGRP antiserumSpinal cordRat brainCalcitonin gene-related peptideDopamine cell groupsCGRP-like immunoreactivityGene-related peptideIndirect immunofluorescence techniqueLike immunoreactivityDopamine neuronsBrain areasDouble-labeling procedureImmunofluorescence techniqueCell groupsCholecystokininCatecholamine groupsCordImmunohistochemistryImmunoreactivityNeuronsRadioimmunoassayBrainPresent studyTerminal portionAntiserum
1992
Galanin message-associated peptide (GMAP)- and galanin-like immunoreactivities: Overlapping and differential distributions in the rat
Hökfelt T, Åman K, Arvidsson U, Bedecs K, Ceccatelli S, Hulting A, Langel U, Meister B, Pieribone V, Bartfai T. Galanin message-associated peptide (GMAP)- and galanin-like immunoreactivities: Overlapping and differential distributions in the rat. Neuroscience Letters 1992, 142: 139-142. PMID: 1280789, DOI: 10.1016/0304-3940(92)90358-e.Peer-Reviewed Original ResearchConceptsGalanin-like immunoreactivityGalanin message-associated peptideCentral nervous systemIslets of LangerhansGalanin messageGalanin-LIMost neuronsGalanin antiserumAnterior lobeNervous systemEndocrine tissuesInsulin cellsIndirect immunofluorescenceImmunoreactivityProlactin cellsRatsNeuronsTissueCellsDifferential distributionPancreasPituitaryPeptidesRetinaBrain