2018
Fast, in vivo voltage imaging using a red fluorescent indicator
Kannan M, Vasan G, Huang C, Haziza S, Li JZ, Inan H, Schnitzer MJ, Pieribone VA. Fast, in vivo voltage imaging using a red fluorescent indicator. Nature Methods 2018, 15: 1108-1116. PMID: 30420685, PMCID: PMC6516062, DOI: 10.1038/s41592-018-0188-7.Peer-Reviewed Original ResearchConceptsOptical toolsOptical toolboxUnparalleled temporal resolutionRed fluorescent indicatorVoltage imagingOptical electrophysiologyModest illumination intensitiesHigh-throughput strategyVoltage indicatorsIllumination intensityNew hueAcute brain slicesMultispectral imagingGreen probesSubthreshold voltageTemporal resolutionEnhanced sensitivityPostsynaptic potentialsMRuby3Brain slicesHippocampal rhythmsActivity monitorFunctional imagingImagingGEVIs
2016
A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices
Abdelfattah AS, Farhi SL, Zhao Y, Brinks D, Zou P, Ruangkittisakul A, Platisa J, Pieribone VA, Ballanyi K, Cohen AE, Campbell RE. A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices. Journal Of Neuroscience 2016, 36: 2458-2472. PMID: 26911693, PMCID: PMC4764664, DOI: 10.1523/jneurosci.3484-15.2016.Peer-Reviewed Original ResearchConceptsVoltage indicatorsBlue-shifted channelrhodopsinRed-shifted fluorescent proteinsFluorescent voltage indicatorsWide-field fluorescence microscopyBlue excitationOptical imagingOptical electrophysiologyLow phototoxicityAutofluorescent backgroundLight photoactivationSingle-trial recordingsTemporal resolutionChannelrhodopsinIntrinsic advantagesExcitationVoltage oscillationsFluorescence microscopyOscillationsGreen indicatorsChromophoreMicroscopyResolution
2014
Development of a Red Genetically-Encoded Voltage Indicator and its use with Channelrhodopsin for All-Optical Electrophysiology
Abdelfattah A, Platisa J, Zhao Y, Pieribone V, Campbell R. Development of a Red Genetically-Encoded Voltage Indicator and its use with Channelrhodopsin for All-Optical Electrophysiology. Biophysical Journal 2014, 106: 629a-630a. DOI: 10.1016/j.bpj.2013.11.3482.Peer-Reviewed Original ResearchOptical electrophysiologyVoltage indicators
2013
Genetically Targeted Optical Electrophysiology in Intact Neural Circuits
Cao G, Platisa J, Pieribone VA, Raccuglia D, Kunst M, Nitabach MN. Genetically Targeted Optical Electrophysiology in Intact Neural Circuits. Cell 2013, 154: 904-913. PMID: 23932121, PMCID: PMC3874294, DOI: 10.1016/j.cell.2013.07.027.Peer-Reviewed Original ResearchConceptsIntact neural circuitsNeural circuitsIntact brain tissueMembrane potentialNeuronal information processingNervous systemAction potentialsBrain tissueNeuronsStudy of intracellularElectrical activityKey cellular parametersMultiple neuronsElectrical eventsSubthreshold eventsNeurite branchesOptical electrophysiologyReliable recordingCellular parametersVoltage indicatorsFluorescent voltage indicatorsBrain