Sudheer Kumar Cheppali
Associate Research ScientistCards
About
Research
Publications
2025
Vesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1
Tsemperouli M, Cheppali S, Rivera-Molina F, Chetrit D, Landajuela A, Toomre D, Karatekin E. Vesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1. Biophysical Journal 2025, 124: 1878. PMID: 40010339, DOI: 10.1016/j.bpj.2025.02.013.Peer-Reviewed Original ResearchDSP-1, the major fibronectin type-II protein of donkey seminal plasma is a small heat-shock protein and exhibits chaperone-like activity against thermal and oxidative stress
Alim S, Cheppali S, Pawar S, Swamy M. DSP-1, the major fibronectin type-II protein of donkey seminal plasma is a small heat-shock protein and exhibits chaperone-like activity against thermal and oxidative stress. Biochimica Et Biophysica Acta (BBA) - Proteins And Proteomics 2025, 1873: 141064. PMID: 39956303, DOI: 10.1016/j.bbapap.2025.141064.Peer-Reviewed Original ResearchConceptsChaperone-like activitySeminal plasmaFibronectin type IITetramer to monomersSperm capacitationSurface hydrophobicityMolecular chaperonesClient proteinsHeat shock proteinsBiophysical studiesAlcohol dehydrogenaseOxidative stressPhysiological ligandsShock proteinsProteinHead group moietySHspsBinding of phosphorylcholineCholine phospholipidsBindingFibronectinDehydrogenaseChaperoneSpermMammalsSingle-molecule two- and three-colour FRET studies reveal a transition state in SNARE disassembly by NSF
Cheppali S, Li C, Xing W, Sun R, Yang M, Xue Y, Lu S, Yao J, Sun S, Chen C, Sui S. Single-molecule two- and three-colour FRET studies reveal a transition state in SNARE disassembly by NSF. Nature Communications 2025, 16: 250. PMID: 39747074, PMCID: PMC11695992, DOI: 10.1038/s41467-024-55531-0.Peer-Reviewed Original ResearchConceptsN-ethylmaleimide sensitive factorSNARE complexDisassembly of SNARE complexesStable four-helix bundleFour-helix bundleSNARE motifFluorescence spectroscopy approachesMinimal machineryAAA+ ATPasesProtein machineryAdapter proteinVesicle fusionMembrane fusionSyntaxinPhysiological processesSNAREProteinN-ethylmaleimideDisassemblySequential disassemblyMachinerySequential pathwayPathwayEukaryotesFusion
2024
Vesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1
Tsemperouli M, Cheppali S, Rivera-Molina F, Chetrit D, Landajuela A, Toomre D, Karatekin E. Vesicle docking and fusion pore modulation by the neuronal calcium sensor Synaptotagmin-1. Biophysical Journal 2024, 124: 1798-1814. PMID: 39719826, DOI: 10.1016/j.bpj.2024.12.023.Peer-Reviewed Original ResearchVesicle dockingSynaptotagmin-1C2 domainFusion triggeringCalcium sensor synaptotagmin 1Neuronal SNARE complexFusion pore dynamicsSensor synaptotagmin-1Domains of Syt1Neuroendocrine cell linePolybasic patchSNARE complexTriggers exocytosisFusion poreFusion eventsDense-core vesiclesSyt1Synaptic vesiclesPlasma membranePore regulationCalcium sensorNegatively charged phospholipidsBind calciumPore dynamicsCell linesStructure, Self-Assembly, and Phase Behavior of Neuroactive N‑Acyl GABAs: Doxorubicin Encapsulation in NPGABA/DPPC Liposomes and Release Studies
Chinapaka R, Sivaramakrishna D, Choudhury S, Manasa K, Cheppali S, Swamy M. Structure, Self-Assembly, and Phase Behavior of Neuroactive N‑Acyl GABAs: Doxorubicin Encapsulation in NPGABA/DPPC Liposomes and Release Studies. Langmuir 2024, 40: 7883-7895. PMID: 38587263, DOI: 10.1021/acs.langmuir.3c03615.Peer-Reviewed Original ResearchConceptsHydrogen bondsSelf-assemblyPowder X-ray diffraction studiesX-ray diffraction studiesOdd-even alternationN-acyl amino acidsDifferential scanning calorimetric studiesPhase behaviorAmide moietyAdjacent moleculesPhysiologically relevant pH.Molecular packingDiffraction studiesStable unilamellar vesiclesCrystal structureChain compoundsDimeric structureCarboxyl groupsHomologous seriesSupramolecular organizationThermotropic phase behaviorEquimolar mixtureRelevant pH.Calorimetric studiesChain lengthMechanisms of vesicle docking and fusion pore expansion by calcium-sensor synaptotagmin-1
Cheppali S, Tsemperouli M, Chetrit D, Rivera-Molina F, Toomre D, Karatekin E. Mechanisms of vesicle docking and fusion pore expansion by calcium-sensor synaptotagmin-1. Biophysical Journal 2024, 123: 381a. DOI: 10.1016/j.bpj.2023.11.2328.Peer-Reviewed Original Research
2023
Contrasting effects of molecular crowding on the membrane-perturbing and chaperone-like activities of major bovine seminal plasma protein, PDC-109
Singh B, Cheppali S, Saha I, Swamy M. Contrasting effects of molecular crowding on the membrane-perturbing and chaperone-like activities of major bovine seminal plasma protein, PDC-109. International Journal Of Biological Macromolecules 2023, 254: 127573. PMID: 37923045, DOI: 10.1016/j.ijbiomac.2023.127573.Peer-Reviewed Original ResearchConceptsChaperone-like activityPDC-109Effects of molecular crowdingMembrane destabilizationMolecular crowdingBovine seminal plasma proteinSperm cell surfaceSeminal plasma proteinsBovine seminal plasmaAcrosome reactionClient proteinsProtein stabilityMicroenvironment of proteinsSeminal plasmaMembrane-perturbingProtein oligomersReduced surface hydrophobicityOligomeric proteinsLipid effluxCell surfaceCrowding agentsProteinIsothermal titration calorimetric studiesTitration calorimetric studiesFunctional activityPLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection
Xu D, Jiang W, Wu L, Gaudet R, Park E, Su M, Cheppali S, Cheemarla N, Kumar P, Uchil P, Grover J, Foxman E, Brown C, Stansfeld P, Bewersdorf J, Mothes W, Karatekin E, Wilen C, MacMicking J. PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection. Nature 2023, 619: 819-827. PMID: 37438530, PMCID: PMC10371867, DOI: 10.1038/s41586-023-06322-y.Peer-Reviewed Original ResearchConceptsC-terminal β-barrel domainSpike-mediated fusionCell-autonomous defenseLarge-scale exome sequencingΒ-barrel domainGenome-wide CRISPRSARS-CoV-2 infectionHost cell cytosolScramblase activityPhospholipid scramblaseLive SARS-CoV-2 infectionHuman lung epitheliumPLSCR1SARS-CoV-2 USASingle-molecule switchingSARS-CoV-2 variantsExome sequencingHuman populationRestriction factorsViral RNANew SARS-CoV-2 variantsSARS-CoV-2Robust activityLung epitheliumDefense factorsTetraspanin 4 stabilizes membrane swellings and facilitates their maturation into migrasomes
Dharan R, Huang Y, Cheppali S, Goren S, Shendrik P, Wang W, Qiao J, Kozlov M, Yu L, Sorkin R. Tetraspanin 4 stabilizes membrane swellings and facilitates their maturation into migrasomes. Nature Communications 2023, 14: 1037. PMID: 36823145, PMCID: PMC9950420, DOI: 10.1038/s41467-023-36596-9.Peer-Reviewed Original Research
2022
Supported Natural Membranes on Microspheres for Protein–Protein Interaction Studies
Cheppali S, Dharan R, Katzenelson R, Sorkin R. Supported Natural Membranes on Microspheres for Protein–Protein Interaction Studies. ACS Applied Materials & Interfaces 2022, 14: 49532-49541. PMID: 36306148, DOI: 10.1021/acsami.2c13095.Peer-Reviewed Original ResearchMembrane proteinsProtein-protein interaction studiesPlasma membraneMembrane-related biological processesProtein-membrane interaction studiesSpike proteinEukaryotic plasma membraneProtein of SARS-CoV-2Cell-cell communicationSpike protein of SARS-CoV-2Interaction studiesReceptor-binding domainBinding domainMembrane biologyBiological processesNatural membranesMembrane model systemsMembrane componentsMembrane bilayerNative membrane componentsProteinExtracellular vesiclesCorrect orientationBiophysical questionsHuman receptor