cDNA cloning and immunolocalization of a Na(+)-H+ exchanger in LLC-PK1 renal epithelial cells
Reilly RF, Hildebrandt F, Biemesderfer D, Sardet C, Pouyssegur J, Aronson PS, Slayman CW, Igarashi P. cDNA cloning and immunolocalization of a Na(+)-H+ exchanger in LLC-PK1 renal epithelial cells. American Journal Of Physiology 1991, 261: f1088-f1094. PMID: 1661081, DOI: 10.1152/ajprenal.1991.261.6.f1088.Peer-Reviewed Original ResearchConceptsLLC-PK1 cellsFusion proteinAmino-terminal hydrophobic regionLLC-PK1 renal epithelial cellsHydrophobic regionAmino acid sequenceConfluent LLC-PK1 cellsRelative molecular massRenal epithelial cellsSignificant homologyHuman proteinsCDNA cloningAcid sequenceNucleotide identityHuman sequenceLibrary screeningCDNAMolecular massPolymerase chain reactionAmino acidsApical membraneProteinConfocal microscopyHydrophilic domainsLLC-PK1Molecular biology of renal Na(+)-H+ exchangers.
Igarashi P, Reilly RF, Hildebrandt F, Biemesderfer D, Rebouças NA, Slayman CW, Aronson PS. Molecular biology of renal Na(+)-H+ exchangers. Kidney International Supplement 1991, 33: s84-9. PMID: 1653876.Commentaries, Editorials and LettersConceptsKb cDNALLC-PK1 cellsSteady-state transcript levelsPlasma membrane proteinsMembrane-associated domainSequence of cDNACDNA library screeningAmino acid sequenceExchanger gene expressionNorthern blot analysisEvolutionary conservationRenal epithelial cellsCytoplasmic domainMembrane proteinsRegulation of intracellularAcid sequenceTranscript levelsUntranslated regionGene expressionSimilar transcriptsCDNALibrary screeningMolecular biologyPolymerase chain reactionBlot analysis