2009
POSH Stimulates the Ubiquitination and the Clathrin-independent Endocytosis of ROMK1 Channels*
Lin DH, Yue P, Pan CY, Sun P, Zhang X, Han Z, Roos M, Caplan M, Giebisch G, Wang WH. POSH Stimulates the Ubiquitination and the Clathrin-independent Endocytosis of ROMK1 Channels*. Journal Of Biological Chemistry 2009, 284: 29614-29624. PMID: 19710010, PMCID: PMC2785594, DOI: 10.1074/jbc.m109.041582.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsBiological TransportCell LineClathrinDynaminsEpithelial Sodium ChannelsGene Expression RegulationHumansKidney Tubules, CollectingOocytesPotassium Channels, Inwardly RectifyingProtein Sorting SignalsProtein Structure, TertiaryRatsRats, Sprague-DawleyUbiquitinationUbiquitin-Protein LigasesXenopus laevisConceptsHEK293T cellsClathrin-independent endocytosisE3 ubiquitin ligaseUbiquitin ligaseGlutathione S-transferase pulldown experimentsROMK1 channelsT cellsTyrosine-based internalization signalPotassium currentROMK channelsDominant-negative dynaminImmunoprecipitation of lysatesInternalization signalInhibitory effectPulldown experimentsScaffold proteinUbiquitination assaysRING domainUbiquitinationN-terminusGamma subunitsAmino acidsENaC-alphaROMK1Tissue lysates
2008
Expression of Tetraspan Protein CD63 Activates Protein-tyrosine Kinase (PTK) and Enhances the PTK-induced Inhibition of ROMK Channels*
Lin D, Kamsteeg EJ, Zhang Y, Jin Y, Sterling H, Yue P, Roos M, Duffield A, Spencer J, Caplan M, Wang WH. Expression of Tetraspan Protein CD63 Activates Protein-tyrosine Kinase (PTK) and Enhances the PTK-induced Inhibition of ROMK Channels*. Journal Of Biological Chemistry 2008, 283: 7674-7681. PMID: 18211905, DOI: 10.1074/jbc.m705574200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDBenzoquinonesCSK Tyrosine-Protein KinaseEnzyme InhibitorsFemaleGene Expression RegulationHumansKidney CortexKidney MedullaLactams, MacrocyclicMaleOocytesOrgan SpecificityPatch-Clamp TechniquesPhosphorylationPlatelet Membrane GlycoproteinsPotassium Channels, Inwardly RectifyingProtein-Tyrosine KinasesProto-Oncogene ProteinsRatsRats, Sprague-DawleyReceptor-Like Protein Tyrosine Phosphatases, Class 4RifabutinSrc-Family KinasesTetraspanin 30TransfectionXenopus laevisConceptsExpression of CD63T cellsOuter medullaRenal cortexROMK channelsProtein tyrosine kinasesC-SrcRole of CD63Potassium restrictionROMK activityPotassium currentTwo-electrode voltage clampRat kidneyDecreased expressionImmunocytochemical stainingROMK1 channelsInhibitory effectMedullaNative rat kidneyCD63Voltage clampCortexRPTPalphaTyrosine phosphorylationHerbimycin A