1999
Insulin-like growth factor I stimulates cardiac myosin heavy chain and actin synthesis in the awake rat
Young L, Renfu Y, Hu X, Chong S, Hasan S, Jacob R, Sherwin R. Insulin-like growth factor I stimulates cardiac myosin heavy chain and actin synthesis in the awake rat. American Journal Of Physiology 1999, 276: e143-e150. PMID: 9886960, DOI: 10.1152/ajpendo.1999.276.1.e143.Peer-Reviewed Original ResearchConceptsInsulin-like growth factor IGrowth factor IAwake ratsMyosin heavy chainFactor IContractile protein synthesisIGF-I infusionLow-dose IGFMixed cardiac proteinsCardiac myosin heavy chainMyosin synthesisBlood pressureOvernight fastIntravenous infusionRight ventricleHeavy chainHeart rateHypoglycemic effectFed ratsIGFRatsDirect actionProtein synthesisSalineCardiac proteins
1996
IGF-I stimulation of muscle protein synthesis in the awake rat: permissive role of insulin and amino acids
Jacob R, Hu X, Niederstock D, Hasan S, McNulty PH, Sherwin RS, Young LH. IGF-I stimulation of muscle protein synthesis in the awake rat: permissive role of insulin and amino acids. American Journal Of Physiology 1996, 270: e60-e66. PMID: 8772474, DOI: 10.1152/ajpendo.1996.270.1.e60.Peer-Reviewed Original ResearchConceptsMuscle protein synthesisInsulin replacementPlasma insulinAwake ratsInsulin-like growth factor ICapacity of IGFIGF-I infusionInfusion of IGFAction of IGFSaline control valuesGrowth factor IIGF-I stimulationProtein synthesisIntravenous infusionPlasma concentrationsInsulin concentrationsAmino acid concentrationsIGFPermissive roleInfusionFactor IControl valuesInsulinRatsAmino acids
1995
Hyperinsulinemia inhibits myocardial protein degradation in patients with cardiovascular disease and insulin resistance.
McNulty P, Louard R, Deckelbaum L, Zaret B, Young L. Hyperinsulinemia inhibits myocardial protein degradation in patients with cardiovascular disease and insulin resistance. Circulation 1995, 92: 2151-6. PMID: 7554195, DOI: 10.1161/01.cir.92.8.2151.Peer-Reviewed Original ResearchConceptsMyocardial protein degradationInsulin infusionCardiovascular diseaseMyocardial protein synthesisInsulin resistancePhenylalanine balanceMyocardial hypertrophyWhole-body glucose metabolismCoronary artery diseaseIschemic heart diseasePlasma insulin concentrationNet phenylalanine balanceWhole-body carbohydrate metabolismEffect of insulinMyocardial protein metabolismArtery diseaseAcute hyperinsulinemiaProtein synthesisHeart diseaseInsulin concentrationsPlasma concentrationsAntiproteolytic actionHyperinsulinemiaGlucose metabolismConstant infusion
1993
Protein synthesis in pulmonary arteries from rats exposed to hyperoxia
Stirewalt WS, Coflesky JT, Young LH, Evans JN. Protein synthesis in pulmonary arteries from rats exposed to hyperoxia. American Journal Of Physiology 1993, 264: l74-l79. PMID: 8430819, DOI: 10.1152/ajplung.1993.264.1.l74.Peer-Reviewed Original Research
1992
Physiological hyperinsulinemia inhibits myocardial protein degradation in vivo in the canine heart.
Young LH, Dahl DM, Rauner D, Barrett EJ. Physiological hyperinsulinemia inhibits myocardial protein degradation in vivo in the canine heart. Circulation Research 1992, 71: 393-400. PMID: 1628395, DOI: 10.1161/01.res.71.2.393.Peer-Reviewed Original ResearchConceptsMyocardial protein degradationProtein degradationProtein synthesisMyocardial protein turnoverAmino acid replacementsAcid replacementsProtein turnoverEssential amino acidsAmino acidsPhenylalanine balanceAmino acid concentrationsNet myocardial releaseSpecific activityPhenylalanineVivoDegradationAnimalsBasal rateIsotopic steady statePhysiological hyperinsulinemiaTurnover
1988
Effect of Chronic Diabetes on Myocardial Fuel Metabolism and Insulin Sensitivity
Barrett E, Schwartz R, Young L, Jacob R, Zaret B. Effect of Chronic Diabetes on Myocardial Fuel Metabolism and Insulin Sensitivity. Diabetes 1988, 37: 943-948. PMID: 3290011, DOI: 10.2337/diab.37.7.943.Peer-Reviewed Original ResearchConceptsArterial free fatty acidsFree fatty acidsMyocardial balanceInsulin clampBranched-chain amino acid concentrationsEuglycemic insulin clamp techniqueGlucose uptakeInsulin-induced hypoaminoacidemiaInsulin clamp techniqueInsulin-deficient diabetesPlasma glucose concentrationFuel substrate metabolismSignificant myocardial uptakeEffect of insulinMyocardial fuel metabolismSignificant glucose uptakePhysiologic hyperinsulinemiaBasal periodChronic diabetesDiabetic animalsInsulin sensitivityAcute effectsAdditional dogsAmino acid concentrationsMyocardial uptake
1987
An isotopic method for measurement of muscle protein synthesis and degradation in vivo
Barrett EJ, Revkin JH, Young LH, Zaret BL, Jacob R, Gelfand RA. An isotopic method for measurement of muscle protein synthesis and degradation in vivo. Biochemical Journal 1987, 245: 223-228. PMID: 3663148, PMCID: PMC1148103, DOI: 10.1042/bj2450223.Peer-Reviewed Original ResearchMeSH KeywordsAmino AcidsAnimalsArteriesDogsFemaleFemoral VeinLeucineMaleMuscle ProteinsPhenylalanineTritiumConceptsNet amino acid releaseFemoral venous plasmaTissue blood flowAmino acid releaseMuscle protein synthesisHindlimb flowTissue protein degradationSpecific radioactivityContinuous infusionUptake of leucineMuscle bedArterial circulationClinical studiesPlasma concentrationsDog hindlimbBlood flowProtein synthesisAcid releaseHindlimbProtein turnoverTissue proteinsPhenylalanine incorporationRemoval of tracerMuscle tissueDogsMyocardial amino acid transport by canine sarcolemma vesicles
Young LH, Zaret BL, Barrett EJ. Myocardial amino acid transport by canine sarcolemma vesicles. American Journal Of Physiology 1987, 252: h1070-h1076. PMID: 3035946, DOI: 10.1152/ajpheart.1987.252.6.h1070.Peer-Reviewed Original ResearchConceptsCanine ventricular myocardiumVentricular myocardiumSarcoplasmic reticulumSarcolemma vesiclesLeucine uptakeAmino acid uptakeL-leucineDependent alanine transport activityAmino acid transportX minStimulatory effectPmol xAcid uptakeMammalian heartSaturable functionMyocardiumAcid transportSarcolemmaLeucine transportAlanine transport activityAlanine fluxMembrane vesiclesDifferent membrane preparations