2024
Plateau depolarizations in spontaneously active neurons detected by calcium or voltage imaging
Milicevic K, Ivanova V, Lovic D, Platisa J, Andjus P, Antic S. Plateau depolarizations in spontaneously active neurons detected by calcium or voltage imaging. Scientific Reports 2024, 14: 22787. PMID: 39367010, PMCID: PMC11452489, DOI: 10.1038/s41598-024-70319-4.Peer-Reviewed Original ResearchConceptsCa2+ transientsAction potentialsSpontaneously active neuronsCalcium imaging studiesCa2+ recordingsNeuronal action potentialsDepolarizing envelopeOptical signalsDendritic Ca2AP firingPlateau depolarizationsPyramidal neuronsMembrane depolarizationPlateau potentialsImaging studiesActive neuronsNeuronal culturesPhoton signaturesImaging modalitiesUP statesIn vivo conditionsNeuronsHalf-widthCa2+Depolarization
2023
High-speed low-light in vivo two-photon voltage imaging of large neuronal populations
Platisa J, Ye X, Ahrens A, Liu C, Chen I, Davison I, Tian L, Pieribone V, Chen J. High-speed low-light in vivo two-photon voltage imaging of large neuronal populations. Nature Methods 2023, 20: 1095-1103. PMID: 36973547, PMCID: PMC10894646, DOI: 10.1038/s41592-023-01820-3.Peer-Reviewed Original ResearchConceptsNeuronal populationsLarge neuronal populationsNeural circuit functionTwo-photon voltage imagingAwake behaving miceCalcium imagingBehaving miceVoltage imagingCircuit functionNeuronsShot noise levelShot-noise limitImagingTwo-photon microscopeKilohertz frame ratesDeep-tissue imagingPopulationVoltage indicatorsField of viewMiceShot noise
2017
Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight
Platisa J, Vasan G, Yang A, Pieribone VA. Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight. ACS Chemical Neuroscience 2017, 8: 513-523. PMID: 28045247, PMCID: PMC5355904, DOI: 10.1021/acschemneuro.6b00234.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedCerebral CortexElectric StimulationEmbryo, MammalianGreen Fluorescent ProteinsHEK293 CellsHumansLuminescent ProteinsMiceModels, MolecularMolecular BiologyMutagenesis, Site-DirectedMutationNeuronsPatch-Clamp TechniquesRecombinant Fusion ProteinsTransfectionVoltage-Sensitive Dye Imaging
2016
A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices
Abdelfattah AS, Farhi SL, Zhao Y, Brinks D, Zou P, Ruangkittisakul A, Platisa J, Pieribone VA, Ballanyi K, Cohen AE, Campbell RE. A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices. Journal Of Neuroscience 2016, 36: 2458-2472. PMID: 26911693, PMCID: PMC4764664, DOI: 10.1523/jneurosci.3484-15.2016.Peer-Reviewed Original ResearchConceptsVoltage indicatorsBlue-shifted channelrhodopsinRed-shifted fluorescent proteinsFluorescent voltage indicatorsWide-field fluorescence microscopyBlue excitationOptical imagingOptical electrophysiologyLow phototoxicityAutofluorescent backgroundLight photoactivationSingle-trial recordingsTemporal resolutionChannelrhodopsinIntrinsic advantagesExcitationVoltage oscillationsFluorescence microscopyOscillationsGreen indicatorsChromophoreMicroscopyResolution
2013
Genetically Targeted Optical Electrophysiology in Intact Neural Circuits
Cao G, Platisa J, Pieribone VA, Raccuglia D, Kunst M, Nitabach MN. Genetically Targeted Optical Electrophysiology in Intact Neural Circuits. Cell 2013, 154: 904-913. PMID: 23932121, PMCID: PMC3874294, DOI: 10.1016/j.cell.2013.07.027.Peer-Reviewed Original ResearchConceptsIntact neural circuitsNeural circuitsIntact brain tissueMembrane potentialNeuronal information processingNervous systemAction potentialsBrain tissueNeuronsStudy of intracellularElectrical activityKey cellular parametersMultiple neuronsElectrical eventsSubthreshold eventsNeurite branchesOptical electrophysiologyReliable recordingCellular parametersVoltage indicatorsFluorescent voltage indicatorsBrain
2012
A Fluorescent, Genetically-Encoded Voltage Probe Capable of Resolving Action Potentials
Barnett L, Platisa J, Popovic M, Pieribone VA, Hughes T. A Fluorescent, Genetically-Encoded Voltage Probe Capable of Resolving Action Potentials. PLOS ONE 2012, 7: e43454. PMID: 22970127, PMCID: PMC3435330, DOI: 10.1371/journal.pone.0043454.Peer-Reviewed Original ResearchSingle Action Potentials and Subthreshold Electrical Events Imaged in Neurons with a Fluorescent Protein Voltage Probe
Jin L, Han Z, Platisa J, Wooltorton JR, Cohen LB, Pieribone VA. Single Action Potentials and Subthreshold Electrical Events Imaged in Neurons with a Fluorescent Protein Voltage Probe. Neuron 2012, 75: 779-785. PMID: 22958819, PMCID: PMC3439164, DOI: 10.1016/j.neuron.2012.06.040.Peer-Reviewed Original Research