2024
Plateau depolarizations in spontaneously active neurons detected by calcium or voltage imaging
Milicevic K, Ivanova V, Lovic D, Platisa J, Andjus P, Antic S. Plateau depolarizations in spontaneously active neurons detected by calcium or voltage imaging. Scientific Reports 2024, 14: 22787. PMID: 39367010, PMCID: PMC11452489, DOI: 10.1038/s41598-024-70319-4.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsCalciumCalcium SignalingCells, CulturedMiceNeuronsPyramidal CellsConceptsCa2+ transientsAction potentialsSpontaneously active neuronsCalcium imaging studiesCa2+ recordingsNeuronal action potentialsDepolarizing envelopeOptical signalsDendritic Ca2AP firingPlateau depolarizationsPyramidal neuronsMembrane depolarizationPlateau potentialsImaging studiesActive neuronsNeuronal culturesPhoton signaturesImaging modalitiesUP statesIn vivo conditionsNeuronsHalf-widthCa2+Depolarization
2017
Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight
Platisa J, Vasan G, Yang A, Pieribone VA. Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight. ACS Chemical Neuroscience 2017, 8: 513-523. PMID: 28045247, PMCID: PMC5355904, DOI: 10.1021/acschemneuro.6b00234.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedCerebral CortexElectric StimulationEmbryo, MammalianGreen Fluorescent ProteinsHEK293 CellsHumansLuminescent ProteinsMiceModels, MolecularMolecular BiologyMutagenesis, Site-DirectedMutationNeuronsPatch-Clamp TechniquesRecombinant Fusion ProteinsTransfectionVoltage-Sensitive Dye Imaging
2016
A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices
Abdelfattah AS, Farhi SL, Zhao Y, Brinks D, Zou P, Ruangkittisakul A, Platisa J, Pieribone VA, Ballanyi K, Cohen AE, Campbell RE. A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices. Journal Of Neuroscience 2016, 36: 2458-2472. PMID: 26911693, PMCID: PMC4764664, DOI: 10.1523/jneurosci.3484-15.2016.Peer-Reviewed Original ResearchConceptsVoltage indicatorsBlue-shifted channelrhodopsinRed-shifted fluorescent proteinsFluorescent voltage indicatorsWide-field fluorescence microscopyBlue excitationOptical imagingOptical electrophysiologyLow phototoxicityAutofluorescent backgroundLight photoactivationSingle-trial recordingsTemporal resolutionChannelrhodopsinIntrinsic advantagesExcitationVoltage oscillationsFluorescence microscopyOscillationsGreen indicatorsChromophoreMicroscopyResolution