2002
Applying Hidden Markov Models to the Analysis of Single Ion Channel Activity
Venkataramanan L, Sigworth F. Applying Hidden Markov Models to the Analysis of Single Ion Channel Activity. Biophysical Journal 2002, 82: 1930-1942. PMID: 11916851, PMCID: PMC1301989, DOI: 10.1016/s0006-3495(02)75542-2.Peer-Reviewed Original ResearchConceptsCorrelated background noiseHidden Markov ModelDigital inverse filterMarkov model parametersDiscrete timeBaum-Welch algorithmMarkov modelComputational intensityDeterministic interferenceModel parametersInverse filterMultiple data setsAlgorithmPrevious resultsPractical applicationsNoise ratioState transitionsSharp frequencyData setsChannel dataSingle ion channel currentsNoiseExtensionRandomnessBackground noise
1998
Activation of Shaker Potassium Channels
Schoppa N, Sigworth F. Activation of Shaker Potassium Channels. The Journal Of General Physiology 1998, 111: 295-311. PMID: 9450945, PMCID: PMC2222768, DOI: 10.1085/jgp.111.2.295.Peer-Reviewed Original ResearchActivation of Shaker Potassium Channels
Schoppa N, Sigworth F. Activation of Shaker Potassium Channels. The Journal Of General Physiology 1998, 111: 271-294. PMID: 9450944, PMCID: PMC2222764, DOI: 10.1085/jgp.111.2.271.Peer-Reviewed Original ResearchActivation of Shaker Potassium Channels
Schoppa N, Sigworth F. Activation of Shaker Potassium Channels. The Journal Of General Physiology 1998, 111: 313-342. PMID: 9450946, PMCID: PMC2222769, DOI: 10.1085/jgp.111.2.313.Peer-Reviewed Original Research
1996
Impaired slow inactivation in mutant sodium channels
Cummins T, Sigworth F. Impaired slow inactivation in mutant sodium channels. Biophysical Journal 1996, 71: 227-236. PMID: 8804606, PMCID: PMC1233474, DOI: 10.1016/s0006-3495(96)79219-6.Peer-Reviewed Original ResearchConceptsRat skeletal muscle channelsSodium currentProlonged depolarizationSlow inactivationPersistent sodium currentMaximal sodium currentSkeletal muscle channelsMuscle paralysisSkeletal muscle fibersMutant sodium channelsSustained currentHyperkalemic periodic paralysisM mutationPeriodic paralysisSodium channelsMuscle channelsMuscle fibersParalysisFast inactivationWild-type channelsMutant channelsDepolarization
1994
Oxygen deprivation activates an ATP-inhibitable K+ channel in substantia nigra neurons
Jiang C, Sigworth F, Haddad G. Oxygen deprivation activates an ATP-inhibitable K+ channel in substantia nigra neurons. Journal Of Neuroscience 1994, 14: 5590-5602. PMID: 8083755, PMCID: PMC6577106, DOI: 10.1523/jneurosci.14-09-05590.1994.Peer-Reviewed Original ResearchConceptsSubstantia nigra neuronsRat substantia nigra neuronsEffects of hypoxiaGlibenclamide binding sitesEffect of ATPCell-attached patchesNeuronal survivalKATP channelsResult of Ca2Different subtypesOutward currentsNeuronal structuresFree Ca2HypoxiaMetabolic stressNeuronsDeleterious changesSingle-channel currentsChannel activityO2 deprivationInternal ATPOxygen deprivationActivationHigh Ca2Membrane potential
1993
Functional consequences of a Na+ channel mutation causing hyperkalemic periodic paralysis
Cummins T, Zhou J, Sigworth F, Ukomadu C, Stephan M, Ptáčk L, Agnew W. Functional consequences of a Na+ channel mutation causing hyperkalemic periodic paralysis. Neuron 1993, 10: 667-678. PMID: 8386527, DOI: 10.1016/0896-6273(93)90168-q.Peer-Reviewed Original ResearchConceptsHyperkalemic periodic paralysisFifth transmembrane segmentHuman embryonic kidney 293 cellsSingle base pair substitutionsEmbryonic kidney 293 cellsKidney 293 cellsBase pair substitutionsTransmembrane segmentsHuman mutationsChannel cDNARat channelHuman skeletal muscleFunctional consequencesPair substitutionsSecond domainCorresponding regionChannel mutationsGenetic defectsMutationsSkeletal musclePeriodic paralysisPatch-clamp recordingsCDNARat musclePedigreeFluctuations in ion channel gating currents. Analysis of nonstationary shot noise
Crouzy S, Sigworth F. Fluctuations in ion channel gating currents. Analysis of nonstationary shot noise. Biophysical Journal 1993, 64: 68-76. PMID: 8381683, PMCID: PMC1262303, DOI: 10.1016/s0006-3495(93)81341-9.Peer-Reviewed Original Research
1992
[52] Analysis of nonstationary single-channel currents
Sigworth F, Zhou J. [52] Analysis of nonstationary single-channel currents. Methods In Enzymology 1992, 207: 746-762. PMID: 1382211, DOI: 10.1016/0076-6879(92)07054-r.Peer-Reviewed Original Research
1991
Open channel noise. VI. Analysis of amplitude histograms to determine rapid kinetic parameters
Heinemann S, Sigworth F. Open channel noise. VI. Analysis of amplitude histograms to determine rapid kinetic parameters. Biophysical Journal 1991, 60: 577-587. PMID: 1718467, PMCID: PMC1260101, DOI: 10.1016/s0006-3495(91)82087-2.Peer-Reviewed Original ResearchChanges in channel properties of acetylcholine receptors during the time course of thiol chemical modifications
Bouzat C, Barrantes F, Sigworth F. Changes in channel properties of acetylcholine receptors during the time course of thiol chemical modifications. Pflügers Archiv - European Journal Of Physiology 1991, 418: 51-61. PMID: 2041725, DOI: 10.1007/bf00370451.Peer-Reviewed Original ResearchConceptsSingle acetylcholine receptor channelsHigh agonist concentrationsPatch-clamp techniqueOpen timeConcentration of NEMSilent periodAcetylcholine receptorsN-ethylmaleimideAcetylcholine receptor channelsAgonist concentrationsChannel open probabilityReceptor channelsAChRMM N-ethylmaleimideTime of exposureRate of occurrenceSingle-channel currentsTime courseNEM treatmentControl receptorsReceptorsSignificant changesTreatmentShort openingsSlight reduction
1990
Yet another approach to the dwell-time omission problem of single-channel analysis
Crouzy S, Sigworth F. Yet another approach to the dwell-time omission problem of single-channel analysis. Biophysical Journal 1990, 58: 731-743. PMID: 1698473, PMCID: PMC1281014, DOI: 10.1016/s0006-3495(90)82416-4.Peer-Reviewed Original Research
1989
Primary structure and functional expression of a mammalian skeletal muscle sodium channel
Trimmer J, Cooperman S, Tomiko S, Zhou J, Crean S, Boyle M, Kalen R, Sheng Z, Barchi R, Sigworth F, Goodman R, Agnew W, Mandel G. Primary structure and functional expression of a mammalian skeletal muscle sodium channel. Neuron 1989, 3: 33-49. PMID: 2559760, DOI: 10.1016/0896-6273(89)90113-x.Peer-Reviewed Original ResearchConceptsAlpha subunitHomologous domains IAmino acid segmentSodium channelsSkeletal muscle sodium channelsNorthern blot analysisSkeletal muscleMuscle sodium channelsVoltage-sensitive sodium channelsPrimary structureFunctional expressionDomain IAcid segmentNative channelsSynthetic RNAXenopus oocytesRat muscle fibersBlot analysisRat skeletal muscleEel electroplaxRat brainSubunitsMuscle fibersMuscleCDNA
1988
Open channel noise. IV. Estimation of rapid kinetics of formamide block in gramicidin A channels
Heinemann S, Sigworth F. Open channel noise. IV. Estimation of rapid kinetics of formamide block in gramicidin A channels. Biophysical Journal 1988, 54: 757-764. PMID: 2465033, PMCID: PMC1330382, DOI: 10.1016/s0006-3495(88)83013-3.Peer-Reviewed Original ResearchConceptsBiological ion channelsGramicidin A channelsTime resolutionCurrent histogramFast time scaleOpen channel noiseCharacteristic timeNoise measurementsSingle-channel experimentsNoise experimentsDirect measurementTime scalesA channelsSpectral densityBandwidth limitationsCurrentTheoretical approachMeasurementsIon channelsBlocking processChannelsSingle-channel recordingsSingle-channel currentsNoiseDependence
1987
Genetic Reconstitution of Functional Acetylcholine Receptor Channels in Mouse Fibroblasts
Claudio T, Green W, Hartman D, Hayden D, Paulson H, Sigworth F, Sine S, Swedlund A. Genetic Reconstitution of Functional Acetylcholine Receptor Channels in Mouse Fibroblasts. Science 1987, 238: 1688-1694. PMID: 3686008, DOI: 10.1126/science.3686008.Peer-Reviewed Original ResearchConceptsExpression systemDNA-mediated gene transfer techniquesMouse fibroblast cellsDNA-mediated gene transferLigand-gated ion channelsCell surface AChRsNew expression systemFibroblast cellsTorpedo AChRClonal cell linesGene transfer techniquesForeign genesGene productsGenetic reconstitutionRecombinant DNACellular componentsTorpedo acetylcholine receptorComplementary DNASurface AChRsAcetylcholine receptor channelsFunctional consequencesGene transferIon channelsHomogenous cellsMouse fibroblasts
1986
Open channel noise. II. A test for coupling between current fluctuations and conformational transitions in the acetylcholine receptor
Sigworth F. Open channel noise. II. A test for coupling between current fluctuations and conformational transitions in the acetylcholine receptor. Biophysical Journal 1986, 49: 1041-1046. PMID: 2423148, PMCID: PMC1329684, DOI: 10.1016/s0006-3495(86)83732-8.Peer-Reviewed Original Research
1980
The variance of sodium current fluctuations at the node of Ranvier
Sigworth F. The variance of sodium current fluctuations at the node of Ranvier. The Journal Of Physiology 1980, 307: 97-129. PMID: 6259340, PMCID: PMC1283036, DOI: 10.1113/jphysiol.1980.sp013426.Peer-Reviewed Original Research