Substitution of a hydrophobic residue alters the conformational stability of Shaker K+ channels during gating and assembly
McCormack K, Lin L, Sigworth F. Substitution of a hydrophobic residue alters the conformational stability of Shaker K+ channels during gating and assembly. Biophysical Journal 1993, 65: 1740-1748. PMID: 8274662, PMCID: PMC1225901, DOI: 10.1016/s0006-3495(93)81202-5.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBiophysical PhenomenaBiophysicsDNA, ComplementaryDrosophilaDrug StabilityFemaleIon Channel GatingMembrane PotentialsMolecular Sequence DataMutagenesis, Site-DirectedOocytesPeptidesPotassium ChannelsProtein ConformationShaker Superfamily of Potassium ChannelsThermodynamicsXenopus laevisConceptsUncharged amino acid residuesLeucine heptad repeatWild-type subunitsPosition 370Large hydrophobic residuesAmino acid residuesSequence motifsConservative substitutionsHeptad repeatHydrophobic residuesVoltage-gated channelsLeucine residuesAcid residuesTertiary structureS4 segmentSpecific hydrophobic interactionsHydrophilic residuesResidue altersChannel subunitsInactivation gatingChannel complexSubunitsConformational stabilityConformational transitionResiduesFunctional expression and purification of a homomeric human alpha 1 glycine receptor in baculovirus-infected insect cells.
Cascio M, Schoppa N, Grodzicki R, Sigworth F, Fox R. Functional expression and purification of a homomeric human alpha 1 glycine receptor in baculovirus-infected insect cells. Journal Of Biological Chemistry 1993, 268: 22135-22142. PMID: 8408073, DOI: 10.1016/s0021-9258(20)80658-9.Peer-Reviewed Original ResearchConceptsInsect cellsGlyR proteinSf9 cellsMembrane protein channelsSf9 insect cellsSodium dodecyl sulfate-polyacrylamide gelsDodecyl sulfate-polyacrylamide gelsSulfate-polyacrylamide gelsWhole cell lysatesHuman kidney cell lineShares sequenceKidney cell lineTransient expressionExpression systemStructural homologyChannel proteinsBiophysical characterizationProtein channelsFunctional channelsFunctional expressionMembrane fractionNative channelsCell surfaceGlycine receptorsChloride channels