1991
Changes in channel properties of acetylcholine receptors during the time course of thiol chemical modifications
Bouzat C, Barrantes F, Sigworth F. Changes in channel properties of acetylcholine receptors during the time course of thiol chemical modifications. Pflügers Archiv - European Journal Of Physiology 1991, 418: 51-61. PMID: 2041725, DOI: 10.1007/bf00370451.Peer-Reviewed Original ResearchConceptsSingle acetylcholine receptor channelsHigh agonist concentrationsPatch-clamp techniqueOpen timeConcentration of NEMSilent periodAcetylcholine receptorsN-ethylmaleimideAcetylcholine receptor channelsAgonist concentrationsChannel open probabilityReceptor channelsAChRMM N-ethylmaleimideTime of exposureRate of occurrenceSingle-channel currentsTime courseNEM treatmentControl receptorsReceptorsSignificant changesTreatmentShort openingsSlight reduction
1988
Chapter 11 Establishing a Stable Expression System for Studies of Acetylcholine Receptors
Claudio T, Paulson H, Hartman D, Sine S, Sigworth F. Chapter 11 Establishing a Stable Expression System for Studies of Acetylcholine Receptors. Current Topics In Membranes 1988, 33: 219-247. DOI: 10.1016/s0070-2161(08)60901-6.ChaptersDNA-mediated gene transferGene transferSelectable marker geneStable expression systemsSame cellsMuscle cell lineEqual copy numberAcetylcholine receptorsDifferent cDNAsMarker genesExpression systemMultiple cDNAsCDNACopy numberCultured cellsCell linesRetroviral infectionCellsGenomeReceptorsGenesCalcium phosphate precipitationAChR.