1993
Functional expression and purification of a homomeric human alpha 1 glycine receptor in baculovirus-infected insect cells.
Cascio M, Schoppa N, Grodzicki R, Sigworth F, Fox R. Functional expression and purification of a homomeric human alpha 1 glycine receptor in baculovirus-infected insect cells. Journal Of Biological Chemistry 1993, 268: 22135-22142. PMID: 8408073, DOI: 10.1016/s0021-9258(20)80658-9.Peer-Reviewed Original ResearchConceptsInsect cellsGlyR proteinSf9 cellsMembrane protein channelsSf9 insect cellsSodium dodecyl sulfate-polyacrylamide gelsDodecyl sulfate-polyacrylamide gelsSulfate-polyacrylamide gelsWhole cell lysatesHuman kidney cell lineShares sequenceKidney cell lineTransient expressionExpression systemStructural homologyChannel proteinsBiophysical characterizationProtein channelsFunctional channelsFunctional expressionMembrane fractionNative channelsCell surfaceGlycine receptorsChloride channels
1989
Primary structure and functional expression of a mammalian skeletal muscle sodium channel
Trimmer J, Cooperman S, Tomiko S, Zhou J, Crean S, Boyle M, Kalen R, Sheng Z, Barchi R, Sigworth F, Goodman R, Agnew W, Mandel G. Primary structure and functional expression of a mammalian skeletal muscle sodium channel. Neuron 1989, 3: 33-49. PMID: 2559760, DOI: 10.1016/0896-6273(89)90113-x.Peer-Reviewed Original ResearchConceptsAlpha subunitHomologous domains IAmino acid segmentSodium channelsSkeletal muscle sodium channelsNorthern blot analysisSkeletal muscleMuscle sodium channelsVoltage-sensitive sodium channelsPrimary structureFunctional expressionDomain IAcid segmentNative channelsSynthetic RNAXenopus oocytesRat muscle fibersBlot analysisRat skeletal muscleEel electroplaxRat brainSubunitsMuscle fibersMuscleCDNA