Featured Publications
Enhanced FIB-SEM systems for large-volume 3D imaging
Xu CS, Hayworth KJ, Lu Z, Grob P, Hassan AM, García-Cerdán JG, Niyogi KK, Nogales E, Weinberg RJ, Hess HF. Enhanced FIB-SEM systems for large-volume 3D imaging. ELife 2017, 6: e25916. PMID: 28500755, PMCID: PMC5476429, DOI: 10.7554/elife.25916.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainChlamydomonas reinhardtiiDrosophilaImaging, Three-DimensionalMice, Inbred C57BLMicroscopy, Electron, ScanningNeuronsConceptsFIB-SEM systemFocused Ion Beam Scanning Electron MicroscopyIon beam scanning electron microscopyBeam scanning electron microscopyLong-term system stabilityScanning electron microscopyNovel high-resolution techniqueSystem stabilityFIB-SEMElectron microscopyHigh resolutionHigh-resolution techniquesSmall volume
2020
Chloroplast Sec14-like 1 (CPSFL1) is essential for normal chloroplast development and affects carotenoid accumulation in Chlamydomonas
García-Cerdán JG, Schmid EM, Takeuchi T, McRae I, McDonald KL, Yordduangjun N, Hassan AM, Grob P, Xu CS, Hess HF, Fletcher DA, Nogales E, Niyogi KK. Chloroplast Sec14-like 1 (CPSFL1) is essential for normal chloroplast development and affects carotenoid accumulation in Chlamydomonas. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 12452-12463. PMID: 32404426, PMCID: PMC7275715, DOI: 10.1073/pnas.1916948117.Peer-Reviewed Original ResearchMeSH KeywordsCarotenoidsChlamydomonas reinhardtiiChloroplastsPhotosynthesisPhylogenyPlant ProteinsProtein DomainsConceptsChloroplast developmentCarotenoid accumulationNormal chloroplast developmentBiosynthesis of carotenoidsEndosymbiotic originInsertional mutantsDomain proteinsIsoprenoid precursorsOxygenic photosynthesisChloroplast structureSuch proteinsAuxiliary proteinsCarotenoid transportPhytoene synthesisLow carotenoid contentMutantsCommitted precursorsProteinChlamydomonasEssential roleImpaired biosynthesisCarotenoid contentPhotosynthesisBiosynthesisIon beam scanning electron microscopy