Correlative 3D superresolution fluorescence and electron microscopy reveal the relationship of mitochondrial nucleoids to membranes
Kopek BG, Shtengel G, Xu CS, Clayton DA, Hess HF. Correlative 3D superresolution fluorescence and electron microscopy reveal the relationship of mitochondrial nucleoids to membranes. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 6136-6141. PMID: 22474357, PMCID: PMC3341004, DOI: 10.1073/pnas.1121558109.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsDNA-Binding ProteinsDNA, MitochondrialFibroblastsHigh Mobility Group ProteinsImaging, Three-DimensionalLuminescent ProteinsMiceMicroscopy, ElectronMicroscopy, FluorescenceMicroscopy, InterferenceMitochondriaMitochondrial MembranesMitochondrial ProteinsReproducibility of ResultsConceptsMitochondrial nucleoidsSuperresolution fluorescenceIon beam ablationOptical microscopy techniquesFluorescent protein labelsCellular contextUltrastructural contextDirect imagingBiological questionsBiological processesSpecific proteinsCellular ultrastructureSuperresolution microscopyProtein labelCellular architectureFluorescence microscopyLocalization microscopyEM imagesNucleoidsMicroscopy techniquesProteinSample preparation protocolElectron microscopyVivo natureMicroscopy