Cloning, embryonic expression, and alternative splicing of a murine kidney-specific Na-K-Cl cotransporter
Igarashi P, Heuvel G, Payne J, Forbush B. Cloning, embryonic expression, and alternative splicing of a murine kidney-specific Na-K-Cl cotransporter. American Journal Of Physiology 1995, 269: f405-f418. PMID: 7573490, DOI: 10.1152/ajprenal.1995.269.3.f405.Peer-Reviewed Original ResearchConceptsNa-K-Cl cotransporterExpression of NKCC2In situ hybridizationCassette exonsRenal Na-K-Cl cotransporterOuter medullaAscending limbAlternative splicingInner stripe of outer medullaPotential sites of phosphorylationAdenosine 3',5'-cyclic monophosphate-dependent protein kinaseDays post coitusFull-length cDNAAnchored polymerase chain reactionSites of phosphorylationConsensus splice donorLoop of HenleS-shaped bodiesProtein kinase CNorthern blot analysisPattern of expressionGenomic clonesNKCC2Outer stripeProtein sequences