2018
Region-specific innate antiviral responses of the human epididymis
Browne JA, Leir SH, Eggener SE, Harris A. Region-specific innate antiviral responses of the human epididymis. Molecular And Cellular Endocrinology 2018, 473: 72-78. PMID: 29339104, PMCID: PMC6045438, DOI: 10.1016/j.mce.2018.01.004.Peer-Reviewed Original ResearchConceptsHEE cellsToll-like receptor 3Innate immune response genesRetinoic acid-inducible gene IAcid-inducible gene IType I interferonIFN-β mRNA expressionInnate antiviral responseImmune response genesIFN-β secretionHuman epididymisAntiviral response pathwaysLike receptorsImpairs fertilityCauda regionsI interferonViral infectionAntiviral responseReceptor 3MRNA expressionEpithelial cellsNuclear translocationGene IEnhanced responseHuman epididymis epithelial cells
2014
Open chromatin mapping identifies transcriptional networks regulating human epididymis epithelial function
Browne JA, Yang R, Song L, Crawford GE, Leir SH, Harris A. Open chromatin mapping identifies transcriptional networks regulating human epididymis epithelial function. Molecular Human Reproduction 2014, 20: 1198-1207. PMID: 25180270, PMCID: PMC4235575, DOI: 10.1093/molehr/gau075.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid MotifsBinding SitesCell LineChromatin Assembly and DisassemblyEpididymisEpithelial CellsGene Expression ProfilingGene Expression RegulationGene Regulatory NetworksGenome-Wide Association StudyHigh-Throughput Nucleotide SequencingHumansMalePAX2 Transcription FactorRNA InterferenceTranscription, GeneticTransfectionConceptsTranscription factor-binding sitesOpen chromatinREP cellsEpididymis epitheliumCoordinated gene expressionGenome-wide mapsSperm cell maturationHigh-throughput sequencingRNA-seq analysisCritical transcriptional regulatorPotential regulatory elementsFactor-binding sitesHEE cellsDNase I digestionRole of PAX2Chromatin mappingUrogenital tract developmentTranscriptional networksTranscriptional regulatorsCoordinated expressionRegulatory elementsMultiple genesGene expressionSilico analysisChromatin