2020
Functional genomics analysis of human colon organoids identifies key transcription factors
Yin S, Ray G, Kerschner JL, Hao S, Perez A, Drumm ML, Browne JA, Leir SH, Longworth M, Harris A. Functional genomics analysis of human colon organoids identifies key transcription factors. Physiological Genomics 2020, 52: 234-244. PMID: 32390556, PMCID: PMC7311676, DOI: 10.1152/physiolgenomics.00113.2019.Peer-Reviewed Original ResearchConceptsHuman intestinal organoidsOpen chromatinTranscription factorsOpen chromatin profilesGenome-wide analysisFunctional genomic analysisCaudal type homeobox 2Open chromatin peaksKey transcription factorIntestinal epitheliumIntestinal epithelial differentiationChromatin profilesMolecular divergenceFunctional genomicsATAC-seqMotif analysisTranscriptome mappingGenomic analysisRNA-seqGenomic signaturesHuman colon organoidsFunctional overlapEpithelial transport processesType homeobox 2Organoid biology
2015
Expression profiles of human epididymis epithelial cells reveal the functional diversity of caput, corpus and cauda regions
Browne JA, Yang R, Leir SH, Eggener SE, Harris A. Expression profiles of human epididymis epithelial cells reveal the functional diversity of caput, corpus and cauda regions. Molecular Human Reproduction 2015, 22: 69-82. PMID: 26612782, PMCID: PMC4733224, DOI: 10.1093/molehr/gav066.Peer-Reviewed Original ResearchConceptsCultured epithelial cellsEpididymis functionExpression profilesHuman epididymis epithelial cellsSperm maturationDefense response processNormal sperm maturationEpithelial cellsProcess enrichment analysisGene expression profilesLuminal environmentUrogenital tract developmentFunctional diversityTranscriptional profilingCauda cellsTranscription factorsBioinformatics toolsBiological replicatesMale genital ductsSimilar transcriptomesMolecular basisEnrichment analysisHormonal signalsSAMPLES/MATERIALSDifferential expression