2021
BACH1, the master regulator of oxidative stress, has a dual effect on CFTR expression
NandyMazumdar M, Paranjapye A, Browne J, Yin S, Leir SH, Harris A. BACH1, the master regulator of oxidative stress, has a dual effect on CFTR expression. Biochemical Journal 2021, 478: 3741-3756. PMID: 34605540, PMCID: PMC8589331, DOI: 10.1042/bcj20210252.Peer-Reviewed Original ResearchMeSH KeywordsBasic-Leucine Zipper Transcription FactorsCell Line, TumorCell ProliferationCystic Fibrosis Transmembrane Conductance RegulatorEpithelial CellsGene Expression ProfilingGene Expression RegulationGlutamate-Cysteine LigaseGlutathioneHeme Oxygenase-1HumansHydrogen PeroxideNF-E2-Related Factor 2Oxidative StressOxygenPromoter Regions, GeneticRNA, Small InterferingSignal TransductionConceptsCis-regulatory elementsTranscription factorsCFTR expressionMaster regulatorHigher-order chromatin structureOrder chromatin structureMultiple cis-regulatory elementsFine-tune expressionSpecific transcription factorsCystic fibrosis transmembrane conductance regulator (CFTR) geneCFTR gene expressionCell-specific expressionTransmembrane conductance regulator geneOxidative stressArchitectural proteinsChromatin structureLocus architectureTune expressionBTB domainCNC homolog 1SiRNA screenRegulator geneGene promoterEnvironmental cuesPhysiological oxygen
2020
Functional genomics analysis of human colon organoids identifies key transcription factors
Yin S, Ray G, Kerschner JL, Hao S, Perez A, Drumm ML, Browne JA, Leir SH, Longworth M, Harris A. Functional genomics analysis of human colon organoids identifies key transcription factors. Physiological Genomics 2020, 52: 234-244. PMID: 32390556, PMCID: PMC7311676, DOI: 10.1152/physiolgenomics.00113.2019.Peer-Reviewed Original ResearchConceptsHuman intestinal organoidsOpen chromatinTranscription factorsOpen chromatin profilesGenome-wide analysisFunctional genomic analysisCaudal type homeobox 2Open chromatin peaksKey transcription factorIntestinal epitheliumIntestinal epithelial differentiationChromatin profilesMolecular divergenceFunctional genomicsATAC-seqMotif analysisTranscriptome mappingGenomic analysisRNA-seqGenomic signaturesHuman colon organoidsFunctional overlapEpithelial transport processesType homeobox 2Organoid biology
2008
TGF-β activates ERK5 in human renal epithelial cells
Browne JA, Pearson AL, Zahr RA, Niculescu-Duvaz I, Baines DL, Dockrell ME. TGF-β activates ERK5 in human renal epithelial cells. Biochemical And Biophysical Research Communications 2008, 373: 440-444. PMID: 18588859, DOI: 10.1016/j.bbrc.2008.06.058.Peer-Reviewed Original ResearchMeSH KeywordsCell Line, TumorEpithelial CellsHumansImidazolesKidneyMADS Domain ProteinsMEF2 Transcription FactorsMitogen-Activated Protein Kinase 7Myogenic Regulatory FactorsP38 Mitogen-Activated Protein KinasesProtein Kinase InhibitorsProtein Serine-Threonine KinasesPyridinesReceptor, Transforming Growth Factor-beta Type IReceptors, Transforming Growth Factor betaTransforming Growth Factor betaConceptsExtracellular signal-regulated kinase 5Epidermal growth factorMAP kinaseERK5 activationMyocyte enhancer factor 2Epithelial cell phenotypeP38 MAP kinase inhibitorRenal epithelial cellsMAP kinase inhibitorHuman renal epithelial cellsEmbryonic lethalityGrowth factorExtracellular signalsSB 202190Cell differentiationKinase 5Human PTECsPhospho-ERK5Cell phenotypeFactor 2KinaseEpithelial cellsKinase inhibitorsReceptor activityActivation