2015
Expression profiles of human epididymis epithelial cells reveal the functional diversity of caput, corpus and cauda regions
Browne JA, Yang R, Leir SH, Eggener SE, Harris A. Expression profiles of human epididymis epithelial cells reveal the functional diversity of caput, corpus and cauda regions. Molecular Human Reproduction 2015, 22: 69-82. PMID: 26612782, PMCID: PMC4733224, DOI: 10.1093/molehr/gav066.Peer-Reviewed Original ResearchConceptsCultured epithelial cellsEpididymis functionExpression profilesHuman epididymis epithelial cellsSperm maturationDefense response processNormal sperm maturationEpithelial cellsProcess enrichment analysisGene expression profilesLuminal environmentUrogenital tract developmentFunctional diversityTranscriptional profilingCauda cellsTranscription factorsBioinformatics toolsBiological replicatesMale genital ductsSimilar transcriptomesMolecular basisEnrichment analysisHormonal signalsSAMPLES/MATERIALSDifferential expression
2014
Open chromatin mapping identifies transcriptional networks regulating human epididymis epithelial function
Browne JA, Yang R, Song L, Crawford GE, Leir SH, Harris A. Open chromatin mapping identifies transcriptional networks regulating human epididymis epithelial function. Molecular Human Reproduction 2014, 20: 1198-1207. PMID: 25180270, PMCID: PMC4235575, DOI: 10.1093/molehr/gau075.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid MotifsBinding SitesCell LineChromatin Assembly and DisassemblyEpididymisEpithelial CellsGene Expression ProfilingGene Expression RegulationGene Regulatory NetworksGenome-Wide Association StudyHigh-Throughput Nucleotide SequencingHumansMalePAX2 Transcription FactorRNA InterferenceTranscription, GeneticTransfectionConceptsTranscription factor-binding sitesOpen chromatinREP cellsEpididymis epitheliumCoordinated gene expressionGenome-wide mapsSperm cell maturationHigh-throughput sequencingRNA-seq analysisCritical transcriptional regulatorPotential regulatory elementsFactor-binding sitesHEE cellsDNase I digestionRole of PAX2Chromatin mappingUrogenital tract developmentTranscriptional networksTranscriptional regulatorsCoordinated expressionRegulatory elementsMultiple genesGene expressionSilico analysisChromatin