2018
A novel transcriptional network for the androgen receptor in human epididymis epithelial cells
Yang R, Browne JA, Eggener SE, Leir SH, Harris A. A novel transcriptional network for the androgen receptor in human epididymis epithelial cells. Molecular Human Reproduction 2018, 24: 433-443. PMID: 30016502, PMCID: PMC6454485, DOI: 10.1093/molehr/gay029.Peer-Reviewed Original ResearchConceptsAndrogen receptorHEE cellsHuman epididymis epithelial cellsEpithelial cellsAR activationTranscriptional networksEunice Kennedy Shriver National InstituteChIP-seqProstate cancer cell linesPARTICIPANTS/MATERIALSNational InstituteSynthetic androgen R1881ROLE OF CHANCEEpididymis epitheliumDistinct androgen receptorsProstate gland epitheliumRNA-seqRunt-related transcription factor 1Single androgen receptorCancer cell linesSilico motif analysisTranscription factor 1Prostate cancerAndrogen R1881Cis-regulatory elementsRegion-specific innate antiviral responses of the human epididymis
Browne JA, Leir SH, Eggener SE, Harris A. Region-specific innate antiviral responses of the human epididymis. Molecular And Cellular Endocrinology 2018, 473: 72-78. PMID: 29339104, PMCID: PMC6045438, DOI: 10.1016/j.mce.2018.01.004.Peer-Reviewed Original ResearchConceptsHEE cellsToll-like receptor 3Innate immune response genesRetinoic acid-inducible gene IAcid-inducible gene IType I interferonIFN-β mRNA expressionInnate antiviral responseImmune response genesIFN-β secretionHuman epididymisAntiviral response pathwaysLike receptorsImpairs fertilityCauda regionsI interferonViral infectionAntiviral responseReceptor 3MRNA expressionEpithelial cellsNuclear translocationGene IEnhanced responseHuman epididymis epithelial cells
2016
HNF1 regulates critical processes in the human epididymis epithelium
Browne JA, Yang R, Eggener SE, Leir SH, Harris A. HNF1 regulates critical processes in the human epididymis epithelium. Molecular And Cellular Endocrinology 2016, 425: 94-102. PMID: 26808453, PMCID: PMC4799753, DOI: 10.1016/j.mce.2016.01.021.Peer-Reviewed Original ResearchConceptsHepatocyte nuclear factor 1Transcription factorsHuman epididymis epithelial cellsCis-regulatory elementsChIP-seq peaksProcess enrichment analysisHNF1β transcription factorHEE cellsNuclear factor 1Luminal environmentChromatin genomeChromatin immunoprecipitationTranscriptional programsCoordinated expressionRNA-seqEpithelial transportRegulatory elementsTarget genesBioinformatics predictionCritical genesEnrichment analysisDeep sequencingEpididymis functionCritical processSperm maturation
2015
Expression profiles of human epididymis epithelial cells reveal the functional diversity of caput, corpus and cauda regions
Browne JA, Yang R, Leir SH, Eggener SE, Harris A. Expression profiles of human epididymis epithelial cells reveal the functional diversity of caput, corpus and cauda regions. Molecular Human Reproduction 2015, 22: 69-82. PMID: 26612782, PMCID: PMC4733224, DOI: 10.1093/molehr/gav066.Peer-Reviewed Original ResearchConceptsCultured epithelial cellsEpididymis functionExpression profilesHuman epididymis epithelial cellsSperm maturationDefense response processNormal sperm maturationEpithelial cellsProcess enrichment analysisGene expression profilesLuminal environmentUrogenital tract developmentFunctional diversityTranscriptional profilingCauda cellsTranscription factorsBioinformatics toolsBiological replicatesMale genital ductsSimilar transcriptomesMolecular basisEnrichment analysisHormonal signalsSAMPLES/MATERIALSDifferential expression
2014
Characterization of primary cultures of adult human epididymis epithelial cells
Leir SH, Browne JA, Eggener SE, Harris A. Characterization of primary cultures of adult human epididymis epithelial cells. Fertility And Sterility 2014, 103: 647-654.e1. PMID: 25542823, PMCID: PMC4346407, DOI: 10.1016/j.fertnstert.2014.11.022.Peer-Reviewed Original ResearchConceptsHuman epididymis epithelial cells