2021
The Bromodomain Containing 8 (BRD8) transcriptional network in human lung epithelial cells
Browne JA, NandyMazumdar M, Paranjapye A, Leir SH, Harris A. The Bromodomain Containing 8 (BRD8) transcriptional network in human lung epithelial cells. Molecular And Cellular Endocrinology 2021, 524: 111169. PMID: 33476703, PMCID: PMC8035426, DOI: 10.1016/j.mce.2021.111169.Peer-Reviewed Original ResearchConceptsCCCTC-Binding FactorTranscription factorsChIP-seq peaksProcess enrichment analysisArchitectural proteinsChromatin modificationsChromatin accessibilityTranscriptional networksProtein functionLung epithelial cell lineRepressive signalsHuman lung epithelial cellsEpithelial cell lineHuman lung epithelial cell lineEnrichment analysisGene expressionLung epithelial cellsCell cycleInnate immune responseLung epithelial functionCell proliferationAntimicrobial peptidesCell linesEpithelial functionEpithelial cells
2018
Region-specific microRNA signatures in the human epididymis
Browne JA, Leir SH, Eggener SE, Harris A. Region-specific microRNA signatures in the human epididymis. Asian Journal Of Andrology 2018, 20: 539-544. PMID: 30058558, PMCID: PMC6219309, DOI: 10.4103/aja.aja_40_18.Peer-Reviewed Original ResearchConceptsGene expressionSegment-specific gene expressionImportant gene networksRegionalized expression patternsRegionalized gene expressionGene ontology pathwaysSmall noncoding RNAsHuman epididymisEpithelial cellsOntology pathwaysGene networksNoncoding RNAsTarget genesHuman epididymal tissueExpression patternsSperm maturationMiR-573MiRNA expressionMiRNAsDifferentiated functionsMicroRNAsMicroRNA profilesMale fertilityLuminal environmentMiRNA signatureA novel transcriptional network for the androgen receptor in human epididymis epithelial cells
Yang R, Browne JA, Eggener SE, Leir SH, Harris A. A novel transcriptional network for the androgen receptor in human epididymis epithelial cells. Molecular Human Reproduction 2018, 24: 433-443. PMID: 30016502, PMCID: PMC6454485, DOI: 10.1093/molehr/gay029.Peer-Reviewed Original ResearchConceptsAndrogen receptorHEE cellsHuman epididymis epithelial cellsEpithelial cellsAR activationTranscriptional networksEunice Kennedy Shriver National InstituteChIP-seqProstate cancer cell linesPARTICIPANTS/MATERIALSNational InstituteSynthetic androgen R1881ROLE OF CHANCEEpididymis epitheliumDistinct androgen receptorsProstate gland epitheliumRNA-seqRunt-related transcription factor 1Single androgen receptorCancer cell linesSilico motif analysisTranscription factor 1Prostate cancerAndrogen R1881Cis-regulatory elementsA transcription factor network represses CFTR gene expression in airway epithelial cells.
Mutolo MJ, Leir SH, Fossum SL, Browne JA, Harris A. A transcription factor network represses CFTR gene expression in airway epithelial cells. Biochemical Journal 2018, 475: 1323-1334. PMID: 29572268, PMCID: PMC6380350, DOI: 10.1042/bcj20180044.Peer-Reviewed Original ResearchConceptsCystic fibrosisTranscription factorsAirway epitheliumEpithelial cellsCalu-3 lung epithelial cellsPrimary human bronchial epithelial cellsAirway epithelium resultsKrüppel-like factor 5Novel therapeutic targetAirway epithelial cellsEts homologous factorHuman bronchial epithelial cellsTranscription factor networkBronchial epithelial cellsLung epithelial cellsTissue-specific enhancersCystic fibrosis transmembrane conductance regulator (CFTR) geneCFTR gene expressionAirway expressionTransmembrane conductance regulator geneLung diseaseCFTR mRNA levelsPancreatic ductTherapeutic targetCF morbidityRegion-specific innate antiviral responses of the human epididymis
Browne JA, Leir SH, Eggener SE, Harris A. Region-specific innate antiviral responses of the human epididymis. Molecular And Cellular Endocrinology 2018, 473: 72-78. PMID: 29339104, PMCID: PMC6045438, DOI: 10.1016/j.mce.2018.01.004.Peer-Reviewed Original ResearchConceptsHEE cellsToll-like receptor 3Innate immune response genesRetinoic acid-inducible gene IAcid-inducible gene IType I interferonIFN-β mRNA expressionInnate antiviral responseImmune response genesIFN-β secretionHuman epididymisAntiviral response pathwaysLike receptorsImpairs fertilityCauda regionsI interferonViral infectionAntiviral responseReceptor 3MRNA expressionEpithelial cellsNuclear translocationGene IEnhanced responseHuman epididymis epithelial cells
2015
Expression profiles of human epididymis epithelial cells reveal the functional diversity of caput, corpus and cauda regions
Browne JA, Yang R, Leir SH, Eggener SE, Harris A. Expression profiles of human epididymis epithelial cells reveal the functional diversity of caput, corpus and cauda regions. Molecular Human Reproduction 2015, 22: 69-82. PMID: 26612782, PMCID: PMC4733224, DOI: 10.1093/molehr/gav066.Peer-Reviewed Original ResearchConceptsCultured epithelial cellsEpididymis functionExpression profilesHuman epididymis epithelial cellsSperm maturationDefense response processNormal sperm maturationEpithelial cellsProcess enrichment analysisGene expression profilesLuminal environmentUrogenital tract developmentFunctional diversityTranscriptional profilingCauda cellsTranscription factorsBioinformatics toolsBiological replicatesMale genital ductsSimilar transcriptomesMolecular basisEnrichment analysisHormonal signalsSAMPLES/MATERIALSDifferential expression
2014
An Optimized Protocol for Isolating Primary Epithelial Cell Chromatin for ChIP
Browne JA, Harris A, Leir SH. An Optimized Protocol for Isolating Primary Epithelial Cell Chromatin for ChIP. PLOS ONE 2014, 9: e100099. PMID: 24971909, PMCID: PMC4074041, DOI: 10.1371/journal.pone.0100099.Peer-Reviewed Original ResearchConceptsCell typesChromatin immunoprecipitation dataDNA-binding proteinsLysis bufferPrimary human epithelial cellsEpithelial cell typesEpithelial cellsChromatin purificationHuman bronchial epithelial cellsENCODE consortiumHuman epithelial cellsCell chromatinNext-generation sequencingImmunoprecipitation dataCell lysis procedurePrimary human bronchial epithelial cellsChromatinFormaldehyde-fixed cellsBronchial epithelial cellsMembrane lysisSize selectionLysis procedureAdherent cellsCellsLysis step
2008
TGF-β activates ERK5 in human renal epithelial cells
Browne JA, Pearson AL, Zahr RA, Niculescu-Duvaz I, Baines DL, Dockrell ME. TGF-β activates ERK5 in human renal epithelial cells. Biochemical And Biophysical Research Communications 2008, 373: 440-444. PMID: 18588859, DOI: 10.1016/j.bbrc.2008.06.058.Peer-Reviewed Original ResearchMeSH KeywordsCell Line, TumorEpithelial CellsHumansImidazolesKidneyMADS Domain ProteinsMEF2 Transcription FactorsMitogen-Activated Protein Kinase 7Myogenic Regulatory FactorsP38 Mitogen-Activated Protein KinasesProtein Kinase InhibitorsProtein Serine-Threonine KinasesPyridinesReceptor, Transforming Growth Factor-beta Type IReceptors, Transforming Growth Factor betaTransforming Growth Factor betaConceptsExtracellular signal-regulated kinase 5Epidermal growth factorMAP kinaseERK5 activationMyocyte enhancer factor 2Epithelial cell phenotypeP38 MAP kinase inhibitorRenal epithelial cellsMAP kinase inhibitorHuman renal epithelial cellsEmbryonic lethalityGrowth factorExtracellular signalsSB 202190Cell differentiationKinase 5Human PTECsPhospho-ERK5Cell phenotypeFactor 2KinaseEpithelial cellsKinase inhibitorsReceptor activityActivation