An Optimized Protocol for Isolating Primary Epithelial Cell Chromatin for ChIP
Browne JA, Harris A, Leir SH. An Optimized Protocol for Isolating Primary Epithelial Cell Chromatin for ChIP. PLOS ONE 2014, 9: e100099. PMID: 24971909, PMCID: PMC4074041, DOI: 10.1371/journal.pone.0100099.Peer-Reviewed Original ResearchConceptsCell typesChromatin immunoprecipitation dataDNA-binding proteinsLysis bufferPrimary human epithelial cellsEpithelial cell typesEpithelial cellsChromatin purificationHuman bronchial epithelial cellsENCODE consortiumHuman epithelial cellsCell chromatinNext-generation sequencingImmunoprecipitation dataCell lysis procedurePrimary human bronchial epithelial cellsChromatinFormaldehyde-fixed cellsBronchial epithelial cellsMembrane lysisSize selectionLysis procedureAdherent cellsCellsLysis step