2015
Disruption of the mevalonate pathway induces dNTP depletion and DNA damage
Sánchez C, Martín J, Jin JS, Dávalos A, Zhang W, de la Peña G, Martínez-Botas J, Rodríguez-Acebes S, Suárez Y, Hazen MJ, Gómez-Coronado D, Busto R, Cheng YC, Lasunción MA. Disruption of the mevalonate pathway induces dNTP depletion and DNA damage. Biochimica Et Biophysica Acta 2015, 1851: 1240-1253. PMID: 26055626, DOI: 10.1016/j.bbalip.2015.06.001.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateCarboxy-LyasesCell Cycle CheckpointsCell Line, TumorCell ProliferationCheckpoint Kinase 1DeoxyribonucleosidesDNA DamageDNA ReplicationGene Expression RegulationHalogenationHemiterpenesHistonesHL-60 CellsHumansLymphocytesMevalonic AcidOrganophosphorus CompoundsProtein KinasesRNA, Small InterferingSignal TransductionConceptsMevalonate diphosphate decarboxylaseDiphosphate decarboxylaseCell cycle progressionDNA replicationCycle progressionMevalonate pathwayDNA damageDNA damage responseNon-sterol isoprenoidsCell proliferationInhibition of Chk1Cholesterol biosynthesis pathwayMassive cell deathSubsequent DNA damageΓ-H2AX formationCell cycle arrestReplication stressBiosynthesis pathwayΓ-H2AX fociChk1 activationDamage responseIsopentenyl diphosphateMitosis completionCell divisionDNTP depletion
2002
Inhibition of cholesterol biosynthesis by Δ22-unsaturated phytosterols via competitive inhibition of sterol Δ24-reductase in mammalian cells
FERNÁNDEZ C, SUÁREZ Y, FERRUELO AJ, GÓMEZ-CORONADO D, LASUNCIÓN MA. Inhibition of cholesterol biosynthesis by Δ22-unsaturated phytosterols via competitive inhibition of sterol Δ24-reductase in mammalian cells. Biochemical Journal 2002, 366: 109-119. PMID: 12162789, PMCID: PMC1222779, DOI: 10.1042/bj20011777.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding, CompetitiveCaco-2 CellsCholesterolChromatography, High Pressure LiquidDose-Response Relationship, DrugHL-60 CellsHumansHypolipidemic AgentsKineticsMacrophagesMaleMiceMicrosomes, LiverModels, ChemicalOxidoreductasesPhytosterolsRatsRats, Sprague-DawleyStigmasterolTime FactorsTumor Cells, CulturedConceptsCholesterol biosynthesisCholesterol-lowering actionCholesterol-lowering agentsEffective hypocholesterolaemic agentHL-60 human cell linesRat liver microsomesIntestinal absorptionDietary phytosterolsLiver microsomesHypocholesterolaemic agentsLow intracellular concentrationsCaco-2Inhibition of sterolIncorporation of radioactivityHuman cell linesCell linesInhibitionSaturated side chainIntracellular concentrationCholesterolRelevant concentrationsRadioactivity incorporationPresent studyStrong inhibitionCompetitive inhibition
1999
Cholesterol starvation decreases P34cdc2 kinase activity and arrests the cell cycle at G2
Martínez‐Botas J, Suárez Y, Ferruelo A, Gómez‐Coronado D, Lasunció M. Cholesterol starvation decreases P34cdc2 kinase activity and arrests the cell cycle at G2. The FASEB Journal 1999, 13: 1359-1370. PMID: 10428760, DOI: 10.1096/fasebj.13.11.1359.Peer-Reviewed Original Research
1998
Induction of apoptosis in p53-null HL-60 cells by inhibition of lanosterol 14-α demethylase
Martínez-Botas J, Ferruelo A, Suárez Y, Gómez-Coronado D, Lasunción M. Induction of apoptosis in p53-null HL-60 cells by inhibition of lanosterol 14-α demethylase. Biochimie 1998, 80: 887-894. PMID: 9893947, DOI: 10.1016/s0300-9084(00)88884-7.Peer-Reviewed Original Research