2014
Unanchored K48-Linked Polyubiquitin Synthesized by the E3-Ubiquitin Ligase TRIM6 Stimulates the Interferon-IKKε Kinase-Mediated Antiviral Response
Rajsbaum R, Versteeg GA, Schmid S, Maestre AM, Belicha-Villanueva A, Martínez-Romero C, Patel JR, Morrison J, Pisanelli G, Miorin L, Laurent-Rolle M, Moulton HM, Stein DA, Fernandez-Sesma A, tenOever BR, García-Sastre A. Unanchored K48-Linked Polyubiquitin Synthesized by the E3-Ubiquitin Ligase TRIM6 Stimulates the Interferon-IKKε Kinase-Mediated Antiviral Response. Immunity 2014, 40: 880-895. PMID: 24882218, PMCID: PMC4114019, DOI: 10.1016/j.immuni.2014.04.018.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntiviral AgentsCells, CulturedEnzyme ActivationHumansI-kappa B KinaseInterferon Type IJanus Kinase 1MicePhosphorylationPolyubiquitinRNA InterferenceRNA, Small InterferingSignal TransductionSTAT1 Transcription FactorTripartite Motif ProteinsUbiquitin-Conjugating EnzymesUbiquitin-Protein LigasesConceptsUnanchored K48Polyubiquitin chainsUnanchored polyubiquitin chainsUpregulation of hundredsAntiviral responseIκB kinase epsilonTripartite motif (TRIM) familyIKKε activationMotif familyKinase activationCellular factorsUBE2KReceptor signalingSTAT1 phosphorylationK48Kinase epsilonType I interferonIFN signalingIFN receptor signalingTRIM6Microbial infectionsISG inductionI interferonSignalingIKKε
2010
The NS5 Protein of the Virulent West Nile Virus NY99 Strain Is a Potent Antagonist of Type I Interferon-Mediated JAK-STAT Signaling
Laurent-Rolle M, Boer EF, Lubick KJ, Wolfinbarger JB, Carmody AB, Rockx B, Liu W, Ashour J, Shupert WL, Holbrook MR, Barrett AD, Mason PW, Bloom ME, García-Sastre A, Khromykh AA, Best SM. The NS5 Protein of the Virulent West Nile Virus NY99 Strain Is a Potent Antagonist of Type I Interferon-Mediated JAK-STAT Signaling. Journal Of Virology 2010, 84: 3503-3515. PMID: 20106931, PMCID: PMC2838099, DOI: 10.1128/jvi.01161-09.Peer-Reviewed Original ResearchConceptsPY-STAT1IFN antagonismJAK-STAT signal transductionIFN antagonistAlpha/beta interferonSignal transductionWild-type virusAnalogous residuesIFN-dependent gene expressionGene expressionJAK-STATKunjin virusSingle residueHost innate responseNonstructural protein NS5NS5 proteinSTAT1 phosphorylationSTAT2 degradationPoor suppressorNY99 strainPresence of IFNMutationsIFN-alpha/NS5Beta interferon