2016
P148 Uncovering antibodies to cryptic epitopes: Use of flow cytometry crossmatch to differentiate between functional and denatured epitopes
Mangiola M, Lomago J, Marilyn M, Nichol L, Zern D, McGowan K, Sese D, Bow L, Zeevi A. P148 Uncovering antibodies to cryptic epitopes: Use of flow cytometry crossmatch to differentiate between functional and denatured epitopes. Human Immunology 2016, 77: 145. DOI: 10.1016/j.humimm.2016.07.213.Peer-Reviewed Original ResearchAntigen beadsCryptic epitopesFlow cytometry crossmatchSingle antigen beadsSmall bowel transplantHLA antibody detectionClass II profileBowel transplantCytometry crossmatchDR antigensHLA AbsDR7 allelesPatient beingCrossmatch requestsAntibody detectionSurrogate donorsMolecular typingFlow cytometryExon 2Ab profilesPotential targetPatient cellsEpitopesPatientsPremature stop codon
2012
39-P INCREASING MFI CUTOFFS FOR UNACCEPTABLE ANTIGENS INCREASES ACCESS TO ACTUAL DECEASED DONOR CROSSMATCHES FOR THE SENSITIZED RECIPIENT
Miller M, Alberghini T, Rewinski M, Bow L. 39-P INCREASING MFI CUTOFFS FOR UNACCEPTABLE ANTIGENS INCREASES ACCESS TO ACTUAL DECEASED DONOR CROSSMATCHES FOR THE SENSITIZED RECIPIENT. Human Immunology 2012, 73: 74. DOI: 10.1016/j.humimm.2012.07.165.Peer-Reviewed Original ResearchUnacceptable antigensB cellsB-cell crossmatchPositive flow crossmatchLow-level antibodyHLA class IResultsFifty-sixSensitized recipientsKidney transplantationPositive crossmatchFlow crossmatchVirtual crossmatchDonor workupDP antibodiesNegative control seraLuminex assaysCrossmatchHigh MFI valuesControl seraFlow cytometryMultiple antibodiesClass IRecipientsAntibodiesMatch runs