2016
P054 EDTA treatment of serum eliminates interference in flow cytometric crossmatches
Rewinski M, Ortiz J, Alberghini T, Bow L, Devivo J, Doyle P, Michalski T. P054 EDTA treatment of serum eliminates interference in flow cytometric crossmatches. Human Immunology 2016, 77: 78. DOI: 10.1016/j.humimm.2016.07.119.Peer-Reviewed Original ResearchT cell populationsPatient seraMedian fluorescence intensityT cellsComplement proteinsControl seraUntreated patients' seraFlow cytometric crossmatchDonor cellsSignificant T-cellCell populationsOccasional false positive resultsHigh-titer antibodiesPatient serum samplesCytometric crossmatchFlow crossmatchAntibody testingFalse-positive resultsIgM antibodiesPositive control seraMFI levelsNegative control seraTiter antibodiesSerum samplesSerum
2012
45-P WHEN A DSA IS NOT A DSA: POSITIVE VIRTUAL CROSSMATCHES RESULTING IN ACTUAL NEGATIVE CROSSMATCHES
Rewinski M, Ortiz J, Bared K, Alberghini T, Bow L. 45-P WHEN A DSA IS NOT A DSA: POSITIVE VIRTUAL CROSSMATCHES RESULTING IN ACTUAL NEGATIVE CROSSMATCHES. Human Immunology 2012, 73: 78. DOI: 10.1016/j.humimm.2012.07.171.Peer-Reviewed Original ResearchDonor-specific antibodiesPositive virtual crossmatchVirtual crossmatchMFI levelsUnacceptable antigensClass ILuminex single antigenNegative flow crossmatchKidney transplant recipientsSuccessful kidney transplantationDonor cellsKidney waiting listPotential kidney recipientsHLA class IKidney recipientsTransplant recipientsKidney transplantationFlow crossmatchAntibody testingNegative crossmatchPatient seraAntibody assaysLuminex assaysWaiting listCrossmatch