2007
Phosphorylation at Ser244 by CK1 determines nuclear localization and substrate targeting of PKD2
von Blume J, Knippschild U, Dequiedt F, Giamas G, Beck A, Auer A, Van Lint J, Adler G, Seufferlein T. Phosphorylation at Ser244 by CK1 determines nuclear localization and substrate targeting of PKD2. The EMBO Journal 2007, 26: 4619-4633. PMID: 17962809, PMCID: PMC2080801, DOI: 10.1038/sj.emboj.7601891.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCasein Kinase 1 epsilonCasein Kinase IdeltaCell LineCell Line, TumorCell NucleusChlorocebus aethiopsCOS CellsDNA-Binding ProteinsGastrinsHeLa CellsHistone DeacetylasesHumansNuclear Receptor Subfamily 4, Group A, Member 1PhosphorylationProtein Kinase CProtein Kinase D2Protein KinasesReceptor, Cholecystokinin BReceptors, Cytoplasmic and NuclearReceptors, SteroidSerineTranscription FactorsConceptsProtein kinase D2Nuclear accumulationEfficient phosphorylationCritical post-translational modificationSerine/threonine kinaseZinc finger domainPost-translational modificationsSignal transduction pathwaysG protein-coupled receptorsHuman gastric cancer cellsExport machinerySubstrate targetingHDAC7 phosphorylationActivation loopNuclear substratesThreonine kinaseActive kinaseNuclear exportGastric cancer cellsSubcellular compartmentsTransduction pathwaysNuclear localizationPKD familyPKC etaPhosphorylation
2006
New Role for hPar-1 Kinases EMK and C-TAK1 in Regulating Localization and Activity of Class IIa Histone Deacetylases
Dequiedt F, Martin M, Von Blume J, Vertommen D, Lecomte E, Mari N, Heinen MF, Bachmann M, Twizere JC, Huang MC, Rider MH, Piwnica-Worms H, Seufferlein T, Kettmann R. New Role for hPar-1 Kinases EMK and C-TAK1 in Regulating Localization and Activity of Class IIa Histone Deacetylases. Molecular And Cellular Biology 2006, 26: 7086-7102. PMID: 16980613, PMCID: PMC1592903, DOI: 10.1128/mcb.00231-06.Peer-Reviewed Original ResearchMeSH Keywords14-3-3 ProteinsActive Transport, Cell NucleusAmino Acid SequenceAnimalsBinding SitesCell NucleusCells, CulturedChlorocebus aethiopsCOS CellsCytoplasmHeLa CellsHistone DeacetylasesHumansMolecular Sequence DataPhosphorylationPhosphoserineProtein Serine-Threonine KinasesProtein TransportSubstrate SpecificityConceptsMicrotubule affinity-regulating kinaseClass IIa HDACsN-terminal serine residueClass IIa histoneIIa HDACsC-TAK1IIa histoneSerine residuesMajor developmental programsMARK/ParVariety of kinasesHierarchical phosphorylationRepressive activityNuclear exportRepressive functionDevelopmental programSubcellular localizationNuclear exclusionConstitutive phosphorylationSophisticated regulationPhosphorylationKinaseSpecific signalsHDACsHistones