Transcriptional Activation of the Cyclooxygenase-2 Gene in Endotoxin-treated RAW 264.7 Macrophages*
Wadleigh D, Reddy S, Kopp E, Ghosh S, Herschman H. Transcriptional Activation of the Cyclooxygenase-2 Gene in Endotoxin-treated RAW 264.7 Macrophages*. Journal Of Biological Chemistry 2000, 275: 6259-6266. PMID: 10692422, DOI: 10.1074/jbc.275.9.6259.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsCCAAT-Enhancer-Binding ProteinsCyclic AMPCyclooxygenase 2DNA-Binding ProteinsDrosophila ProteinsEndotoxinsGenes, ReporterI-kappa B ProteinsIsoenzymesJNK Mitogen-Activated Protein KinasesLipopolysaccharidesMacrophagesMAP Kinase Kinase Kinase 1MiceMitogen-Activated Protein KinasesMutationNuclear ProteinsPhosphorylationPromoter Regions, GeneticProstaglandin-Endoperoxide SynthasesProtein Serine-Threonine KinasesProteinsProto-Oncogene Proteins c-junSignal TransductionTranscriptional ActivationConceptsTranscriptional activationCis-acting transcriptional regulatory elementsReporter activityRas-independent pathwayTranscriptional regulatory elementsCis-acting elementsDominant-negative formDominant-negative expression vectorCyclic AMP response elementC-Jun phosphorylationElement-binding proteinAMP response elementImmediate early genesRAW 264.7 macrophagesTranscription factorsRaf-1E-boxRegulatory elementsAlpha mutantsCOX-2 promoterGene expressionCOX-2 reporterExpression vectorCyclic AMP response element-binding proteinC-Jun