2017
Vancomycin Reduces Cell Wall Stiffness and Slows Swim Speed of the Lyme Disease Bacterium
Harman MW, Hamby AE, Boltyanskiy R, Belperron AA, Bockenstedt LK, Kress H, Dufresne ER, Wolgemuth CW. Vancomycin Reduces Cell Wall Stiffness and Slows Swim Speed of the Lyme Disease Bacterium. Biophysical Journal 2017, 112: 746-754. PMID: 28256234, PMCID: PMC5340155, DOI: 10.1016/j.bpj.2016.12.039.Peer-Reviewed Original ResearchMeSH KeywordsAnti-Bacterial AgentsBiomechanical PhenomenaBorrelia burgdorferiCell WallLyme DiseaseMechanical PhenomenaMovementVancomycinConceptsCell wall stiffnessCell wallCell stiffnessCell speedLyme disease bacteriumSpirochete survivalPeriplasmic volumeCell shapeFlat wave shapeSwim speedTick hostsCell growthCell morphologyDisease bacteriumVolume regulationHost tissuesTestable hypothesesPrevious mathematical modelingBleb formationSublethal dosesMotilityBiophysical modelSwimming speedBorrelia burgdorferiSlower swim speed
2012
Spirochete antigens persist near cartilage after murine Lyme borreliosis therapy
Bockenstedt LK, Gonzalez DG, Haberman AM, Belperron AA. Spirochete antigens persist near cartilage after murine Lyme borreliosis therapy. Journal Of Clinical Investigation 2012, 122: 2652-2660. PMID: 22728937, PMCID: PMC3386809, DOI: 10.1172/jci58813.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnti-Bacterial AgentsAntigens, BacterialArthritis, InfectiousBacterial LoadBorrelia burgdorferiCartilageCeftriaxoneDoxycyclineEar, ExternalFemaleFluorescence Recovery After PhotobleachingGreen Fluorescent ProteinsJoint CapsuleLyme DiseaseMiceMice, Inbred C3HMice, Inbred C57BLMice, KnockoutMicroscopy, Fluorescence, MultiphotonMyeloid Differentiation Factor 88PatellaRecombinant ProteinsConceptsNaive miceAntibiotic treatmentIntravital microscopyAntibiotic-refractory Lyme arthritisLyme diseaseTNF-α productionBorrelia burgdorferi antigensB. burgdorferi antigensSpirochete antigenTLR responsivenessInflammatory arthritisAntibiotic therapyLyme arthritisWT miceMusculoskeletal symptomsAntigens persistSlow resolutionImmunodeficient miceMouse modelTissue transplantsPathogen burdenSpirochete DNAInfectious spirochetesLyme borreliosisMice