2001
Alteration in expression of the rat mitochondrial ATPase 6 gene during Pneumocystis carinii infection
Asnicar M, Henegariu O, Shaw M, Goheen M, Bartlett M, Smith J, Lee C. Alteration in expression of the rat mitochondrial ATPase 6 gene during Pneumocystis carinii infection. BMC Microbiology 2001, 1: 8. PMID: 11446902, PMCID: PMC34520, DOI: 10.1186/1471-2180-1-8.Peer-Reviewed Original ResearchConceptsATPase 6 geneMitochondrial ATPase 6 geneHost cellsF0F1-ATP synthase complexTwo-color fluorescentMRNA differential displaySitu hybridizationATPase 6 mRNANorthern blot analysisATPase 6Synthase complexSurfactant protein BNucleotide sequenceDifferential displayDNA fragmentsGenesCell typesClara cellsMolecular levelProtein BBlot analysisType II pneumocytesTotal RNASame cellsRespiratory treeCryptic Translocation Identification in Human and Mouse Using Several Telomeric Multiplex FISH (TM-FISH) Strategies
Henegariu O, Artan S, Greally J, Chen X, Korenberg J, Vance G, Stubbs L, Bray-Ward P, Ward D. Cryptic Translocation Identification in Human and Mouse Using Several Telomeric Multiplex FISH (TM-FISH) Strategies. Laboratory Investigation 2001, 81: 483-491. PMID: 11304567, DOI: 10.1038/labinvest.3780256.Peer-Reviewed Original ResearchConceptsSubtelomeric regionsHuman subtelomeric regionsMouse chromosomesHuman chromosomesP1 clonesMouse probeMultiplex fluorescenceM-FISHChromosomesFISH strategySet of probesStandard fluorescence microscopeSmall rearrangementsSitu hybridizationLabeling strategyCryptic translocationSubtelomeric probesIdiopathic mental retardationFluorescence microscopeKnown markerCentromeresKilobasesImprovements in cytogenetic slide preparation: Controlled chromosome spreading, chemical aging and gradual denaturing
Henegariu O, Heerema N, Wright L, Bray‐Ward P, Ward D, Vance G. Improvements in cytogenetic slide preparation: Controlled chromosome spreading, chemical aging and gradual denaturing. Cytometry 2001, 43: 101-109. PMID: 11169574, DOI: 10.1002/1097-0320(20010201)43:2<101::aid-cyto1024>3.0.co;2-8.Peer-Reviewed Original ResearchConceptsComparative genomic hybridizationClassical banding techniquesSitu hybridization applicationsChromosomal integrityChromosome spreadsMultiplex FISHMolecular cytogeneticsFISH applicationsChromosome spreadingFISH techniqueGenomic hybridizationEnvironmental conditionsSitu hybridizationBanding techniquesChromosomesHybridizationReproducible protocolFISH resultsCytogenetic slidesCell suspensionsSlide preparationFishMetaphase
1998
Characterization of Multiple 12p Rearrangements in Testicular Germ Cell Tumor Cell Line 833K and Its Subclone 64CP by Chromosome Microdissection
Blough R, Vance G, Henegariu O, Smolarek T, Sledge G, Heerema N. Characterization of Multiple 12p Rearrangements in Testicular Germ Cell Tumor Cell Line 833K and Its Subclone 64CP by Chromosome Microdissection. Cancer Genetics 1998, 106: 24-29. PMID: 9772905, DOI: 10.1016/s0165-4608(98)00042-9.Peer-Reviewed Original ResearchConceptsChromosome 12 regionDistal 12pNormal human chromosomesBeta-satellite DNABand-specific probesFISH experimentsChromosome arm 12pChromosome microdissectionChromosomal microdissectionHuman chromosomesPainting probesChromosome 12Stalk regionCisplatin-resistant subclonesSitu hybridizationRDNACommon breakpointFishMicrodissectionSpecific originChromatinRearrangementChromosomesUnusual distributionBreakpointsMolecular cytogenetic analysis of patients with holoprosencephaly and structural rearrangements of 7q
Vance G, Nickerson C, Sarnat L, Zhang A, Henegariu O, Morichon‐Delvallez N, Butler M, Palmer C. Molecular cytogenetic analysis of patients with holoprosencephaly and structural rearrangements of 7q. American Journal Of Medical Genetics 1998, 76: 51-57. PMID: 9508065, PMCID: PMC6775641, DOI: 10.1002/(sici)1096-8628(19980226)76:1<51::aid-ajmg9>3.0.co;2-s.Peer-Reviewed Original ResearchMeSH KeywordsCell LineChromosome AberrationsChromosome DeletionChromosomes, Human, Pair 13Chromosomes, Human, Pair 22Chromosomes, Human, Pair 7CytogeneticsFemaleGenetic MarkersHedgehog ProteinsHoloprosencephalyHumansIn Situ Hybridization, FluorescenceInfantInfant, NewbornKaryotypingMaleMosaicismPolymerase Chain ReactionProteinsTrans-ActivatorsTranslocation, GeneticConceptsSonic hedgehog geneHedgehog geneCell linesDifferent chromosome regionsStructural rearrangementsHuman Sonic Hedgehog genePatient cell linesMolecular cytogenetic analysisChromosome regionsChromosome 7Embryonic forebrainLong armGenesSitu hybridizationPolymerase chain reaction amplificationHPE3Chain reaction amplificationCytogenetic analysisMidline cleavageReaction amplificationRearrangementPhenotypeCritical regionLinesHybridization
1995
The human A1 adenosine receptor: ligand binding properties, sites of somatic expression and chromosomal localization
Rivkees S, Lasbury M, Stiles G, Henegariu O, Curtis C, Vance G. The human A1 adenosine receptor: ligand binding properties, sites of somatic expression and chromosomal localization. Endocrine 1995, 3: 623-629. PMID: 21153220, DOI: 10.1007/bf02746338.Peer-Reviewed Original ResearchA1AR geneHuman-rodent somatic cell hybridsChromosome 1Human A1ARHuman chromosome 1Somatic cell hybridsLigand binding propertiesChromosomal localizationChromosomal locationMammalian biologyCell hybridsGene expressionNorthern analysisGenesCHO cellsImportant biological effectsLong armPCR analysisSouthern blottingSitu hybridizationHuman A1 adenosine receptorSomatic expressionHigh-affinity receptorHuman physiologyHuman tissues