Improvements in cytogenetic slide preparation: Controlled chromosome spreading, chemical aging and gradual denaturing
Henegariu O, Heerema N, Wright L, Bray‐Ward P, Ward D, Vance G. Improvements in cytogenetic slide preparation: Controlled chromosome spreading, chemical aging and gradual denaturing. Cytometry 2001, 43: 101-109. PMID: 11169574, DOI: 10.1002/1097-0320(20010201)43:2<101::aid-cyto1024>3.0.co;2-8.Peer-Reviewed Original ResearchConceptsComparative genomic hybridizationClassical banding techniquesSitu hybridization applicationsChromosomal integrityChromosome spreadsMultiplex FISHMolecular cytogeneticsFISH applicationsChromosome spreadingFISH techniqueGenomic hybridizationEnvironmental conditionsSitu hybridizationBanding techniquesChromosomesHybridizationReproducible protocolFISH resultsCytogenetic slidesCell suspensionsSlide preparationFishMetaphase