2024
Optimizing Visualization of Axonal Transport of Endogenous Cargo by Fluorescence Microscopy in Living Caenorhabditis elegans.
Glomb O, Lyu M, Yogev S. Optimizing Visualization of Axonal Transport of Endogenous Cargo by Fluorescence Microscopy in Living Caenorhabditis elegans. Journal Of Visualized Experiments 2024 PMID: 38436410, DOI: 10.3791/66236.Peer-Reviewed Original ResearchConceptsSynaptic vesicle precursorsCaenorhabditis elegansAxonal cargosLoss of axonal transportCRISPR-Cas9 genome editingAxonal transportImpairs neuronal growthNeuronal cell biologySite of synthesisCas9 genome editingRAB-3Vesicle precursorsC. elegansGenome editingEndogenous labelingEndogenous cargoAxonal proteinsLiving Caenorhabditis elegansCell biologyCytoplasmic backgroundFluorescence microscopyCargoNeuronal growthNeuronal cell bodiesCaenorhabditis
2010
Polarized Secretion of Drosophila EGFR Ligand from Photoreceptor Neurons Is Controlled by ER Localization of the Ligand-Processing Machinery
Yogev S, Schejter ED, Shilo BZ. Polarized Secretion of Drosophila EGFR Ligand from Photoreceptor Neurons Is Controlled by ER Localization of the Ligand-Processing Machinery. PLOS Biology 2010, 8: e1000505. PMID: 20957186, PMCID: PMC2950126, DOI: 10.1371/journal.pbio.1000505.Peer-Reviewed Original ResearchConceptsRhomboid 3Photoreceptor neuronsEndoplasmic reticulum localizationAxonal terminiDrosophila visual systemRhomboid proteasesLigand SpitzTrafficking stepsER localizationSubcellular localizationPolarized secretionEGFR ligandsPhotoreceptor axonsCell bodiesTerminusLocalizationNeuronal cell bodiesSecretionTraffickingMachineryProteinERNeuronsProteaseAxons