2021
Preservation of vision after CaMKII-mediated protection of retinal ganglion cells
Guo X, Zhou J, Starr C, Mohns EJ, Li Y, Chen EP, Yoon Y, Kellner CP, Tanaka K, Wang H, Liu W, Pasquale LR, Demb JB, Crair MC, Chen B. Preservation of vision after CaMKII-mediated protection of retinal ganglion cells. Cell 2021, 184: 4299-4314.e12. PMID: 34297923, PMCID: PMC8530265, DOI: 10.1016/j.cell.2021.06.031.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAxonsBrainCalcium-Calmodulin-Dependent Protein Kinase Type 2Cyclic AMP Response Element-Binding ProteinCytoprotectionDependovirusDisease Models, AnimalEnzyme ActivationGlaucomaMice, Inbred C57BLNeurotoxinsOptic Nerve InjuriesRetinal Ganglion CellsSignal TransductionVision, OcularConceptsRetinal ganglion cellsRGC survivalRGC somataGanglion cellsDiverse insultsRGC axon projectionOptic nerve injurySole output neuronsPreservation of visionElevated intraocular pressureIrreversible vision lossPathological statesExcitotoxic injuryNerve injuryGlaucoma modelIntraocular pressureRGC axonsVision lossVisual functionNormal retinaVisual cortexAxon projectionsGenetic deficiencyInjuryRetinaPhotoreceptive Ganglion Cells Drive Circuits for Local Inhibition in the Mouse Retina
Pottackal J, Walsh HL, Rahmani P, Zhang K, Justice NJ, Demb JB. Photoreceptive Ganglion Cells Drive Circuits for Local Inhibition in the Mouse Retina. Journal Of Neuroscience 2021, 41: 1489-1504. PMID: 33397711, PMCID: PMC7896016, DOI: 10.1523/jneurosci.0674-20.2020.Peer-Reviewed Original ResearchMeSH KeywordsAmacrine CellsAnimalsCorticotropin-Releasing HormoneElectrophysiological PhenomenaExcitatory Postsynaptic PotentialsFemaleGamma-Aminobutyric AcidGap JunctionsMaleMiceMice, Inbred C57BLNeural InhibitionNeuronsOptogeneticsPhotoreceptor Cells, VertebrateRetinaRetinal Cone Photoreceptor CellsRetinal Ganglion CellsRetinal Rod Photoreceptor CellsRod OpsinsSynapsesConceptsGap junction-mediated electrical synapsesAmacrine cellsElectrical synapsesIpRGC activityGanglion cellsRetinal interneuronsRetinal circuitsPhotosensitive retinal ganglion cellsGABAergic amacrine cellsRetinal ganglion cellsWhole-cell recordingsSpecific RGC typesAbsence of rodsIpRGC typesRGC typesPharmacological blockadeRetinal neuronsMelanopsin expressionMature retinaMouse retinaSynaptic circuitsNeuronal circuitsInterneuronsOptogenetic stimulationLocal inhibition
2020
Connectomic analysis reveals an interneuron with an integral role in the retinal circuit for night vision
Park SJ, Lieberman EE, Ke JB, Rho N, Ghorbani P, Rahmani P, Jun NY, Lee HL, Kim IJ, Briggman KL, Demb JB, Singer JH. Connectomic analysis reveals an interneuron with an integral role in the retinal circuit for night vision. ELife 2020, 9: e56077. PMID: 32412412, PMCID: PMC7228767, DOI: 10.7554/elife.56077.Peer-Reviewed Original ResearchConceptsAII amacrine cellsProjection neuronsSingle interneuron typeConnectomic analysisBipolar cell pathwaysNovel neural circuitGABAergic cellsAmacrine cellsCentral neuronsGanglion cellsSynaptic layersRetinal circuitsInterneuron typesSurround inhibitionMouse retinaNight visionON responseExcitatory centerOptogenetic analysesNeural circuitsInhibitory surroundCell pathwaysRod photoreceptorsReceptive fieldsRb pathway
2018
Convergence and Divergence of CRH Amacrine Cells in Mouse Retinal Circuitry
Park SJH, Pottackal J, Ke JB, Jun NY, Rahmani P, Kim IJ, Singer JH, Demb JB. Convergence and Divergence of CRH Amacrine Cells in Mouse Retinal Circuitry. Journal Of Neuroscience 2018, 38: 3753-3766. PMID: 29572434, PMCID: PMC5895998, DOI: 10.1523/jneurosci.2518-17.2018.Peer-Reviewed Original ResearchConceptsAlpha ganglion cellsGanglion cell typesAmacrine cellsGanglion cellsCRH cellsAlpha cellsGABAergic synapsesInhibitory interneuronsExcitation/inhibition balanceCorticotropin-releasing hormoneCre transgenic miceFire action potentialsTonic excitatory driveCell typesBalance of excitationGABA releaseExcitatory circuitsRetinal circuitryExcitatory driveInhibition balanceInhibitory inputsMammalian retinaMouse retinaAction potentialsOptogenetic analyses
2016
Complexin 3 Increases the Fidelity of Signaling in a Retinal Circuit by Regulating Exocytosis at Ribbon Synapses
Mortensen LS, Park SJ, Ke JB, Cooper BH, Zhang L, Imig C, Löwel S, Reim K, Brose N, Demb JB, Rhee JS, Singer JH. Complexin 3 Increases the Fidelity of Signaling in a Retinal Circuit by Regulating Exocytosis at Ribbon Synapses. Cell Reports 2016, 15: 2239-2250. PMID: 27239031, PMCID: PMC5134263, DOI: 10.1016/j.celrep.2016.05.012.Peer-Reviewed Original ResearchConceptsRod bipolarsAsynchronous releaseAmacrine cell synapsesRetinal ganglion cellsRetinal pathwaysGanglion cellsCell synapsesRetinal circuitsRibbon synapsesMouse retinaMultivesicular releaseNeural circuitsComplexin proteinsSynapsesCircuit functionCplx3SignalingStudy linksReleaseExocytosisRB outputRetina
2015
Function and Circuitry of VIP+ Interneurons in the Mouse Retina
Park SJ, Borghuis BG, Rahmani P, Zeng Q, Kim IJ, Demb JB. Function and Circuitry of VIP+ Interneurons in the Mouse Retina. Journal Of Neuroscience 2015, 35: 10685-10700. PMID: 26224854, PMCID: PMC4518048, DOI: 10.1523/jneurosci.0222-15.2015.Peer-Reviewed Original ResearchConceptsGanglion cell layerAmacrine cell typesInner nuclear layerAmacrine cellsGanglion cellsINL cellsON pathwayGanglion cell dendritesGanglion cell typesBipolar cell terminalsWhole-cell recordingsCell typesNervous system functionSpatial tuningBipolar cell pathwaysTransgenic mouse lineGCL cellsOptic nerveGABAergic interneuronsRetinal functionCell dendritesRetinal circuitryInhibitory interneuronsNuclear layerBipolar interneurons
2014
Kainate Receptors Mediate Signaling in Both Transient and Sustained OFF Bipolar Cell Pathways in Mouse Retina
Borghuis BG, Looger LL, Tomita S, Demb JB. Kainate Receptors Mediate Signaling in Both Transient and Sustained OFF Bipolar Cell Pathways in Mouse Retina. Journal Of Neuroscience 2014, 34: 6128-6139. PMID: 24790183, PMCID: PMC4004803, DOI: 10.1523/jneurosci.4941-13.2014.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsDrug InteractionsExcitatory Amino Acid AntagonistsFemaleGlutamic AcidHexamethoniumIn Vitro TechniquesLightMaleMiceMice, Inbred C57BLNicotinic AntagonistsPatch-Clamp TechniquesPhotic StimulationPropionatesReceptors, Kainic AcidRetinaRetinal Bipolar CellsSignal TransductionVisual PathwaysConceptsGlutamate releaseBipolar cellsCone bipolar cell typesOFF bipolar cell pathwaysMouse bipolar cellsGanglion cell dendritesKainate-type glutamate receptorsBipolar cell typesLight-evoked responsesBipolar cell pathwaysGanglion cellsOFF layerCell dendritesReceptor mediatesGlutamate receptorsKainate receptorsAMPA receptorsTwo-photon imagingMouse retinaBipolar pathwayIntact circuitsOFF pathwaysElectrophysiological recordingsCircuit mechanismsReceptorsExcitatory Synaptic Inputs to Mouse On-Off Direction-Selective Retinal Ganglion Cells Lack Direction Tuning
Park SJ, Kim IJ, Looger LL, Demb JB, Borghuis BG. Excitatory Synaptic Inputs to Mouse On-Off Direction-Selective Retinal Ganglion Cells Lack Direction Tuning. Journal Of Neuroscience 2014, 34: 3976-3981. PMID: 24623775, PMCID: PMC3951696, DOI: 10.1523/jneurosci.5017-13.2014.Peer-Reviewed Original ResearchConceptsDirection-selective ganglion cellsOFF direction-selective ganglion cellsStarburst amacrine cellsGlutamate releaseBipolar cellsExcitatory currentsPreferred direction motionBipolar cell typesExcitatory synaptic inputsNull-direction inhibitionGABA-A receptorsWhole-cell recordingsNull-direction motionDSGC dendritesGABA releaseCholinergic inputAmacrine cellsGanglion cellsSynaptic mechanismsSynaptic inputsMammalian retinaMouse retinaIntensity-based glutamate-sensing fluorescent reporterDirection selectivityDirection tuningDevelopmental Changes in NMDA Receptor Subunit Composition at ON and OFF Bipolar Cell Synapses onto Direction-Selective Retinal Ganglion Cells
Stafford BK, Park SJ, Wong KY, Demb JB. Developmental Changes in NMDA Receptor Subunit Composition at ON and OFF Bipolar Cell Synapses onto Direction-Selective Retinal Ganglion Cells. Journal Of Neuroscience 2014, 34: 1942-1948. PMID: 24478373, PMCID: PMC3905152, DOI: 10.1523/jneurosci.4461-13.2014.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnimalsAnimals, NewbornExcitatory Amino Acid AgonistsFemaleGene Expression Regulation, DevelopmentalGlutamic AcidGreen Fluorescent ProteinsIn Vitro TechniquesLightMaleMembrane PotentialsMiceMice, Inbred C57BLMice, TransgenicN-MethylaspartateNeurotransmitter AgentsPatch-Clamp TechniquesReceptors, N-Methyl-D-AspartateRetinaRetinal Bipolar CellsRetinal Ganglion CellsSynapsesVisual PathwaysConceptsNMDA-type glutamate receptorsDirection-selective ganglion cellsExtrasynaptic NMDA-type glutamate receptorsNMDAR-mediated responsesBipolar cell synapsesGanglion cellsCell synapsesMature retinaSpecific ganglion cell typesDirection-selective retinal ganglion cellsNMDA receptor subunit compositionActivity-dependent synaptic developmentBipolar cell inputsGanglion cell typesRetinal ganglion cellsFirst postnatal monthReceptor subunit compositionWhole-cell recordingsGlutamatergic synapse formationCell typesGluN2B antagonist ifenprodilReceptor blockadeGlutamate releaseBipolar synapsesGlutamatergic synapses
2013
Adaptation to Background Light Enables Contrast Coding at Rod Bipolar Cell Synapses
Ke JB, Wang YV, Borghuis BG, Cembrowski MS, Riecke H, Kath WL, Demb JB, Singer JH. Adaptation to Background Light Enables Contrast Coding at Rod Bipolar Cell Synapses. Neuron 2013, 81: 388-401. PMID: 24373883, PMCID: PMC4267681, DOI: 10.1016/j.neuron.2013.10.054.Peer-Reviewed Original ResearchMeSH Keywords2-Amino-5-phosphonovalerateAdaptation, OcularAnimalsBiophysicsComputer SimulationExcitatory Amino Acid AntagonistsGlucosamine 6-Phosphate N-AcetyltransferaseGreen Fluorescent ProteinsIn Vitro TechniquesLightMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicModels, NeurologicalPatch-Clamp TechniquesQuinoxalinesRetinal Bipolar CellsRetinal Rod Photoreceptor CellsSynapsesVisual PathwaysTwo-Photon Imaging of Nonlinear Glutamate Release Dynamics at Bipolar Cell Synapses in the Mouse Retina
Borghuis BG, Marvin JS, Looger LL, Demb JB. Two-Photon Imaging of Nonlinear Glutamate Release Dynamics at Bipolar Cell Synapses in the Mouse Retina. Journal Of Neuroscience 2013, 33: 10972-10985. PMID: 23825403, PMCID: PMC3718381, DOI: 10.1523/jneurosci.1241-13.2013.Peer-Reviewed Original ResearchConceptsInner plexiform layerGanglion cellsPlexiform layerY-type retinal ganglion cellsY-type ganglion cellsPresynaptic bipolar cellsBipolar cell outputBipolar cell synapsesRetinal ganglion cellsWhole-cell recordingsPostsynaptic ganglion cellsIntact neural circuitsReceptive field propertiesPresynaptic release sitesBipolar terminalsGlutamate releaseGlutamate synapsesSynaptic basisCell synapsesBipolar cellsExcitatory currentsSurround inhibitionMouse retinaTwo-photon imagingIndependent release
2012
Form and Function of the M4 Cell, an Intrinsically Photosensitive Retinal Ganglion Cell Type Contributing to Geniculocortical Vision
Estevez ME, Fogerson PM, Ilardi MC, Borghuis BG, Chan E, Weng S, Auferkorte ON, Demb JB, Berson DM. Form and Function of the M4 Cell, an Intrinsically Photosensitive Retinal Ganglion Cell Type Contributing to Geniculocortical Vision. Journal Of Neuroscience 2012, 32: 13608-13620. PMID: 23015450, PMCID: PMC3474539, DOI: 10.1523/jneurosci.1422-12.2012.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBeta-GalactosidaseCholera ToxinCholine O-AcetyltransferaseDendritesElectroretinographyFemaleGeniculate BodiesGreen Fluorescent ProteinsLightMaleMembrane PotentialsMiceMice, Inbred C57BLMice, TransgenicPatch-Clamp TechniquesPhotic StimulationRetinaRetinal Ganglion CellsRod OpsinsVisual CortexVisual FieldsVisual PathwaysConceptsM4 cellsM2 cellsDorsal lateral geniculate nucleusPhotosensitive retinal ganglion cellsInner plexiform layerRetinal ganglion cellsLateral geniculate nucleusRetinal output neuronsNonlinear spatial summationCholinergic bandDendritic stratificationON sublaminaLarge somataPlexiform layerGanglion cellsDendritic arborsGeniculate nucleusPhotopigment melanopsinVentral retinaDorsal retinaMouse retinaDirection selectivityFunctional rodsCentral projectionsM3 cells
2011
A Synaptic Mechanism for Retinal Adaptation to Luminance and Contrast
Jarsky T, Cembrowski M, Logan SM, Kath WL, Riecke H, Demb JB, Singer JH. A Synaptic Mechanism for Retinal Adaptation to Luminance and Contrast. Journal Of Neuroscience 2011, 31: 11003-11015. PMID: 21795549, PMCID: PMC3152984, DOI: 10.1523/jneurosci.2631-11.2011.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalAmacrine CellsAnimalsBiophysical PhenomenaBiophysicsCalciumContrast SensitivityElectric StimulationExcitatory Postsynaptic PotentialsFemaleIn Vitro TechniquesLightingMaleMiceMice, Inbred C57BLModels, NeurologicalNumerical Analysis, Computer-AssistedPatch-Clamp TechniquesPhotic StimulationPresynaptic TerminalsRetinaRetinal Bipolar CellsSynaptic TransmissionConceptsSynaptic mechanismsSynaptic transmissionBipolar cell synaptic transmissionMouse retinal slice preparationVesicle depletionCommon synaptic mechanismsPrimary sensory circuitsUse-dependent regulationAII amacrine cellsContrast adaptationRetinal slice preparationBipolar cell synapsesCell synaptic transmissionRetinal neural circuitWhole-cell recordingsRetinal bipolar cellsPhasic transmissionTonic transmissionAmacrine cellsSlice preparationCell synapsesBipolar cellsSame cellular mechanismsSensory circuitsNeural circuitsSpectral and Temporal Sensitivity of Cone-Mediated Responses in Mouse Retinal Ganglion Cells
Wang YV, Weick M, Demb JB. Spectral and Temporal Sensitivity of Cone-Mediated Responses in Mouse Retinal Ganglion Cells. Journal Of Neuroscience 2011, 31: 7670-7681. PMID: 21613480, PMCID: PMC3122925, DOI: 10.1523/jneurosci.0629-11.2011.Peer-Reviewed Original ResearchConceptsCone-mediated responsesM-opsin expressionGanglion cellsMouse retinaMouse retinal ganglion cellsRod-mediated responsesSlower temporal processingRetinal ganglion cellsWild-type miceGanglion cell responsesCone-mediated visionMouse ganglion cellsVentral retinaDorsal retinaRod functionCone functionCell responsesS-opsinRetinaMiceMouse photoreceptorsExpression ratioLight stimulationPhotoreceptor typesTemporal processing
2010
NMDA Receptor Contributions to Visual Contrast Coding
Manookin MB, Weick M, Stafford BK, Demb JB. NMDA Receptor Contributions to Visual Contrast Coding. Neuron 2010, 67: 280-293. PMID: 20670835, PMCID: PMC2913150, DOI: 10.1016/j.neuron.2010.06.020.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsContrast SensitivityDizocilpine MaleateElectric ConductivityExcitatory Amino Acid AgonistsExcitatory Amino Acid AntagonistsGene Expression RegulationGuinea PigsIn Vitro TechniquesLightMiceMice, Inbred C57BLN-MethylaspartatePatch-Clamp TechniquesPhotic StimulationPiperidinesReceptors, N-Methyl-D-AspartateRetinaRetinal Ganglion CellsVisual PerceptionConceptsNMDA receptorsOFF alphaAlpha cellsNMDA-type glutamate receptorsOFF alpha cellsDelta cellsGanglion cell typesNMDA receptor contributionCell typesExcitatory responsesGlutamate receptorsInhibitory currentsAMPA receptorsReceptor contributionGluN2B subunitContrast sensitivityContrast codingReceptorsNMDAAMPACertain cell typesCellsVisual processingAlpha