2011
Lateral assembly of the immunoglobulin protein SynCAM 1 controls its adhesive function and instructs synapse formation
Fogel AI, Stagi M, Perez de Arce K, Biederer T. Lateral assembly of the immunoglobulin protein SynCAM 1 controls its adhesive function and instructs synapse formation. The EMBO Journal 2011, 30: 4728-4738. PMID: 21926970, PMCID: PMC3243608, DOI: 10.1038/emboj.2011.336.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell AdhesionCell Adhesion Molecule-1Cell Adhesion MoleculesCell Adhesion Molecules, NeuronalCell DifferentiationCells, CulturedChlorocebus aethiopsCoculture TechniquesCOS CellsFluorescence Resonance Energy TransferHEK293 CellsHippocampusHumansImmunoglobulinsImmunohistochemistryMiceNeuritesProtein Structure, QuaternarySynapsesConceptsSynCAM 1Specialized adhesion sitesSynapse formationTrans-synaptic interactionsSynaptic cleftCI assemblyProtein complexesSynaptic cell adhesion molecule SynCAM 1Adhesion sitesSynaptogenic activityAdhesive functionSynapse developmentStructural organizationNovel insightsSynapse inductionLateral assemblyAdhesive capacityAdhesion moleculesSynaptic morphologyAdhesive mechanismsOligomerizationAssemblyAxo-dendritic contactsCleftLater stages
2007
Mixed-culture assays for analyzing neuronal synapse formation
Biederer T, Scheiffele P. Mixed-culture assays for analyzing neuronal synapse formation. Nature Protocols 2007, 2: 670-676. PMID: 17406629, DOI: 10.1038/nprot.2007.92.Peer-Reviewed Original ResearchConceptsVertebrate central nervous systemIndividual cell surface proteinsSynaptic adhesion complexesCell biological mechanismsPostsynaptic membrane domainsCell surface proteinsAssembly of synapsesAdhesion complexesMembrane domainsNeuronal synapse formationIndividual proteinsSynaptogenic activityGenetic studiesBidirectional signalingPrimary neuronal culturesSynapse formationSynaptic differentiationMixed culture assayBiological mechanismsSynaptic structureProteinNew insightsSynaptic cleftNeuronal culturesAssay system