2007
Mixed-culture assays for analyzing neuronal synapse formation
Biederer T, Scheiffele P. Mixed-culture assays for analyzing neuronal synapse formation. Nature Protocols 2007, 2: 670-676. PMID: 17406629, DOI: 10.1038/nprot.2007.92.Peer-Reviewed Original ResearchConceptsVertebrate central nervous systemIndividual cell surface proteinsSynaptic adhesion complexesCell biological mechanismsPostsynaptic membrane domainsCell surface proteinsAssembly of synapsesAdhesion complexesMembrane domainsNeuronal synapse formationIndividual proteinsSynaptogenic activityGenetic studiesBidirectional signalingPrimary neuronal culturesSynapse formationSynaptic differentiationMixed culture assayBiological mechanismsSynaptic structureProteinNew insightsSynaptic cleftNeuronal culturesAssay system
2001
CASK and Protein 4.1 Support F-actin Nucleation on Neurexins*
Biederer T, Südhof T. CASK and Protein 4.1 Support F-actin Nucleation on Neurexins*. Journal Of Biological Chemistry 2001, 276: 47869-47876. PMID: 11604393, DOI: 10.1074/jbc.m105287200.Peer-Reviewed Original ResearchMeSH KeywordsActinsAmino Acid SequenceAnimalsBinding SitesCalcium-Calmodulin-Dependent Protein KinasesCytoplasmCytoskeletal ProteinsGlycophorinsGuanylate KinasesMembrane ProteinsMolecular Sequence DataNerve Tissue ProteinsNeuropeptidesNucleoside-Phosphate KinaseProtein BindingRatsSequence Homology, Amino AcidSpectrinConceptsProtein 4.1Actin cytoskeletonActin filamentsBrain-enriched isoformF-actin nucleationCytosolic C-terminusClass of proteinsCell surface proteinsCytosolic tailPDZ domainAdaptor proteinCellular processesMembrane organizationC-terminusNeurexinsProtein p55Glycophorin CCell surfaceRat brain extractsIntercellular junctionsSpectrin filamentsErythrocyte isoformsCytoskeletonProteinLocomotion of cells
2000
Mints as Adaptors DIRECT BINDING TO NEUREXINS AND RECRUITMENT OF Munc18*
Biederer T, Südhof T. Mints as Adaptors DIRECT BINDING TO NEUREXINS AND RECRUITMENT OF Munc18*. Journal Of Biological Chemistry 2000, 275: 39803-39806. PMID: 11036064, DOI: 10.1074/jbc.c000656200.Peer-Reviewed Original ResearchConceptsAdaptor proteinPDZ domain-mediated interactionsDomain-mediated interactionsSynaptic vesicle exocytosisCell surface proteinsNeuronal adaptor proteinMultiprotein complexesProtein essentialCytoplasmic tailMunc18-1Vesicle exocytosisPlasma membraneMunc18NeurexinsDirect bindingPossible functionsSurface proteinsProteinExocytosisMINT1RecruitmentComplexesMint2BindsMint