2021
A Primed Subpopulation of Bacteria Enables Rapid Expression of the Type 3 Secretion System in Pseudomonas aeruginosa
Lin CK, Lee DSW, McKeithen-Mead S, Emonet T, Kazmierczak B. A Primed Subpopulation of Bacteria Enables Rapid Expression of the Type 3 Secretion System in Pseudomonas aeruginosa. MBio 2021, 12: 10.1128/mbio.00831-21. PMID: 34154400, PMCID: PMC8262847, DOI: 10.1128/mbio.00831-21.Peer-Reviewed Original ResearchConceptsType 3 secretion systemSecretion systemT3SS expressionVirulence traitsSpecific virulence traitsHuman disease severityComplex nanomachinesT3SS genesP. aeruginosa cellsReproductive fitnessIsogenic cellsHeterogeneous expressionCell envelopeT3SS effectorsMotility organellesReservoir of cellsCritical virulence traitsGene expressionRegulatory mechanismsSubpopulation of cellsGram-negative pathogensFluorescent reportersDivision timeP. aeruginosaPseudomonas aeruginosa
2010
Single‐cell quantification of IL‐2 response by effector and regulatory T cells reveals critical plasticity in immune response
Feinerman O, Jentsch G, Tkach KE, Coward JW, Hathorn MM, Sneddon MW, Emonet T, Smith KA, Altan-Bonnet G. Single‐cell quantification of IL‐2 response by effector and regulatory T cells reveals critical plasticity in immune response. Molecular Systems Biology 2010, 6: msb201090. PMID: 21119631, PMCID: PMC3010113, DOI: 10.1038/msb.2010.90.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedCoculture TechniquesEnzyme-Linked Immunosorbent AssayImmunity, CellularInterleukin-2Interleukin-2 Receptor alpha SubunitMiceMice, Inbred C57BLMice, TransgenicModels, BiologicalSingle-Cell AnalysisStaining and LabelingT-Lymphocytes, Helper-InducerT-Lymphocytes, RegulatoryConceptsInterleukin-2T cellsImmune responseSingle-cell quantificationSingle-cell measurementsEffector T cellsRegulatory T cellsIL-2 responseSurvival signalsReceptor expression levelsIndividual cellsSuppressive capacityCytokine regulationExpression levelsImmune systemHeterogeneous cellsSpecific suppressionSilico modelingPopulation levelCellsEffectorsResponseCritical plasticityPopulationSuppression