2023
Impaired Ghrelin Signaling Does Not Lead to Alterations of Anxiety-like Behaviors in Adult Mice Chronically Exposed to THC during Adolescence
Sestan-Pesa M, Shanabrough M, Horvath T, Miletta M. Impaired Ghrelin Signaling Does Not Lead to Alterations of Anxiety-like Behaviors in Adult Mice Chronically Exposed to THC during Adolescence. Biomedicines 2023, 11: 144. PMID: 36672651, PMCID: PMC9855766, DOI: 10.3390/biomedicines11010144.Peer-Reviewed Original ResearchAnxiety-like behaviorGrowth hormone secretagogue receptorTHC exposureAdult miceGHSR knockout miceDepression-like behaviorLate adolescenceWild-type littermatesLong-term useGHSR signalingGhrelin signalingSecretagogue receptorKnockout miceAnimal modelsTetrahydrocannabinol (THC) administrationMental illnessMiceSignificant alterationsGhrelinMarijuana useAdolescenceReceptorsProlonged periodExposureLong-term impact
2018
Neuronal Cilia: Another Player in the Melanocortin System
Varela L, Horvath TL. Neuronal Cilia: Another Player in the Melanocortin System. Trends In Molecular Medicine 2018, 24: 333-334. PMID: 29501261, DOI: 10.1016/j.molmed.2018.02.004.Peer-Reviewed Original Research
2017
Fetal Growth Restriction Caused by Sexual Transmission of Zika Virus in Mice
Uraki R, Jurado KA, Hwang J, Szigeti-Buck K, Horvath TL, Iwasaki A, Fikrig E. Fetal Growth Restriction Caused by Sexual Transmission of Zika Virus in Mice. The Journal Of Infectious Diseases 2017, 215: 1720-1724. PMID: 28472297, PMCID: PMC5853330, DOI: 10.1093/infdis/jix204.Peer-Reviewed Original ResearchConceptsZika virusSexual transmissionWeight of fetusesFetal growth restrictionNaive female miceType I interferon receptorEmbryonic day 18.5Female miceGrowth restrictionMale miceOcular deformityMosquito bitesControl groupDay 18.5Fetal abnormalitiesSexual contactInterferon receptorMiceFetusesInfected malesVirusDeformityAbnormalitiesReceptors
2014
Leptin signaling in astrocytes regulates hypothalamic neuronal circuits and feeding
Kim JG, Suyama S, Koch M, Jin S, Argente-Arizon P, Argente J, Liu ZW, Zimmer MR, Jeong JK, Szigeti-Buck K, Gao Y, Garcia-Caceres C, Yi CX, Salmaso N, Vaccarino FM, Chowen J, Diano S, Dietrich MO, Tschöp MH, Horvath TL. Leptin signaling in astrocytes regulates hypothalamic neuronal circuits and feeding. Nature Neuroscience 2014, 17: 908-910. PMID: 24880214, PMCID: PMC4113214, DOI: 10.1038/nn.3725.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAstrocytesCell CountEatingExcitatory Postsynaptic PotentialsGlial Fibrillary Acidic ProteinHypothalamusImmunohistochemistryIn Situ HybridizationLeptinMaleMelanocortinsMiceMice, KnockoutMicroscopy, ElectronNerve NetNeuronsPrimary Cell CulturePro-OpiomelanocortinPulmonary Gas ExchangeReal-Time Polymerase Chain ReactionRNA, MessengerSignal Transduction
2013
Intranasal epidermal growth factor treatment rescues neonatal brain injury
Scafidi J, Hammond TR, Scafidi S, Ritter J, Jablonska B, Roncal M, Szigeti-Buck K, Coman D, Huang Y, McCarter RJ, Hyder F, Horvath TL, Gallo V. Intranasal epidermal growth factor treatment rescues neonatal brain injury. Nature 2013, 506: 230-234. PMID: 24390343, PMCID: PMC4106485, DOI: 10.1038/nature12880.Peer-Reviewed Original ResearchMeSH KeywordsAdministration, IntranasalAnimalsAnimals, NewbornBrain InjuriesCell DifferentiationCell DivisionCell LineageCell SurvivalDemyelinating DiseasesDisease Models, AnimalEpidermal Growth FactorErbB ReceptorsHumansHypoxiaInfant, Premature, DiseasesMaleMiceMolecular Targeted TherapyOligodendrogliaRegenerationSignal TransductionStem CellsTime FactorsConceptsDiffuse white matter injuryNeonatal brain injuryVery preterm infantsWhite matter injuryOligodendrocyte precursor cellsEpidermal growth factor receptorGrowth factor treatmentGrowth factor receptorPreterm infantsFunctional recoveryBrain injurySuch injuriesEpidermal growth factor treatmentMouse modelFactor treatmentInjuryFactor receptorPrecursor cellsInfantsReceptorsAntibodies to cannabinoid type 1 receptor co‐react with stomatin‐like protein 2 in mouse brain mitochondria
Morozov YM, Dominguez MH, Varela L, Shanabrough M, Koch M, Horvath TL, Rakic P. Antibodies to cannabinoid type 1 receptor co‐react with stomatin‐like protein 2 in mouse brain mitochondria. European Journal Of Neuroscience 2013, 38: 2341-2348. PMID: 23617247, PMCID: PMC3902808, DOI: 10.1111/ejn.12237.Peer-Reviewed Original ResearchConceptsStomatin-like protein 2Type 1 receptorPresence of CB1Protein 2Anti-CB1 antibodySynthetic cannabinoid WINMouse brain mitochondriaCerebral cortexEndocannabinoid signalingBrain cellsCannabinoid WINNeuronal mitochondriaBrain mitochondriaAntibodiesMitochondrial functionCB1Polyclonal antibodiesCortexMitochondrial preparationsSerumReceptors
2012
A marriage made to last in drug design
Dietrich MO, Horvath TL. A marriage made to last in drug design. Nature Medicine 2012, 18: 1737-1738. PMID: 23223057, DOI: 10.1038/nm.3018.Peer-Reviewed Original Research
2011
Nicotine Decreases Food Intake Through Activation of POMC Neurons
Mineur YS, Abizaid A, Rao Y, Salas R, DiLeone RJ, Gündisch D, Diano S, De Biasi M, Horvath TL, Gao XB, Picciotto MR. Nicotine Decreases Food Intake Through Activation of POMC Neurons. Science 2011, 332: 1330-1332. PMID: 21659607, PMCID: PMC3113664, DOI: 10.1126/science.1201889.Peer-Reviewed Original ResearchConceptsFood intakePOMC neuronsNicotine decreases food intakeDecrease food intakePro-opiomelanocortin (POMC) neuronsΑ3β4 nicotinic acetylcholine receptorsHypothalamic melanocortin systemNicotine-induced decreasesMelanocortin-4 receptorNicotinic acetylcholine receptorsAnorexic effectDecrease appetiteSmoking cessationSynaptic mechanismsMelanocortin systemNovel treatmentsBody weightAcetylcholine receptorsNeurobiological mechanismsNeuronsIntakeSubsequent activationAppetiteActivationReceptors
2009
A Serotonin-Dependent Mechanism Explains the Leptin Regulation of Bone Mass, Appetite, and Energy Expenditure
Yadav VK, Oury F, Suda N, Liu ZW, Gao XB, Confavreux C, Klemenhagen KC, Tanaka KF, Gingrich JA, Guo XE, Tecott LH, Mann JJ, Hen R, Horvath TL, Karsenty G. A Serotonin-Dependent Mechanism Explains the Leptin Regulation of Bone Mass, Appetite, and Energy Expenditure. Cell 2009, 138: 976-989. PMID: 19737523, PMCID: PMC2768582, DOI: 10.1016/j.cell.2009.06.051.Peer-Reviewed Original ResearchConceptsSerotonergic neuronsHypothalamic neuronsBone massEnergy expenditureVentromedial hypothalamic neuronsBone mass accrualSerotonin-dependent mechanismRegulation of appetiteEnergy expenditure phenotypesSpecific hypothalamic neuronsHtr2c receptorLeptin deficiencyArcuate neuronsLeptin inhibitionSerotonin synthesisLeptin receptorLeptin regulationLeptinNeuronsAppetiteReceptorsEnergy metabolismBrainBoneMolecular basisLeptin Acts via Leptin Receptor-Expressing Lateral Hypothalamic Neurons to Modulate the Mesolimbic Dopamine System and Suppress Feeding
Leinninger GM, Jo YH, Leshan RL, Louis GW, Yang H, Barrera JG, Wilson H, Opland DM, Faouzi MA, Gong Y, Jones JC, Rhodes CJ, Chua S, Diano S, Horvath TL, Seeley RJ, Becker JB, Münzberg H, Myers MG. Leptin Acts via Leptin Receptor-Expressing Lateral Hypothalamic Neurons to Modulate the Mesolimbic Dopamine System and Suppress Feeding. Cell Metabolism 2009, 10: 89-98. PMID: 19656487, PMCID: PMC2723060, DOI: 10.1016/j.cmet.2009.06.011.Peer-Reviewed Original ResearchConceptsLateral hypothalamic areaVentral tegmental areaMesolimbic DA systemLepRb neuronsMesolimbic dopamine systemLeptin actionLeptin receptorDopamine systemDA systemLeptin-deficient animalsLateral hypothalamic neuronsAnorexigenic hormone leptinLeptin actsHypothalamic areaHypothalamic neuronsSuppress feedingHormone leptinTegmental areaDA contentInhibitory neuronsRate-limiting enzymeBody weightNeuronsLeptinReceptors
2006
Serotonin Reciprocally Regulates Melanocortin Neurons to Modulate Food Intake
Heisler LK, Jobst EE, Sutton GM, Zhou L, Borok E, Thornton-Jones Z, Liu HY, Zigman JM, Balthasar N, Kishi T, Lee CE, Aschkenasi CJ, Zhang CY, Yu J, Boss O, Mountjoy KG, Clifton PG, Lowell BB, Friedman JM, Horvath T, Butler AA, Elmquist JK, Cowley MA. Serotonin Reciprocally Regulates Melanocortin Neurons to Modulate Food Intake. Neuron 2006, 51: 239-249. PMID: 16846858, DOI: 10.1016/j.neuron.2006.06.004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsEatingElectric StimulationMaleMiceMice, Inbred AMice, Inbred C57BLMice, KnockoutMice, ObeseMice, TransgenicNerve NetNeuronsPyridinesReceptor, Melanocortin, Type 3Receptor, Melanocortin, Type 4Receptor, Serotonin, 5-HT1BReceptors, MelanocortinSerotoninSerotonin 5-HT1 Receptor AgonistsConceptsFood intakePeripheral adiposity signalsBody weight homeostasisCentral serotonergic systemMelanocortin receptor agonistModulates food intakeSerotonin1B receptorsMelanocortin neuronsWeight homeostasisMelanocortin-3Receptor agonistSerotonergic regulationAdiposity signalsSerotonergic systemEndogenous releaseMelanocortin-4Central circuitryBody weightNeural pathwaysMelanocortin receptorsReceptorsDownstream activationAgonistsAntagonistIntake
2004
CPG2 A brain- and synapse-specific protein that regulates the endocytosis of glutamate receptors
Cottrell JR, Borok E, Horvath TL, Nedivi E. CPG2 A brain- and synapse-specific protein that regulates the endocytosis of glutamate receptors. Neuron 2004, 44: 677-690. PMID: 15541315, PMCID: PMC3065105, DOI: 10.1016/j.neuron.2004.10.025.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBlotting, NorthernBlotting, WesternBrainCells, CulturedClathrin-Coated VesiclesEndocytosisHumansIn Situ HybridizationMicroscopy, ElectronMolecular Sequence DataNerve Tissue ProteinsNeuronal PlasticityNeuronsReceptors, AMPAReceptors, GlutamateReceptors, N-Methyl-D-AspartateReverse Transcriptase Polymerase Chain ReactionSynapsesConceptsGlutamate receptorsClathrin-coated vesiclesBrain-specific splice variantSynapse-specific proteinsExcitatory synapsesReceptor endocytosisSYNE-1 geneConstitutive internalizationEndocytic mechanismsSynaptic AMPA receptorsDendritic spine sizeMembrane transportSplice variantsSynaptic proteinsNMDA receptorsAMPA receptorsProteinPostsynaptic plasticityNeurotransmitter receptorsEndocytosisSynaptic strengthLong-term maintenanceReceptorsSpine sizeInternalization
2000
AMPA receptors colocalize with neuropeptide-Y- and galanin-containing, but not with dopamine, neurons of the female rat arcuate nucleus: a semiquantitative immunohistochemical colocalization study
Zsarnovszky A, Horvath T, Naftolin F, Leranth C. AMPA receptors colocalize with neuropeptide-Y- and galanin-containing, but not with dopamine, neurons of the female rat arcuate nucleus: a semiquantitative immunohistochemical colocalization study. Experimental Brain Research 2000, 133: 532-537. PMID: 10985687, DOI: 10.1007/s002210000425.Peer-Reviewed Original ResearchConceptsArcuate nucleusAMPA receptorsFemale rat arcuate nucleusExcitatory amino acid neurotransmissionIsoxazole propionic acid (AMPA) receptorsAmino acid neurotransmissionTH-ir cellsMajor neuronal populationsRat arcuate nucleusPropionic acid receptorsHormone delivery systemsImmunohistochemical colocalization studiesGlutamatergic innervationGlutamatergic inputsFemale ratsNeuronal populationsGalaninAcid receptorsNeuronsReceptorsNPYNeuropeptidesColocalization studiesPresent studyDelivery systemEvidence that NPY Y1 receptors are involved in stimulation of feeding by orexins (hypocretins) in sated rats
Jain M, Horvath T, Kalra P, Kalra S. Evidence that NPY Y1 receptors are involved in stimulation of feeding by orexins (hypocretins) in sated rats. Peptides 2000, 87: 19-24. PMID: 10710284, DOI: 10.1016/s0167-0115(99)00102-0.Peer-Reviewed Original ResearchConceptsNeuropeptide YLateral hypothalamic areaArcuate nucleusY1 receptorParaventricular nucleusSelective NPY Y1 receptor antagonistNPY Y1 receptor antagonistNPY Y1 receptorY1 receptor antagonistStimulation of feedingAdult male ratsDose-dependent mannerOrexigenic peptideOrexin ANPY receptorsExcitatory effectsHypothalamic areaHypothalamic appetiteReceptor antagonistMale ratsSated ratsOrexinNeural sitesReceptorsNPYergic
1999
Estrogen receptor-α in the raphe serotonergic and supramammillary area calretinin-containing neurons of the female rat
Leranth C, Shanabrough M, Horvath T. Estrogen receptor-α in the raphe serotonergic and supramammillary area calretinin-containing neurons of the female rat. Experimental Brain Research 1999, 128: 417-420. PMID: 10501815, DOI: 10.1007/s002210050863.Peer-Reviewed Original ResearchConceptsEstrogen receptorLong-term potentiationMedian rapheSupramammillary areaSubcortical structuresCalretinin-containing neuronsHippocampal long-term potentiationEffects of estrogenHippocampal electric activityCalretinin neuronsFemale ratsGonadal hormonesTheta rhythmRapheVibratome sectionsReceptorsNeuronsSerotoninElectric activityEstrogenRhythm regulationPotentiationLarge populationMemory processesCalretinin
1998
Leptin receptors in estrogen receptor-containing neurons of the female rat hypothalamus
Diano S, Kalra S, Sakamoto H, Horvath T. Leptin receptors in estrogen receptor-containing neurons of the female rat hypothalamus. Brain Research 1998, 812: 256-259. PMID: 9813356, DOI: 10.1016/s0006-8993(98)00936-6.Peer-Reviewed Original ResearchConceptsLeptin receptorEstrogen receptorNeuronal perikaryaPeripheral signalsEstrogen receptor-containing neuronsParvicellular paraventricular nucleusReceptor-containing neuronsMedial preoptic areaVentromedial hypothalamic nucleusFemale rat hypothalamusArcuate nucleusHypothalamic nucleiFemale ratsParaventricular nucleusPreoptic areaGonadal functionHypothalamic sectionsPeriventricular regionRat hypothalamusNeuroendocrine mechanismsReceptorsExtensive colocalizationLeptinHypothalamusPerikaryaKainate Glutamate Receptors (GluR5–7) in the Rat Arcuate Nucleus: Relationship to Tanycytes, Astrocytes, Neurons and Gonadal Steroid Receptors
Diano S, Naftolin F, Horvath T. Kainate Glutamate Receptors (GluR5–7) in the Rat Arcuate Nucleus: Relationship to Tanycytes, Astrocytes, Neurons and Gonadal Steroid Receptors. Journal Of Neuroendocrinology 1998, 10: 239-247. PMID: 9630393, DOI: 10.1046/j.1365-2826.1998.00195.x.Peer-Reviewed Original ResearchConceptsGonadal steroid receptorsKainate glutamate receptorsArcuate nucleusGlutamate receptorsKainate receptorsSteroid receptorsGlutamate actionAndrogen receptorGlial elementsMorphological synaptic plasticityNeuro-glial interactionsRat arcuate nucleusIonotropic glutamate receptorsElectron microscopic immunocytochemistrySame perikaryaGonadal steroidsExcitatory neurotransmissionSynaptic plasticityMicroscopic immunocytochemistryReceptorsNeuronsCell populationsDouble labelHormone regulationAstrocytes
1995
Galanin neurons exhibit estrogen receptor immunoreactivity in the female rat mediobasal hypothalamus
Horvath T, Leranth C, Kalra S, Naftolin F. Galanin neurons exhibit estrogen receptor immunoreactivity in the female rat mediobasal hypothalamus. Brain Research 1995, 675: 321-324. PMID: 7540929, DOI: 10.1016/0006-8993(94)01374-q.Peer-Reviewed Original ResearchConceptsPituitary hormone secretionEstrogen receptorHormone secretionMediobasal hypothalamusEstrogen receptor immunoreactivityHypothalamic luteinizing hormoneArcuate nucleus neuronsRat mediobasal hypothalamusPopulations of neuronsGalanin neuronsReceptor immunoreactivityOvarian steroidsEstrogen effectsNucleus neuronsLuteinizing hormoneFemale ratsHormone releaseGalaninHypothalamusNeuronsVibratome sectionsImmunoreactivityReceptorsHormoneSecretion