2024
Polarized localization of kinesin-1 and RIC-7 drives axonal mitochondria anterograde transport
Wu Y, Ding C, Sharif B, Weinreb A, Swaim G, Hao H, Yogev S, Watanabe S, Hammarlund M. Polarized localization of kinesin-1 and RIC-7 drives axonal mitochondria anterograde transport. Journal Of Cell Biology 2024, 223: e202305105. PMID: 38470363, PMCID: PMC10932739, DOI: 10.1083/jcb.202305105.Peer-Reviewed Original ResearchConceptsKinesin-1C. elegansN-terminal domainRetrograde trafficAnterograde trafficTransport mitochondriaMitochondria transportPolar localizationMiro-1CRISPR engineeringMitochondria localizationDisordered regionsMitochondriaTransport complexMitochondria distributionAxonal transportAnterograde transportAnterograde axonal transportMotor complexMiroAdaptorCRISPRGenes
2023
Structure-function analysis of ceTIR-1/hSARM1 explains the lack of Wallerian axonal degeneration in C. elegans
Khazma T, Grossman A, Guez-Haddad J, Feng C, Dabas H, Sain R, Weitman M, Zalk R, Isupov M, Hammarlund M, Hons M, Opatowsky Y. Structure-function analysis of ceTIR-1/hSARM1 explains the lack of Wallerian axonal degeneration in C. elegans. Cell Reports 2023, 42: 113026. PMID: 37635352, PMCID: PMC10675840, DOI: 10.1016/j.celrep.2023.113026.Peer-Reviewed Original ResearchConceptsC. elegansCryoelectron microscopy structureNematode C. elegansC. elegans neuronsStructure-function analysisMicroscopy structureNADase activityMolecular mechanismsElegansCellular NADModel animalsSpeciesAxon degenerationWallerian axonal degenerationOrthologsOctamerProteinSARM1DivergenceNADSARMExpressionActivityAxonal degeneration
2016
Axon regeneration in C. elegans: Worming our way to mechanisms of axon regeneration
Byrne AB, Hammarlund M. Axon regeneration in C. elegans: Worming our way to mechanisms of axon regeneration. Experimental Neurology 2016, 287: 300-309. PMID: 27569538, PMCID: PMC5136328, DOI: 10.1016/j.expneurol.2016.08.015.Peer-Reviewed Original ResearchConceptsC. elegansC. elegans researchC. elegans modelSimple nervous systemMammalian nervous systemConserved genomeElegansElegans modelRegeneration responseAxon regenerationCellular mechanismsRegeneration researchTransparent bodyNervous systemRegenerationGenomeFundamental questionsSpeciesMechanismTechnical advancesRegeneration studiesPotential future directions
2014
Axon regeneration in C. elegans
Hammarlund M, Jin Y. Axon regeneration in C. elegans. Current Opinion In Neurobiology 2014, 27: 199-207. PMID: 24794753, PMCID: PMC4122601, DOI: 10.1016/j.conb.2014.04.001.Peer-Reviewed Original ResearchAxon Regeneration Genes Identified by RNAi Screening in C. elegans
Nix P, Hammarlund M, Hauth L, Lachnit M, Jorgensen EM, Bastiani M. Axon Regeneration Genes Identified by RNAi Screening in C. elegans. Journal Of Neuroscience 2014, 34: 629-645. PMID: 24403161, PMCID: PMC3870940, DOI: 10.1523/jneurosci.3859-13.2014.Peer-Reviewed Original ResearchConceptsRNAi-based screenCandidate gene screenAnalysis of mutantsGrowth-inhibiting functionComplex molecular eventsCell-intrinsic factorsCaenorhabditis elegansC. elegansAxon regenerationGene candidatesRNAi screeningMembrane dynamicsMAP kinaseΒ-spectrinRegeneration genesGene screenMolecular eventsGenesElegansRobust regenerationMature neuronsMutantsMammalian CNSAntagonistic activityPathway
2013
Neuron-Specific Feeding RNAi in C. elegans and Its Use in a Screen for Essential Genes Required for GABA Neuron Function
Firnhaber C, Hammarlund M. Neuron-Specific Feeding RNAi in C. elegans and Its Use in a Screen for Essential Genes Required for GABA Neuron Function. PLOS Genetics 2013, 9: e1003921. PMID: 24244189, PMCID: PMC3820814, DOI: 10.1371/journal.pgen.1003921.Peer-Reviewed Original ResearchConceptsCell-specific knockdownEssential genesFeeding RNAiC. elegansGenetic requirementsNeuronal functionForward genetic screenGABA neuron functionGene function resultsForward screenGenetic screenRNAi resultsRNAi strainsChromosome IRNAi clonesGlutamate-releasing neuronsGene actionGene knockdownMature organismRNAiGenesKnockdownNeuron functionElegansPleiotropyRapid and Permanent Neuronal Inactivation In Vivo via Subcellular Generation of Reactive Oxygen with the Use of KillerRed
Williams DC, Bejjani RE, Ramirez PM, Coakley S, Kim SA, Lee H, Wen Q, Samuel A, Lu H, Hilliard MA, Hammarlund M. Rapid and Permanent Neuronal Inactivation In Vivo via Subcellular Generation of Reactive Oxygen with the Use of KillerRed. Cell Reports 2013, 5: 553-563. PMID: 24209746, PMCID: PMC3877846, DOI: 10.1016/j.celrep.2013.09.023.Peer-Reviewed Original ResearchConceptsReactive oxygen speciesC. elegansGenetic toolsOrganelle fragmentationPlasma membraneSpecific developmental outcomesSubcellular responsesCell deathKillerRedReactive oxygenOxygen speciesTargeted cellCircuit functionSingle light stimulusSingle animalInactivationElegansCellsVivoCell bodiesSpeciesNeuronal degenerationNeuronsAnimalsNeuronal inactivation
2012
Neural Regeneration in Caenorhabditis elegans
Bejjani R, Hammarlund M. Neural Regeneration in Caenorhabditis elegans. Annual Review Of Genetics 2012, 46: 499-513. PMID: 22974301, PMCID: PMC3700416, DOI: 10.1146/annurev-genet-110711-155550.Peer-Reviewed Original Research