2016
Complexin 3 Increases the Fidelity of Signaling in a Retinal Circuit by Regulating Exocytosis at Ribbon Synapses
Mortensen LS, Park SJ, Ke JB, Cooper BH, Zhang L, Imig C, Löwel S, Reim K, Brose N, Demb JB, Rhee JS, Singer JH. Complexin 3 Increases the Fidelity of Signaling in a Retinal Circuit by Regulating Exocytosis at Ribbon Synapses. Cell Reports 2016, 15: 2239-2250. PMID: 27239031, PMCID: PMC5134263, DOI: 10.1016/j.celrep.2016.05.012.Peer-Reviewed Original ResearchConceptsRod bipolarsAsynchronous releaseAmacrine cell synapsesRetinal ganglion cellsRetinal pathwaysGanglion cellsCell synapsesRetinal circuitsRibbon synapsesMouse retinaMultivesicular releaseNeural circuitsComplexin proteinsSynapsesCircuit functionCplx3SignalingStudy linksReleaseExocytosisRB outputRetina
2014
Kainate Receptors Mediate Signaling in Both Transient and Sustained OFF Bipolar Cell Pathways in Mouse Retina
Borghuis BG, Looger LL, Tomita S, Demb JB. Kainate Receptors Mediate Signaling in Both Transient and Sustained OFF Bipolar Cell Pathways in Mouse Retina. Journal Of Neuroscience 2014, 34: 6128-6139. PMID: 24790183, PMCID: PMC4004803, DOI: 10.1523/jneurosci.4941-13.2014.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsDrug InteractionsExcitatory Amino Acid AntagonistsFemaleGlutamic AcidHexamethoniumIn Vitro TechniquesLightMaleMiceMice, Inbred C57BLNicotinic AntagonistsPatch-Clamp TechniquesPhotic StimulationPropionatesReceptors, Kainic AcidRetinaRetinal Bipolar CellsSignal TransductionVisual PathwaysConceptsGlutamate releaseBipolar cellsCone bipolar cell typesOFF bipolar cell pathwaysMouse bipolar cellsGanglion cell dendritesKainate-type glutamate receptorsBipolar cell typesLight-evoked responsesBipolar cell pathwaysGanglion cellsOFF layerCell dendritesReceptor mediatesGlutamate receptorsKainate receptorsAMPA receptorsTwo-photon imagingMouse retinaBipolar pathwayIntact circuitsOFF pathwaysElectrophysiological recordingsCircuit mechanismsReceptorsExcitatory Synaptic Inputs to Mouse On-Off Direction-Selective Retinal Ganglion Cells Lack Direction Tuning
Park SJ, Kim IJ, Looger LL, Demb JB, Borghuis BG. Excitatory Synaptic Inputs to Mouse On-Off Direction-Selective Retinal Ganglion Cells Lack Direction Tuning. Journal Of Neuroscience 2014, 34: 3976-3981. PMID: 24623775, PMCID: PMC3951696, DOI: 10.1523/jneurosci.5017-13.2014.Peer-Reviewed Original ResearchConceptsDirection-selective ganglion cellsOFF direction-selective ganglion cellsStarburst amacrine cellsGlutamate releaseBipolar cellsExcitatory currentsPreferred direction motionBipolar cell typesExcitatory synaptic inputsNull-direction inhibitionGABA-A receptorsWhole-cell recordingsNull-direction motionDSGC dendritesGABA releaseCholinergic inputAmacrine cellsGanglion cellsSynaptic mechanismsSynaptic inputsMammalian retinaMouse retinaIntensity-based glutamate-sensing fluorescent reporterDirection selectivityDirection tuningDevelopmental Changes in NMDA Receptor Subunit Composition at ON and OFF Bipolar Cell Synapses onto Direction-Selective Retinal Ganglion Cells
Stafford BK, Park SJ, Wong KY, Demb JB. Developmental Changes in NMDA Receptor Subunit Composition at ON and OFF Bipolar Cell Synapses onto Direction-Selective Retinal Ganglion Cells. Journal Of Neuroscience 2014, 34: 1942-1948. PMID: 24478373, PMCID: PMC3905152, DOI: 10.1523/jneurosci.4461-13.2014.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnimalsAnimals, NewbornExcitatory Amino Acid AgonistsFemaleGene Expression Regulation, DevelopmentalGlutamic AcidGreen Fluorescent ProteinsIn Vitro TechniquesLightMaleMembrane PotentialsMiceMice, Inbred C57BLMice, TransgenicNeurotransmitter AgentsN-MethylaspartatePatch-Clamp TechniquesReceptors, N-Methyl-D-AspartateRetinaRetinal Bipolar CellsRetinal Ganglion CellsSynapsesVisual PathwaysConceptsNMDA-type glutamate receptorsDirection-selective ganglion cellsExtrasynaptic NMDA-type glutamate receptorsNMDAR-mediated responsesBipolar cell synapsesGanglion cellsCell synapsesMature retinaSpecific ganglion cell typesDirection-selective retinal ganglion cellsNMDA receptor subunit compositionActivity-dependent synaptic developmentBipolar cell inputsGanglion cell typesRetinal ganglion cellsFirst postnatal monthReceptor subunit compositionWhole-cell recordingsGlutamatergic synapse formationCell typesGluN2B antagonist ifenprodilReceptor blockadeGlutamate releaseBipolar synapsesGlutamatergic synapses
2013
Adaptation to Background Light Enables Contrast Coding at Rod Bipolar Cell Synapses
Ke JB, Wang YV, Borghuis BG, Cembrowski MS, Riecke H, Kath WL, Demb JB, Singer JH. Adaptation to Background Light Enables Contrast Coding at Rod Bipolar Cell Synapses. Neuron 2013, 81: 388-401. PMID: 24373883, PMCID: PMC4267681, DOI: 10.1016/j.neuron.2013.10.054.Peer-Reviewed Original ResearchMeSH Keywords2-Amino-5-phosphonovalerateAdaptation, OcularAnimalsBiophysicsComputer SimulationExcitatory Amino Acid AntagonistsGlucosamine 6-Phosphate N-AcetyltransferaseGreen Fluorescent ProteinsIn Vitro TechniquesLightMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicModels, NeurologicalPatch-Clamp TechniquesQuinoxalinesRetinal Bipolar CellsRetinal Rod Photoreceptor CellsSynapsesVisual Pathways
2012
Intrinsic properties and functional circuitry of the AII amacrine cell
DEMB JB, SINGER JH. Intrinsic properties and functional circuitry of the AII amacrine cell. Visual Neuroscience 2012, 29: 51-60. PMID: 22310372, PMCID: PMC3561778, DOI: 10.1017/s0952523811000368.Peer-Reviewed Original ResearchConceptsAII amacrine cellsAmacrine cellsPhotopic conditionsOFF ganglion cellsRod amacrine cellsCone bipolar cellsGanglion cell typesCone-mediated visionRod-mediated visionAII networkCell typesGanglion cellsRetinal neuronsExcitatory interneuronsBipolar cellsMammalian retinaFunctional circuitryAIIDistinct cell typesOutput neuronsNeuronsInhibition pathwayMotion sensitivityPhotoreceptor pathwaysCells
2011
A Synaptic Mechanism for Retinal Adaptation to Luminance and Contrast
Jarsky T, Cembrowski M, Logan SM, Kath WL, Riecke H, Demb JB, Singer JH. A Synaptic Mechanism for Retinal Adaptation to Luminance and Contrast. Journal Of Neuroscience 2011, 31: 11003-11015. PMID: 21795549, PMCID: PMC3152984, DOI: 10.1523/jneurosci.2631-11.2011.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalAmacrine CellsAnimalsBiophysical PhenomenaBiophysicsCalciumContrast SensitivityElectric StimulationExcitatory Postsynaptic PotentialsFemaleIn Vitro TechniquesLightingMaleMiceMice, Inbred C57BLModels, NeurologicalNumerical Analysis, Computer-AssistedPatch-Clamp TechniquesPhotic StimulationPresynaptic TerminalsRetinaRetinal Bipolar CellsSynaptic TransmissionConceptsSynaptic mechanismsSynaptic transmissionBipolar cell synaptic transmissionMouse retinal slice preparationVesicle depletionCommon synaptic mechanismsPrimary sensory circuitsUse-dependent regulationAII amacrine cellsContrast adaptationRetinal slice preparationBipolar cell synapsesCell synaptic transmissionRetinal neural circuitWhole-cell recordingsRetinal bipolar cellsPhasic transmissionTonic transmissionAmacrine cellsSlice preparationCell synapsesBipolar cellsSame cellular mechanismsSensory circuitsNeural circuits
2009
Activity acts locally
Demb JB, Feller MB. Activity acts locally. Nature 2009, 460: 961-963. PMID: 19693075, DOI: 10.1038/460961a.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAxonsMiceRetinaRetinal Bipolar CellsRetinal Ganglion CellsSynapsesSynaptic Transmission
2007
Cellular Basis for Contrast Gain Control over the Receptive Field Center of Mammalian Retinal Ganglion Cells
Beaudoin DL, Borghuis BG, Demb JB. Cellular Basis for Contrast Gain Control over the Receptive Field Center of Mammalian Retinal Ganglion Cells. Journal Of Neuroscience 2007, 27: 2636-2645. PMID: 17344401, PMCID: PMC6672510, DOI: 10.1523/jneurosci.4610-06.2007.Peer-Reviewed Original ResearchConceptsReceptive field centerPresynaptic bipolar cellsGanglion cellsBipolar cellsAmacrine cellsGanglion cell receptive field centersContrast gain controlMammalian retinal ganglion cellsMammalian ganglion cellsRetinal ganglion cellsWhole-cell recordingsInhibitory neurotransmitter receptorsField centerVoltage-clamp conditionsInhibitory interneuronsSynaptic releaseNeurotransmitter receptorsCenter responseExtracellular recordingsPharmacological conditionsAdequate stimulationInhibitory conductanceCalcium bufferingSubthreshold responsesMembrane currents
2006
Making selective 'cone-ections'
Demb JB. Making selective 'cone-ections'. Nature Neuroscience 2006, 9: 595-596. PMID: 16639404, DOI: 10.1038/nn0506-595.Commentaries, Editorials and LettersPresynaptic Mechanism for Slow Contrast Adaptation in Mammalian Retinal Ganglion Cells
Manookin MB, Demb JB. Presynaptic Mechanism for Slow Contrast Adaptation in Mammalian Retinal Ganglion Cells. Neuron 2006, 50: 453-464. PMID: 16675399, DOI: 10.1016/j.neuron.2006.03.039.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAdaptation, PhysiologicalAnimalsCalcium ChannelsContrast SensitivityGlutamic AcidGuinea PigsOrgan Culture TechniquesPhotic StimulationPresynaptic TerminalsReaction TimeReceptors, GABAReceptors, GlycineReceptors, Metabotropic GlutamateRetinal Bipolar CellsRetinal Ganglion CellsSynaptic TransmissionConceptsPresynaptic bipolar cellsRetinal ganglion cellsGanglion cellsBipolar cellsMammalian retinal ganglion cellsSlow contrast adaptationReduced glutamate releaseIntrinsic cellular propertiesIntrinsic cellular mechanismsCortical adaptationGlutamate releasePresynaptic mechanismsGlycine receptorsVisual neuronsAfterhyperpolarizationExcitatory conductancePotassium conductanceContrast adaptationCellular mechanismsCortical cellsNeuronsCellsCellular propertiesMGluRsSpatial stimuli