2015
Function and Circuitry of VIP+ Interneurons in the Mouse Retina
Park SJ, Borghuis BG, Rahmani P, Zeng Q, Kim IJ, Demb JB. Function and Circuitry of VIP+ Interneurons in the Mouse Retina. Journal Of Neuroscience 2015, 35: 10685-10700. PMID: 26224854, PMCID: PMC4518048, DOI: 10.1523/jneurosci.0222-15.2015.Peer-Reviewed Original ResearchConceptsGanglion cell layerAmacrine cell typesInner nuclear layerAmacrine cellsGanglion cellsINL cellsON pathwayGanglion cell dendritesGanglion cell typesBipolar cell terminalsWhole-cell recordingsCell typesNervous system functionSpatial tuningBipolar cell pathwaysTransgenic mouse lineGCL cellsOptic nerveGABAergic interneuronsRetinal functionCell dendritesRetinal circuitryInhibitory interneuronsNuclear layerBipolar interneurons
2014
Kainate Receptors Mediate Signaling in Both Transient and Sustained OFF Bipolar Cell Pathways in Mouse Retina
Borghuis BG, Looger LL, Tomita S, Demb JB. Kainate Receptors Mediate Signaling in Both Transient and Sustained OFF Bipolar Cell Pathways in Mouse Retina. Journal Of Neuroscience 2014, 34: 6128-6139. PMID: 24790183, PMCID: PMC4004803, DOI: 10.1523/jneurosci.4941-13.2014.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsDrug InteractionsExcitatory Amino Acid AntagonistsFemaleGlutamic AcidHexamethoniumIn Vitro TechniquesLightMaleMiceMice, Inbred C57BLNicotinic AntagonistsPatch-Clamp TechniquesPhotic StimulationPropionatesReceptors, Kainic AcidRetinaRetinal Bipolar CellsSignal TransductionVisual PathwaysConceptsGlutamate releaseBipolar cellsCone bipolar cell typesOFF bipolar cell pathwaysMouse bipolar cellsGanglion cell dendritesKainate-type glutamate receptorsBipolar cell typesLight-evoked responsesBipolar cell pathwaysGanglion cellsOFF layerCell dendritesReceptor mediatesGlutamate receptorsKainate receptorsAMPA receptorsTwo-photon imagingMouse retinaBipolar pathwayIntact circuitsOFF pathwaysElectrophysiological recordingsCircuit mechanismsReceptorsDevelopmental Changes in NMDA Receptor Subunit Composition at ON and OFF Bipolar Cell Synapses onto Direction-Selective Retinal Ganglion Cells
Stafford BK, Park SJ, Wong KY, Demb JB. Developmental Changes in NMDA Receptor Subunit Composition at ON and OFF Bipolar Cell Synapses onto Direction-Selective Retinal Ganglion Cells. Journal Of Neuroscience 2014, 34: 1942-1948. PMID: 24478373, PMCID: PMC3905152, DOI: 10.1523/jneurosci.4461-13.2014.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnimalsAnimals, NewbornExcitatory Amino Acid AgonistsFemaleGene Expression Regulation, DevelopmentalGlutamic AcidGreen Fluorescent ProteinsIn Vitro TechniquesLightMaleMembrane PotentialsMiceMice, Inbred C57BLMice, TransgenicNeurotransmitter AgentsN-MethylaspartatePatch-Clamp TechniquesReceptors, N-Methyl-D-AspartateRetinaRetinal Bipolar CellsRetinal Ganglion CellsSynapsesVisual PathwaysConceptsNMDA-type glutamate receptorsDirection-selective ganglion cellsExtrasynaptic NMDA-type glutamate receptorsNMDAR-mediated responsesBipolar cell synapsesGanglion cellsCell synapsesMature retinaSpecific ganglion cell typesDirection-selective retinal ganglion cellsNMDA receptor subunit compositionActivity-dependent synaptic developmentBipolar cell inputsGanglion cell typesRetinal ganglion cellsFirst postnatal monthReceptor subunit compositionWhole-cell recordingsGlutamatergic synapse formationCell typesGluN2B antagonist ifenprodilReceptor blockadeGlutamate releaseBipolar synapsesGlutamatergic synapses
2013
Adaptation to Background Light Enables Contrast Coding at Rod Bipolar Cell Synapses
Ke JB, Wang YV, Borghuis BG, Cembrowski MS, Riecke H, Kath WL, Demb JB, Singer JH. Adaptation to Background Light Enables Contrast Coding at Rod Bipolar Cell Synapses. Neuron 2013, 81: 388-401. PMID: 24373883, PMCID: PMC4267681, DOI: 10.1016/j.neuron.2013.10.054.Peer-Reviewed Original ResearchMeSH Keywords2-Amino-5-phosphonovalerateAdaptation, OcularAnimalsBiophysicsComputer SimulationExcitatory Amino Acid AntagonistsGlucosamine 6-Phosphate N-AcetyltransferaseGreen Fluorescent ProteinsIn Vitro TechniquesLightMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicModels, NeurologicalPatch-Clamp TechniquesQuinoxalinesRetinal Bipolar CellsRetinal Rod Photoreceptor CellsSynapsesVisual Pathways
2012
Form and Function of the M4 Cell, an Intrinsically Photosensitive Retinal Ganglion Cell Type Contributing to Geniculocortical Vision
Estevez ME, Fogerson PM, Ilardi MC, Borghuis BG, Chan E, Weng S, Auferkorte ON, Demb JB, Berson DM. Form and Function of the M4 Cell, an Intrinsically Photosensitive Retinal Ganglion Cell Type Contributing to Geniculocortical Vision. Journal Of Neuroscience 2012, 32: 13608-13620. PMID: 23015450, PMCID: PMC3474539, DOI: 10.1523/jneurosci.1422-12.2012.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBeta-GalactosidaseCholera ToxinCholine O-AcetyltransferaseDendritesElectroretinographyFemaleGeniculate BodiesGreen Fluorescent ProteinsLightMaleMembrane PotentialsMiceMice, Inbred C57BLMice, TransgenicPatch-Clamp TechniquesPhotic StimulationRetinaRetinal Ganglion CellsRod OpsinsVisual CortexVisual FieldsVisual PathwaysConceptsM4 cellsM2 cellsDorsal lateral geniculate nucleusPhotosensitive retinal ganglion cellsInner plexiform layerRetinal ganglion cellsLateral geniculate nucleusRetinal output neuronsNonlinear spatial summationCholinergic bandDendritic stratificationON sublaminaLarge somataPlexiform layerGanglion cellsDendritic arborsGeniculate nucleusPhotopigment melanopsinVentral retinaDorsal retinaMouse retinaDirection selectivityFunctional rodsCentral projectionsM3 cells
2011
A Synaptic Mechanism for Retinal Adaptation to Luminance and Contrast
Jarsky T, Cembrowski M, Logan SM, Kath WL, Riecke H, Demb JB, Singer JH. A Synaptic Mechanism for Retinal Adaptation to Luminance and Contrast. Journal Of Neuroscience 2011, 31: 11003-11015. PMID: 21795549, PMCID: PMC3152984, DOI: 10.1523/jneurosci.2631-11.2011.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalAmacrine CellsAnimalsBiophysical PhenomenaBiophysicsCalciumContrast SensitivityElectric StimulationExcitatory Postsynaptic PotentialsFemaleIn Vitro TechniquesLightingMaleMiceMice, Inbred C57BLModels, NeurologicalNumerical Analysis, Computer-AssistedPatch-Clamp TechniquesPhotic StimulationPresynaptic TerminalsRetinaRetinal Bipolar CellsSynaptic TransmissionConceptsSynaptic mechanismsSynaptic transmissionBipolar cell synaptic transmissionMouse retinal slice preparationVesicle depletionCommon synaptic mechanismsPrimary sensory circuitsUse-dependent regulationAII amacrine cellsContrast adaptationRetinal slice preparationBipolar cell synapsesCell synaptic transmissionRetinal neural circuitWhole-cell recordingsRetinal bipolar cellsPhasic transmissionTonic transmissionAmacrine cellsSlice preparationCell synapsesBipolar cellsSame cellular mechanismsSensory circuitsNeural circuitsDelayed-Rectifier K Channels Contribute to Contrast Adaptation in Mammalian Retinal Ganglion Cells
Weick M, Demb JB. Delayed-Rectifier K Channels Contribute to Contrast Adaptation in Mammalian Retinal Ganglion Cells. Neuron 2011, 71: 166-179. PMID: 21745646, PMCID: PMC3134798, DOI: 10.1016/j.neuron.2011.04.033.Peer-Reviewed Original ResearchConceptsRetinal ganglion cellsGanglion cellsSubsequent depolarizationContrast adaptationOFF alpha ganglion cellsMammalian retinal ganglion cellsAlpha ganglion cellsGuinea pig retinaK channelsSuppress excitabilityPig retinaNa channel inactivationFiring responsesBrief depolarizationBrief hyperpolarizationRectifier K channelsVisual stimulationSomatic membrane patchesChannel inactivationDepolarizationPhysiological rangeIntrinsic mechanismHyperpolarizationCellsSalamander cells
2010
NMDA Receptor Contributions to Visual Contrast Coding
Manookin MB, Weick M, Stafford BK, Demb JB. NMDA Receptor Contributions to Visual Contrast Coding. Neuron 2010, 67: 280-293. PMID: 20670835, PMCID: PMC2913150, DOI: 10.1016/j.neuron.2010.06.020.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsContrast SensitivityDizocilpine MaleateElectric ConductivityExcitatory Amino Acid AgonistsExcitatory Amino Acid AntagonistsGene Expression RegulationGuinea PigsIn Vitro TechniquesLightMiceMice, Inbred C57BLN-MethylaspartatePatch-Clamp TechniquesPhotic StimulationPiperidinesReceptors, N-Methyl-D-AspartateRetinaRetinal Ganglion CellsVisual PerceptionConceptsNMDA receptorsOFF alphaAlpha cellsNMDA-type glutamate receptorsOFF alpha cellsDelta cellsGanglion cell typesNMDA receptor contributionCell typesExcitatory responsesGlutamate receptorsInhibitory currentsAMPA receptorsReceptor contributionGluN2B subunitContrast sensitivityContrast codingReceptorsNMDAAMPACertain cell typesCellsVisual processingAlpha
2007
Functional Circuitry for Peripheral Suppression in Mammalian Y-Type Retinal Ganglion Cells
Zaghloul KA, Manookin MB, Borghuis BG, Boahen K, Demb JB. Functional Circuitry for Peripheral Suppression in Mammalian Y-Type Retinal Ganglion Cells. Journal Of Neurophysiology 2007, 97: 4327-4340. PMID: 17460102, DOI: 10.1152/jn.01091.2006.Peer-Reviewed Original ResearchConceptsAmacrine cellsGanglion cellsCenter responseSynaptic layersY-type retinal ganglion cellsHorizontal cellsY-type ganglion cellsSecond synaptic layerGanglion cell receptive fieldsRetinal ganglion cellsGuinea pig retinaStimulus-response curvesRetinal ganglion cell receptive fieldsFirst synaptic layerCurrent-clamp conditionsBipolar terminalsGlutamate releaseCell receptive fieldsPeripheral gratingsPeripheral suppressionIntracellular recordingsPig retinaSubthreshold membrane potentialsExcitatory centerInhibitory conductance