2014
Kainate Receptors Mediate Signaling in Both Transient and Sustained OFF Bipolar Cell Pathways in Mouse Retina
Borghuis BG, Looger LL, Tomita S, Demb JB. Kainate Receptors Mediate Signaling in Both Transient and Sustained OFF Bipolar Cell Pathways in Mouse Retina. Journal Of Neuroscience 2014, 34: 6128-6139. PMID: 24790183, PMCID: PMC4004803, DOI: 10.1523/jneurosci.4941-13.2014.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsDrug InteractionsExcitatory Amino Acid AntagonistsFemaleGlutamic AcidHexamethoniumIn Vitro TechniquesLightMaleMiceMice, Inbred C57BLNicotinic AntagonistsPatch-Clamp TechniquesPhotic StimulationPropionatesReceptors, Kainic AcidRetinaRetinal Bipolar CellsSignal TransductionVisual PathwaysConceptsGlutamate releaseBipolar cellsCone bipolar cell typesOFF bipolar cell pathwaysMouse bipolar cellsGanglion cell dendritesKainate-type glutamate receptorsBipolar cell typesLight-evoked responsesBipolar cell pathwaysGanglion cellsOFF layerCell dendritesReceptor mediatesGlutamate receptorsKainate receptorsAMPA receptorsTwo-photon imagingMouse retinaBipolar pathwayIntact circuitsOFF pathwaysElectrophysiological recordingsCircuit mechanismsReceptors
2013
Two-Photon Imaging of Nonlinear Glutamate Release Dynamics at Bipolar Cell Synapses in the Mouse Retina
Borghuis BG, Marvin JS, Looger LL, Demb JB. Two-Photon Imaging of Nonlinear Glutamate Release Dynamics at Bipolar Cell Synapses in the Mouse Retina. Journal Of Neuroscience 2013, 33: 10972-10985. PMID: 23825403, PMCID: PMC3718381, DOI: 10.1523/jneurosci.1241-13.2013.Peer-Reviewed Original ResearchConceptsInner plexiform layerGanglion cellsPlexiform layerY-type retinal ganglion cellsY-type ganglion cellsPresynaptic bipolar cellsBipolar cell outputBipolar cell synapsesRetinal ganglion cellsWhole-cell recordingsPostsynaptic ganglion cellsIntact neural circuitsReceptive field propertiesPresynaptic release sitesBipolar terminalsGlutamate releaseGlutamate synapsesSynaptic basisCell synapsesBipolar cellsExcitatory currentsSurround inhibitionMouse retinaTwo-photon imagingIndependent releaseAn optimized fluorescent probe for visualizing glutamate neurotransmission
Marvin JS, Borghuis BG, Tian L, Cichon J, Harnett MT, Akerboom J, Gordus A, Renninger SL, Chen TW, Bargmann CI, Orger MB, Schreiter ER, Demb JB, Gan WB, Hires SA, Looger LL. An optimized fluorescent probe for visualizing glutamate neurotransmission. Nature Methods 2013, 10: 162-170. PMID: 23314171, PMCID: PMC4469972, DOI: 10.1038/nmeth.2333.Peer-Reviewed Original ResearchAnimalsAstrocytesBiosensing TechniquesCaenorhabditis elegansCalcium SignalingEscherichia coli ProteinsExcitatory Postsynaptic PotentialsFluorescent DyesGlutamic AcidGreen Fluorescent ProteinsHippocampusMiceMotor CortexNeuronsPhotic StimulationPyramidal CellsRecombinant Fusion ProteinsRetinaSignal-To-Noise RatioSynaptic TransmissionZebrafish
2012
Form and Function of the M4 Cell, an Intrinsically Photosensitive Retinal Ganglion Cell Type Contributing to Geniculocortical Vision
Estevez ME, Fogerson PM, Ilardi MC, Borghuis BG, Chan E, Weng S, Auferkorte ON, Demb JB, Berson DM. Form and Function of the M4 Cell, an Intrinsically Photosensitive Retinal Ganglion Cell Type Contributing to Geniculocortical Vision. Journal Of Neuroscience 2012, 32: 13608-13620. PMID: 23015450, PMCID: PMC3474539, DOI: 10.1523/jneurosci.1422-12.2012.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBeta-GalactosidaseCholera ToxinCholine O-AcetyltransferaseDendritesElectroretinographyFemaleGeniculate BodiesGreen Fluorescent ProteinsLightMaleMembrane PotentialsMiceMice, Inbred C57BLMice, TransgenicPatch-Clamp TechniquesPhotic StimulationRetinaRetinal Ganglion CellsRod OpsinsVisual CortexVisual FieldsVisual PathwaysConceptsM4 cellsM2 cellsDorsal lateral geniculate nucleusPhotosensitive retinal ganglion cellsInner plexiform layerRetinal ganglion cellsLateral geniculate nucleusRetinal output neuronsNonlinear spatial summationCholinergic bandDendritic stratificationON sublaminaLarge somataPlexiform layerGanglion cellsDendritic arborsGeniculate nucleusPhotopigment melanopsinVentral retinaDorsal retinaMouse retinaDirection selectivityFunctional rodsCentral projectionsM3 cells
2011
A Synaptic Mechanism for Retinal Adaptation to Luminance and Contrast
Jarsky T, Cembrowski M, Logan SM, Kath WL, Riecke H, Demb JB, Singer JH. A Synaptic Mechanism for Retinal Adaptation to Luminance and Contrast. Journal Of Neuroscience 2011, 31: 11003-11015. PMID: 21795549, PMCID: PMC3152984, DOI: 10.1523/jneurosci.2631-11.2011.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalAmacrine CellsAnimalsBiophysical PhenomenaBiophysicsCalciumContrast SensitivityElectric StimulationExcitatory Postsynaptic PotentialsFemaleIn Vitro TechniquesLightingMaleMiceMice, Inbred C57BLModels, NeurologicalNumerical Analysis, Computer-AssistedPatch-Clamp TechniquesPhotic StimulationPresynaptic TerminalsRetinaRetinal Bipolar CellsSynaptic TransmissionConceptsSynaptic mechanismsSynaptic transmissionBipolar cell synaptic transmissionMouse retinal slice preparationVesicle depletionCommon synaptic mechanismsPrimary sensory circuitsUse-dependent regulationAII amacrine cellsContrast adaptationRetinal slice preparationBipolar cell synapsesCell synaptic transmissionRetinal neural circuitWhole-cell recordingsRetinal bipolar cellsPhasic transmissionTonic transmissionAmacrine cellsSlice preparationCell synapsesBipolar cellsSame cellular mechanismsSensory circuitsNeural circuitsDelayed-Rectifier K Channels Contribute to Contrast Adaptation in Mammalian Retinal Ganglion Cells
Weick M, Demb JB. Delayed-Rectifier K Channels Contribute to Contrast Adaptation in Mammalian Retinal Ganglion Cells. Neuron 2011, 71: 166-179. PMID: 21745646, PMCID: PMC3134798, DOI: 10.1016/j.neuron.2011.04.033.Peer-Reviewed Original ResearchConceptsRetinal ganglion cellsGanglion cellsSubsequent depolarizationContrast adaptationOFF alpha ganglion cellsMammalian retinal ganglion cellsAlpha ganglion cellsGuinea pig retinaK channelsSuppress excitabilityPig retinaNa channel inactivationFiring responsesBrief depolarizationBrief hyperpolarizationRectifier K channelsVisual stimulationSomatic membrane patchesChannel inactivationDepolarizationPhysiological rangeIntrinsic mechanismHyperpolarizationCellsSalamander cellsSpectral and Temporal Sensitivity of Cone-Mediated Responses in Mouse Retinal Ganglion Cells
Wang YV, Weick M, Demb JB. Spectral and Temporal Sensitivity of Cone-Mediated Responses in Mouse Retinal Ganglion Cells. Journal Of Neuroscience 2011, 31: 7670-7681. PMID: 21613480, PMCID: PMC3122925, DOI: 10.1523/jneurosci.0629-11.2011.Peer-Reviewed Original ResearchConceptsCone-mediated responsesM-opsin expressionGanglion cellsMouse retinaMouse retinal ganglion cellsRod-mediated responsesSlower temporal processingRetinal ganglion cellsWild-type miceGanglion cell responsesCone-mediated visionMouse ganglion cellsVentral retinaDorsal retinaRod functionCone functionCell responsesS-opsinRetinaMiceMouse photoreceptorsExpression ratioLight stimulationPhotoreceptor typesTemporal processing
2010
NMDA Receptor Contributions to Visual Contrast Coding
Manookin MB, Weick M, Stafford BK, Demb JB. NMDA Receptor Contributions to Visual Contrast Coding. Neuron 2010, 67: 280-293. PMID: 20670835, PMCID: PMC2913150, DOI: 10.1016/j.neuron.2010.06.020.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsContrast SensitivityDizocilpine MaleateElectric ConductivityExcitatory Amino Acid AgonistsExcitatory Amino Acid AntagonistsGene Expression RegulationGuinea PigsIn Vitro TechniquesLightMiceMice, Inbred C57BLN-MethylaspartatePatch-Clamp TechniquesPhotic StimulationPiperidinesReceptors, N-Methyl-D-AspartateRetinaRetinal Ganglion CellsVisual PerceptionConceptsNMDA receptorsOFF alphaAlpha cellsNMDA-type glutamate receptorsOFF alpha cellsDelta cellsGanglion cell typesNMDA receptor contributionCell typesExcitatory responsesGlutamate receptorsInhibitory currentsAMPA receptorsReceptor contributionGluN2B subunitContrast sensitivityContrast codingReceptorsNMDAAMPACertain cell typesCellsVisual processingAlpha
2008
Distinct expressions of contrast gain control in parallel synaptic pathways converging on a retinal ganglion cell
Beaudoin DL, Manookin MB, Demb JB. Distinct expressions of contrast gain control in parallel synaptic pathways converging on a retinal ganglion cell. The Journal Of Physiology 2008, 586: 5487-5502. PMID: 18832424, PMCID: PMC2655385, DOI: 10.1113/jphysiol.2008.156224.Peer-Reviewed Original ResearchConceptsCone bipolar cellsOFF ganglion cellsRod bipolar cellsGanglion cellsRetinal ganglion cellsBipolar cellsInhibitory inputsExcitatory inputsSynaptic pathwaysContrast gain controlOFF cone bipolar cellsIonotropic glutamate receptor antagonistsY-type ganglion cellsRod bipolar pathwayAII amacrine cellsGlutamate receptor antagonistsGanglion cell firingWhole-cell recordingsLoose-patch recordingsAmacrine cellsReceptor antagonistRetinal pathwaysAII cellsSynaptic mechanismsSynaptic currentsDisinhibition Combines with Excitation to Extend the Operating Range of the OFF Visual Pathway in Daylight
Manookin MB, Beaudoin DL, Ernst ZR, Flagel LJ, Demb JB. Disinhibition Combines with Excitation to Extend the Operating Range of the OFF Visual Pathway in Daylight. Journal Of Neuroscience 2008, 28: 4136-4150. PMID: 18417693, PMCID: PMC2557439, DOI: 10.1523/jneurosci.4274-07.2008.Peer-Reviewed Original ResearchConceptsOFF ganglion cellsAII amacrine cellsCone bipolar cellsGanglion cellsAmacrine cellsAII cellsBipolar cellsPresence of CNQXBipolar cell synapsesD-APOFF visual pathwayBipolar cell pathwaysGap junctionsGlycine releaseExcitatory responsesIonotropic glutamateSynaptic inhibitionCell synapsesON pathwayGlycine receptorsLight decrementsVisual pathwayElectrical synapsesMeclofenamic acidDaylight vision
2007
Functional Circuitry for Peripheral Suppression in Mammalian Y-Type Retinal Ganglion Cells
Zaghloul KA, Manookin MB, Borghuis BG, Boahen K, Demb JB. Functional Circuitry for Peripheral Suppression in Mammalian Y-Type Retinal Ganglion Cells. Journal Of Neurophysiology 2007, 97: 4327-4340. PMID: 17460102, DOI: 10.1152/jn.01091.2006.Peer-Reviewed Original ResearchConceptsAmacrine cellsGanglion cellsCenter responseSynaptic layersY-type retinal ganglion cellsHorizontal cellsY-type ganglion cellsSecond synaptic layerGanglion cell receptive fieldsRetinal ganglion cellsGuinea pig retinaStimulus-response curvesRetinal ganglion cell receptive fieldsFirst synaptic layerCurrent-clamp conditionsBipolar terminalsGlutamate releaseCell receptive fieldsPeripheral gratingsPeripheral suppressionIntracellular recordingsPig retinaSubthreshold membrane potentialsExcitatory centerInhibitory conductanceCellular Basis for Contrast Gain Control over the Receptive Field Center of Mammalian Retinal Ganglion Cells
Beaudoin DL, Borghuis BG, Demb JB. Cellular Basis for Contrast Gain Control over the Receptive Field Center of Mammalian Retinal Ganglion Cells. Journal Of Neuroscience 2007, 27: 2636-2645. PMID: 17344401, PMCID: PMC6672510, DOI: 10.1523/jneurosci.4610-06.2007.Peer-Reviewed Original ResearchConceptsReceptive field centerPresynaptic bipolar cellsGanglion cellsBipolar cellsAmacrine cellsGanglion cell receptive field centersContrast gain controlMammalian retinal ganglion cellsMammalian ganglion cellsRetinal ganglion cellsWhole-cell recordingsInhibitory neurotransmitter receptorsField centerVoltage-clamp conditionsInhibitory interneuronsSynaptic releaseNeurotransmitter receptorsCenter responseExtracellular recordingsPharmacological conditionsAdequate stimulationInhibitory conductanceCalcium bufferingSubthreshold responsesMembrane currents
2006
Presynaptic Mechanism for Slow Contrast Adaptation in Mammalian Retinal Ganglion Cells
Manookin MB, Demb JB. Presynaptic Mechanism for Slow Contrast Adaptation in Mammalian Retinal Ganglion Cells. Neuron 2006, 50: 453-464. PMID: 16675399, DOI: 10.1016/j.neuron.2006.03.039.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAdaptation, PhysiologicalAnimalsCalcium ChannelsContrast SensitivityGlutamic AcidGuinea PigsOrgan Culture TechniquesPhotic StimulationPresynaptic TerminalsReaction TimeReceptors, GABAReceptors, GlycineReceptors, Metabotropic GlutamateRetinal Bipolar CellsRetinal Ganglion CellsSynaptic TransmissionConceptsPresynaptic bipolar cellsRetinal ganglion cellsGanglion cellsBipolar cellsMammalian retinal ganglion cellsSlow contrast adaptationReduced glutamate releaseIntrinsic cellular propertiesIntrinsic cellular mechanismsCortical adaptationGlutamate releasePresynaptic mechanismsGlycine receptorsVisual neuronsAfterhyperpolarizationExcitatory conductancePotassium conductanceContrast adaptationCellular mechanismsCortical cellsNeuronsCellsCellular propertiesMGluRsSpatial stimuli
2005
Do We Know What the Early Visual System Does?
Carandini M, Demb JB, Mante V, Tolhurst DJ, Dan Y, Olshausen BA, Gallant JL, Rust NC. Do We Know What the Early Visual System Does? Journal Of Neuroscience 2005, 25: 10577-10597. PMID: 16291931, PMCID: PMC6725861, DOI: 10.1523/jneurosci.3726-05.2005.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsContrast Adaptation in Subthreshold and Spiking Responses of Mammalian Y-Type Retinal Ganglion Cells
Zaghloul KA, Boahen K, Demb JB. Contrast Adaptation in Subthreshold and Spiking Responses of Mammalian Y-Type Retinal Ganglion Cells. Journal Of Neuroscience 2005, 25: 860-868. PMID: 15673666, PMCID: PMC6725633, DOI: 10.1523/jneurosci.2782-04.2005.Peer-Reviewed Original ResearchConceptsRetinal ganglion cellsGanglion cellsIntracellular recordingsSubthreshold responsesY-type retinal ganglion cellsY-type ganglion cellsSynaptic mechanismsSynaptic inputsMammalian retinaSpiking responseExtracellular recordingsSubthreshold potentialsContrast sensitivitySpike outputSpike thresholdContrast adaptationSpike generationGain changesCellsRecordingsResponseApparent reductionRecording conditionsAbove effectsMean light level
2004
How Retinal Ganglion Cells Prevent Synaptic Noise From Reaching the Spike Output
Demb JB, Sterling P, Freed MA. How Retinal Ganglion Cells Prevent Synaptic Noise From Reaching the Spike Output. Journal Of Neurophysiology 2004, 92: 2510-2519. PMID: 15175375, DOI: 10.1152/jn.00108.2004.Peer-Reviewed Original ResearchConceptsSynaptic convergenceSpike outputGanglion cellsIntracellular recordingsSynaptic inputsSpike responsesF1 responseRetinal ganglion cellsSpike rateNeuron synaptic inputsPrimary visual cortexExtracellular recordingsVisual cortexPeak depolarizationStimulus frequencyStimulus cycleSynaptic vesiclesStimulus contrastStrong stimulusSynaptic noiseDepolarizationNeuronsParadoxical findingsMembrane potentialSame cells
2003
Different Circuits for ON and OFF Retinal Ganglion Cells Cause Different Contrast Sensitivities
Zaghloul KA, Boahen K, Demb JB. Different Circuits for ON and OFF Retinal Ganglion Cells Cause Different Contrast Sensitivities. Journal Of Neuroscience 2003, 23: 2645-2654. PMID: 12684450, PMCID: PMC6742092, DOI: 10.1523/jneurosci.23-07-02645.2003.Peer-Reviewed Original ResearchConceptsOFF cellsGanglion cellsSynaptic inputsContrast sensitivityExcitatory conductanceOFF retinal ganglion cellsOFF ganglion cellsBasal glutamate releaseRetinal ganglion cellsBrisk-transient cellsGuinea pig retinaBipolar terminalsPresynaptic circuitsGlutamate releaseIntracellular recordingsON pathwayPig retinaSubthreshold membrane potentialsLight incrementsL-APMembrane potentialCellsMean luminancePathwayRetina
2001
Cellular Basis for the Response to Second-Order Motion Cues in Y Retinal Ganglion Cells
Demb J, Zaghloul K, Sterling P. Cellular Basis for the Response to Second-Order Motion Cues in Y Retinal Ganglion Cells. Neuron 2001, 32: 711-721. PMID: 11719210, DOI: 10.1016/s0896-6273(01)00484-6.Peer-Reviewed Original ResearchConceptsGanglion cellsEPSP/IPSP sequencesY cellsOFF ganglion cellsY ganglion cellsRetinal ganglion cellsSecond-order motion cuesSecond-order motion stimuliDendritic fieldsInhibitory effectCell inhibitionCellular basisMotion stimuliCortical cellsSecond-order motionCellsPerceive motionResponseTetrodotoxinCortexRetinaBipolar Cells Contribute to Nonlinear Spatial Summation in the Brisk-Transient (Y) Ganglion Cell in Mammalian Retina
Demb J, Zaghloul K, Haarsma L, Sterling P. Bipolar Cells Contribute to Nonlinear Spatial Summation in the Brisk-Transient (Y) Ganglion Cell in Mammalian Retina. Journal Of Neuroscience 2001, 21: 7447-7454. PMID: 11567034, PMCID: PMC6762908, DOI: 10.1523/jneurosci.21-19-07447.2001.Peer-Reviewed Original ResearchConceptsBipolar cellsGanglion cellsBrisk-transient ganglion cellsReceptive fieldsExcitatory postsynaptic potentialsGuinea pig retinaNonlinear spatial summationNonlinear receptive fieldsAmacrine cellsPostsynaptic potentialsPig retinaExcitatory mechanismsMammalian retinaY cellsLinear receptive fieldsSpatial summationBipolar inputRetinaCellsPeripheral componentsCentral componentAcetylcholineTetrodotoxinGABA
1999
Neuronal basis of contrast discrimination
Boynton G, Demb J, Glover G, Heeger D. Neuronal basis of contrast discrimination. Vision Research 1999, 39: 257-269. PMID: 10326134, DOI: 10.1016/s0042-6989(98)00113-8.Peer-Reviewed Original ResearchMeSH KeywordsContrast SensitivityHumansMagnetic Resonance ImagingPhotic StimulationPsychophysicsVisual CortexConceptsVisual cortical areasAverage neuronal activityFunctional magnetic resonance imagingFMRI responsesNeuronal activityCortical areasEarly visual cortical areasMagnetic resonance imagingVisual areas V1Contrast increment thresholdsIncrement thresholdsEarly visual cortexNeuronal responsesBrain areasVisual cortexResonance imagingArea V1Neuronal basisPooled activityStimulus intervalStimulus contrastBaseline contrastNeuronal signalsBrain activityHuman brain activity