2014
Kainate Receptors Mediate Signaling in Both Transient and Sustained OFF Bipolar Cell Pathways in Mouse Retina
Borghuis BG, Looger LL, Tomita S, Demb JB. Kainate Receptors Mediate Signaling in Both Transient and Sustained OFF Bipolar Cell Pathways in Mouse Retina. Journal Of Neuroscience 2014, 34: 6128-6139. PMID: 24790183, PMCID: PMC4004803, DOI: 10.1523/jneurosci.4941-13.2014.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsDrug InteractionsExcitatory Amino Acid AntagonistsFemaleGlutamic AcidHexamethoniumIn Vitro TechniquesLightMaleMiceMice, Inbred C57BLNicotinic AntagonistsPatch-Clamp TechniquesPhotic StimulationPropionatesReceptors, Kainic AcidRetinaRetinal Bipolar CellsSignal TransductionVisual PathwaysConceptsGlutamate releaseBipolar cellsCone bipolar cell typesOFF bipolar cell pathwaysMouse bipolar cellsGanglion cell dendritesKainate-type glutamate receptorsBipolar cell typesLight-evoked responsesBipolar cell pathwaysGanglion cellsOFF layerCell dendritesReceptor mediatesGlutamate receptorsKainate receptorsAMPA receptorsTwo-photon imagingMouse retinaBipolar pathwayIntact circuitsOFF pathwaysElectrophysiological recordingsCircuit mechanismsReceptorsExcitatory Synaptic Inputs to Mouse On-Off Direction-Selective Retinal Ganglion Cells Lack Direction Tuning
Park SJ, Kim IJ, Looger LL, Demb JB, Borghuis BG. Excitatory Synaptic Inputs to Mouse On-Off Direction-Selective Retinal Ganglion Cells Lack Direction Tuning. Journal Of Neuroscience 2014, 34: 3976-3981. PMID: 24623775, PMCID: PMC3951696, DOI: 10.1523/jneurosci.5017-13.2014.Peer-Reviewed Original ResearchConceptsDirection-selective ganglion cellsOFF direction-selective ganglion cellsStarburst amacrine cellsGlutamate releaseBipolar cellsExcitatory currentsPreferred direction motionBipolar cell typesExcitatory synaptic inputsNull-direction inhibitionGABA-A receptorsWhole-cell recordingsNull-direction motionDSGC dendritesGABA releaseCholinergic inputAmacrine cellsGanglion cellsSynaptic mechanismsSynaptic inputsMammalian retinaMouse retinaIntensity-based glutamate-sensing fluorescent reporterDirection selectivityDirection tuning
2013
Two-Photon Imaging of Nonlinear Glutamate Release Dynamics at Bipolar Cell Synapses in the Mouse Retina
Borghuis BG, Marvin JS, Looger LL, Demb JB. Two-Photon Imaging of Nonlinear Glutamate Release Dynamics at Bipolar Cell Synapses in the Mouse Retina. Journal Of Neuroscience 2013, 33: 10972-10985. PMID: 23825403, PMCID: PMC3718381, DOI: 10.1523/jneurosci.1241-13.2013.Peer-Reviewed Original ResearchConceptsInner plexiform layerGanglion cellsPlexiform layerY-type retinal ganglion cellsY-type ganglion cellsPresynaptic bipolar cellsBipolar cell outputBipolar cell synapsesRetinal ganglion cellsWhole-cell recordingsPostsynaptic ganglion cellsIntact neural circuitsReceptive field propertiesPresynaptic release sitesBipolar terminalsGlutamate releaseGlutamate synapsesSynaptic basisCell synapsesBipolar cellsExcitatory currentsSurround inhibitionMouse retinaTwo-photon imagingIndependent release
2012
Intrinsic properties and functional circuitry of the AII amacrine cell
DEMB JB, SINGER JH. Intrinsic properties and functional circuitry of the AII amacrine cell. Visual Neuroscience 2012, 29: 51-60. PMID: 22310372, PMCID: PMC3561778, DOI: 10.1017/s0952523811000368.Peer-Reviewed Original ResearchConceptsAII amacrine cellsAmacrine cellsPhotopic conditionsOFF ganglion cellsRod amacrine cellsCone bipolar cellsGanglion cell typesCone-mediated visionRod-mediated visionAII networkCell typesGanglion cellsRetinal neuronsExcitatory interneuronsBipolar cellsMammalian retinaFunctional circuitryAIIDistinct cell typesOutput neuronsNeuronsInhibition pathwayMotion sensitivityPhotoreceptor pathwaysCells
2011
A Synaptic Mechanism for Retinal Adaptation to Luminance and Contrast
Jarsky T, Cembrowski M, Logan SM, Kath WL, Riecke H, Demb JB, Singer JH. A Synaptic Mechanism for Retinal Adaptation to Luminance and Contrast. Journal Of Neuroscience 2011, 31: 11003-11015. PMID: 21795549, PMCID: PMC3152984, DOI: 10.1523/jneurosci.2631-11.2011.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalAmacrine CellsAnimalsBiophysical PhenomenaBiophysicsCalciumContrast SensitivityElectric StimulationExcitatory Postsynaptic PotentialsFemaleIn Vitro TechniquesLightingMaleMiceMice, Inbred C57BLModels, NeurologicalNumerical Analysis, Computer-AssistedPatch-Clamp TechniquesPhotic StimulationPresynaptic TerminalsRetinaRetinal Bipolar CellsSynaptic TransmissionConceptsSynaptic mechanismsSynaptic transmissionBipolar cell synaptic transmissionMouse retinal slice preparationVesicle depletionCommon synaptic mechanismsPrimary sensory circuitsUse-dependent regulationAII amacrine cellsContrast adaptationRetinal slice preparationBipolar cell synapsesCell synaptic transmissionRetinal neural circuitWhole-cell recordingsRetinal bipolar cellsPhasic transmissionTonic transmissionAmacrine cellsSlice preparationCell synapsesBipolar cellsSame cellular mechanismsSensory circuitsNeural circuits
2008
Distinct expressions of contrast gain control in parallel synaptic pathways converging on a retinal ganglion cell
Beaudoin DL, Manookin MB, Demb JB. Distinct expressions of contrast gain control in parallel synaptic pathways converging on a retinal ganglion cell. The Journal Of Physiology 2008, 586: 5487-5502. PMID: 18832424, PMCID: PMC2655385, DOI: 10.1113/jphysiol.2008.156224.Peer-Reviewed Original ResearchConceptsCone bipolar cellsOFF ganglion cellsRod bipolar cellsGanglion cellsRetinal ganglion cellsBipolar cellsInhibitory inputsExcitatory inputsSynaptic pathwaysContrast gain controlOFF cone bipolar cellsIonotropic glutamate receptor antagonistsY-type ganglion cellsRod bipolar pathwayAII amacrine cellsGlutamate receptor antagonistsGanglion cell firingWhole-cell recordingsLoose-patch recordingsAmacrine cellsReceptor antagonistRetinal pathwaysAII cellsSynaptic mechanismsSynaptic currentsFunctional circuitry of visual adaptation in the retina
Demb JB. Functional circuitry of visual adaptation in the retina. The Journal Of Physiology 2008, 586: 4377-4384. PMID: 18617564, PMCID: PMC2614018, DOI: 10.1113/jphysiol.2008.156638.Peer-Reviewed Original ResearchConceptsPresynaptic bipolar cellsGanglion cellsReceptive field centerBipolar cellsGanglion cell receptive field centersPeripheral receptive fieldsBipolar cell inputsContrast adaptationRetinal ganglion cellsBipolar terminalsGlutamate releaseAmacrine cellsSynaptic outputMammalian retinaContrast stimulationFunctional circuitryCellular mechanismsSpike generationCell inputHyperpolarizationReceptive fieldsRetinaResponse saturationField centerCellsDisinhibition Combines with Excitation to Extend the Operating Range of the OFF Visual Pathway in Daylight
Manookin MB, Beaudoin DL, Ernst ZR, Flagel LJ, Demb JB. Disinhibition Combines with Excitation to Extend the Operating Range of the OFF Visual Pathway in Daylight. Journal Of Neuroscience 2008, 28: 4136-4150. PMID: 18417693, PMCID: PMC2557439, DOI: 10.1523/jneurosci.4274-07.2008.Peer-Reviewed Original ResearchConceptsOFF ganglion cellsAII amacrine cellsCone bipolar cellsGanglion cellsAmacrine cellsAII cellsBipolar cellsPresence of CNQXBipolar cell synapsesD-APOFF visual pathwayBipolar cell pathwaysGap junctionsGlycine releaseExcitatory responsesIonotropic glutamateSynaptic inhibitionCell synapsesON pathwayGlycine receptorsLight decrementsVisual pathwayElectrical synapsesMeclofenamic acidDaylight vision
2007
Cellular Basis for Contrast Gain Control over the Receptive Field Center of Mammalian Retinal Ganglion Cells
Beaudoin DL, Borghuis BG, Demb JB. Cellular Basis for Contrast Gain Control over the Receptive Field Center of Mammalian Retinal Ganglion Cells. Journal Of Neuroscience 2007, 27: 2636-2645. PMID: 17344401, PMCID: PMC6672510, DOI: 10.1523/jneurosci.4610-06.2007.Peer-Reviewed Original ResearchConceptsReceptive field centerPresynaptic bipolar cellsGanglion cellsBipolar cellsAmacrine cellsGanglion cell receptive field centersContrast gain controlMammalian retinal ganglion cellsMammalian ganglion cellsRetinal ganglion cellsWhole-cell recordingsInhibitory neurotransmitter receptorsField centerVoltage-clamp conditionsInhibitory interneuronsSynaptic releaseNeurotransmitter receptorsCenter responseExtracellular recordingsPharmacological conditionsAdequate stimulationInhibitory conductanceCalcium bufferingSubthreshold responsesMembrane currents
2006
Making selective 'cone-ections'
Demb JB. Making selective 'cone-ections'. Nature Neuroscience 2006, 9: 595-596. PMID: 16639404, DOI: 10.1038/nn0506-595.Commentaries, Editorials and LettersPresynaptic Mechanism for Slow Contrast Adaptation in Mammalian Retinal Ganglion Cells
Manookin MB, Demb JB. Presynaptic Mechanism for Slow Contrast Adaptation in Mammalian Retinal Ganglion Cells. Neuron 2006, 50: 453-464. PMID: 16675399, DOI: 10.1016/j.neuron.2006.03.039.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAdaptation, PhysiologicalAnimalsCalcium ChannelsContrast SensitivityGlutamic AcidGuinea PigsOrgan Culture TechniquesPhotic StimulationPresynaptic TerminalsReaction TimeReceptors, GABAReceptors, GlycineReceptors, Metabotropic GlutamateRetinal Bipolar CellsRetinal Ganglion CellsSynaptic TransmissionConceptsPresynaptic bipolar cellsRetinal ganglion cellsGanglion cellsBipolar cellsMammalian retinal ganglion cellsSlow contrast adaptationReduced glutamate releaseIntrinsic cellular propertiesIntrinsic cellular mechanismsCortical adaptationGlutamate releasePresynaptic mechanismsGlycine receptorsVisual neuronsAfterhyperpolarizationExcitatory conductancePotassium conductanceContrast adaptationCellular mechanismsCortical cellsNeuronsCellsCellular propertiesMGluRsSpatial stimuli
2001
Bipolar Cells Contribute to Nonlinear Spatial Summation in the Brisk-Transient (Y) Ganglion Cell in Mammalian Retina
Demb J, Zaghloul K, Haarsma L, Sterling P. Bipolar Cells Contribute to Nonlinear Spatial Summation in the Brisk-Transient (Y) Ganglion Cell in Mammalian Retina. Journal Of Neuroscience 2001, 21: 7447-7454. PMID: 11567034, PMCID: PMC6762908, DOI: 10.1523/jneurosci.21-19-07447.2001.Peer-Reviewed Original ResearchConceptsBipolar cellsGanglion cellsBrisk-transient ganglion cellsReceptive fieldsExcitatory postsynaptic potentialsGuinea pig retinaNonlinear spatial summationNonlinear receptive fieldsAmacrine cellsPostsynaptic potentialsPig retinaExcitatory mechanismsMammalian retinaY cellsLinear receptive fieldsSpatial summationBipolar inputRetinaCellsPeripheral componentsCentral componentAcetylcholineTetrodotoxinGABA
1999
Functional Circuitry of the Retinal Ganglion Cell's Nonlinear Receptive Field
Demb J, Haarsma L, Freed M, Sterling P. Functional Circuitry of the Retinal Ganglion Cell's Nonlinear Receptive Field. Journal Of Neuroscience 1999, 19: 9756-9767. PMID: 10559385, PMCID: PMC6782950, DOI: 10.1523/jneurosci.19-22-09756.1999.Peer-Reviewed Original ResearchConceptsGanglion cellsDendritic fieldsContrast-reversing gratingsNonlinear receptive fieldsOFF ganglion cellsReceptive fieldsCell dendritic fieldsRetinal ganglion cellsGuinea pig retinaBipolar cell responsesReceptive field mechanismsSurround responsesBipolar cellsPig retinaCell responsesFunctional circuitryL-APVisual stimuliMembrane potentialSingle classCellsResponseInterneuronsTetrodotoxinRetina