2022
The force required to remove tubulin from the microtubule lattice by pulling on its α-tubulin C-terminal tail
Kuo YW, Mahamdeh M, Tuna Y, Howard J. The force required to remove tubulin from the microtubule lattice by pulling on its α-tubulin C-terminal tail. Nature Communications 2022, 13: 3651. PMID: 35752623, PMCID: PMC9233703, DOI: 10.1038/s41467-022-31069-x.Peer-Reviewed Original Research
2016
A force-generating machinery maintains the spindle at the cell center during mitosis
Garzon-Coral C, Fantana HA, Howard J. A force-generating machinery maintains the spindle at the cell center during mitosis. Science 2016, 352: 1124-1127. PMID: 27230381, PMCID: PMC6535051, DOI: 10.1126/science.aad9745.Peer-Reviewed Original ResearchConceptsForce-generating machineryMitotic spindleAsymmetric divisionDaughter cellsCell centerIntact cellsMagnetic tweezersCorrect localizationNumber of microtubulesMachinerySpindleForce generatorCellsCaenorhabditisAnaphaseLocalizationAccurate partitionsMitosisEmbryosMicrotubulesCytoplasmMetaphasePrecise localizationSmall cellsDivision
2006
Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner
Varga V, Helenius J, Tanaka K, Hyman AA, Tanaka TU, Howard J. Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner. Nature Cell Biology 2006, 8: 957-962. PMID: 16906145, DOI: 10.1038/ncb1462.Peer-Reviewed Original ResearchConceptsKinesin-8 proteinsLength-dependent depolymerizationYeast kinesin-8Length-dependent mannerMetazoan cellsMicrotubule cytoskeletonMitotic spindleDisassemble microtubulesSpindle lengthKinesin-8Kinesin-13Kip3pPlus endsMicrotubulesConstituent microtubulesLong microtubulesNew mechanismCytoskeletonFungiKinesinProteinSpindleCellsThe depolymerizing kinesin MCAK uses lattice diffusion to rapidly target microtubule ends
Helenius J, Brouhard G, Kalaidzidis Y, Diez S, Howard J. The depolymerizing kinesin MCAK uses lattice diffusion to rapidly target microtubule ends. Nature 2006, 441: 115-119. PMID: 16672973, DOI: 10.1038/nature04736.Peer-Reviewed Original Research
1994
The force exerted by a single kinesin molecule against a viscous load
Hunt AJ, Gittes F, Howard J. The force exerted by a single kinesin molecule against a viscous load. Biophysical Journal 1994, 67: 766-781. PMID: 7948690, PMCID: PMC1225420, DOI: 10.1016/s0006-3495(94)80537-5.Peer-Reviewed Original Research