1988
Subsets of GABAergic neurons in dissociated cell cultures of neonatal rat cerebral cortex show co-localization with specific modulator peptides.
Alho H, Ferrarese C, Vicini S, Vaccarino F. Subsets of GABAergic neurons in dissociated cell cultures of neonatal rat cerebral cortex show co-localization with specific modulator peptides. Brain Research 1988, 467: 193-204. PMID: 3378169, DOI: 10.1016/0165-3806(88)90023-5.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornCarrier ProteinsCells, CulturedCerebral CortexDiazepam Binding InhibitorElectrophysiologyGABA Plasma Membrane Transport ProteinsGamma-Aminobutyric AcidGlutamate DecarboxylaseMembrane ProteinsMembrane Transport ProteinsNerve Tissue ProteinsNeuronsNeuropeptidesOrganic Anion TransportersPeptide FragmentsRatsConceptsGlutamic acid decarboxylaseNeuropeptide YGABAergic propertiesCerebral cortexGAD immunoreactivityGABA-modulinNeonatal cortical neuronsPrimary culturesInhibitory synaptic currentsDouble-labeling studiesBenzodiazepine recognition sitesGABA receptor modulatorsUseful experimental modelDays of cultureNeuroregulatory peptideGABAergic neuronsGABAergic neurotransmissionSpontaneous excitatoryNeonatal ratsCortical neuronsReceptor modulatorsLower incidenceTyrosine hydroxylaseSynaptic currentsCultured neurons
1986
Modulation of gamma-aminobutyric acid-mediated inhibitory synaptic currents in dissociated cortical cell cultures.
Vicini S, Alho H, Costa E, Mienville JM, Santi MR, Vaccarino FM. Modulation of gamma-aminobutyric acid-mediated inhibitory synaptic currents in dissociated cortical cell cultures. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 9269-9273. PMID: 3097650, PMCID: PMC387117, DOI: 10.1073/pnas.83.23.9269.Peer-Reviewed Original ResearchConceptsInhibitory synaptic currentsSynaptic currentsRo 15Whole-cell patch-clamp techniqueActions of flunitrazepamGamma-aminobutyric acid receptorsNeonatal rat cortexCortical cell culturesPatch-clamp techniqueImidazobenzodiazepine Ro 15High-affinity ligandsRat cortexSynaptic transmissionPostsynaptic cellElectrical stimulationEndogenous ligandAcid receptorsImmunocytochemical stainingPrimary culturesNeuronsFlunitrazepamCell culturesIntrinsic activityDerivative methylAllosteric regulatory site