2024
Antagonism between neuropeptides and monoamines in a distributed circuit for pathogen avoidance
Marquina-Solis J, Feng L, Vandewyer E, Beets I, Hawk J, Colón-Ramos D, Yu J, Fox B, Schroeder F, Bargmann C. Antagonism between neuropeptides and monoamines in a distributed circuit for pathogen avoidance. Cell Reports 2024, 43: 114042. PMID: 38573858, PMCID: PMC11063628, DOI: 10.1016/j.celrep.2024.114042.Peer-Reviewed Original ResearchConceptsFLP-1 neuropeptidesBacterium Pseudomonas aeruginosa PA14Pathogen avoidanceGrowth factor BNematode Caenorhabditis elegansC. elegansCaenorhabditis elegansAvoidance signalPathogen infectionFlp-1NeuropeptidePathogensNeuronsMultiple mechanismsMultiple neuronsInfectionNeuromodulationPromote recoveryFactor BAvoidance behaviorAVKPA14Local and dynamic regulation of neuronal glycolysis in vivo
Wolfe A, Koberstein J, Smith C, Stewart M, Gonzalez I, Hammarlund M, Hyman A, Stork P, Goodman R, Colón-Ramos D. Local and dynamic regulation of neuronal glycolysis in vivo. Proceedings Of The National Academy Of Sciences Of The United States Of America 2024, 121: e2314699121. PMID: 38198527, PMCID: PMC10801914, DOI: 10.1073/pnas.2314699121.Peer-Reviewed Original ResearchConceptsGlycolytic stateEnergy stressEnergy metabolismConditions of energy stressDynamic regulationNeuronal functionIndividual cell typesMitochondrial localizationGenetic analysisSubcellular regionsRegulatory enzymeCell-autonomousNeuronal identityGlycolysisCell typesMetabolic stateImaging dynamic changesMetabolismLiving organismsIn vivoCellsEnergy landscapeIndividual neuronsEnzymeDynamic changes
2015
Untwisting the Caenorhabditis elegans embryo
Christensen RP, Bokinsky A, Santella A, Wu Y, Marquina-Solis J, Guo M, Kovacevic I, Kumar A, Winter PW, Tashakkori N, McCreedy E, Liu H, McAuliffe M, Mohler W, Colón-Ramos D, Bao Z, Shroff H. Untwisting the Caenorhabditis elegans embryo. ELife 2015, 4: e10070. PMID: 26633880, PMCID: PMC4764590, DOI: 10.7554/elife.10070.Peer-Reviewed Original ResearchConceptsCaenorhabditis elegansLate embryogenesisSeam cell nucleiSingle cell developmentNematode Caenorhabditis elegansEmbryonic nervous systemSystems-level viewWorm embryoInaccessible periodDevelopmental eventsCell developmentEmbryogenesisCell nucleiPositional informationElegansEmbryosLive embryosNeurodevelopmental eventsEmbryo movementAnnotation softwareCellsNervous systemEnough neuronsDetailed positional informationNeurons
2013
Synapse Location during Growth Depends on Glia Location
Shao Z, Watanabe S, Christensen R, Jorgensen EM, Colón-Ramos DA. Synapse Location during Growth Depends on Glia Location. Cell 2013, 154: 337-350. PMID: 23870123, PMCID: PMC3808971, DOI: 10.1016/j.cell.2013.06.028.Peer-Reviewed Original ResearchSerotonergic Neurosecretory Synapse Targeting Is Controlled by Netrin-Releasing Guidepost Neurons in Caenorhabditis elegans
Nelson JC, Colón-Ramos DA. Serotonergic Neurosecretory Synapse Targeting Is Controlled by Netrin-Releasing Guidepost Neurons in Caenorhabditis elegans. Journal Of Neuroscience 2013, 33: 1366-1376. PMID: 23345213, PMCID: PMC3584569, DOI: 10.1523/jneurosci.3471-12.2012.Peer-Reviewed Original ResearchConceptsUNC-34/EnabledReceptor UNC-40/DCCUNC-6/NetrinUNC-40/DCCNetrin receptor UNC-40/DCCCaenorhabditis elegansUNC-40NSM neuronsSynapse targetingMolecular linkMolecular mechanismsNovel mechanismSpatial specificitySite targetsRelease sitesNetrinPostsynaptic partnersSite formationRing neuronsSpecific regionsTargetingCaenorhabditisElegansLamellipodinGuidepost neurons
2012
Synaptic vesicle clustering requires a distinct MIG-10/Lamellipodin isoform and ABI-1 downstream from Netrin
Stavoe AK, Nelson JC, Martínez-Velázquez LA, Klein M, Samuel AD, Colón-Ramos DA. Synaptic vesicle clustering requires a distinct MIG-10/Lamellipodin isoform and ABI-1 downstream from Netrin. Genes & Development 2012, 26: 2206-2221. PMID: 23028145, PMCID: PMC3465741, DOI: 10.1101/gad.193409.112.Peer-Reviewed Original ResearchConceptsN-terminal domainActin cytoskeletonUnique N-terminal domainNeurodevelopmental programsSpecific subcellular domainsDistinct subcellular compartmentsN-terminal motifSynaptic vesicle clusteringIndividual cellular responsesNetrin signalsThermotaxis behaviorSubcellular compartmentsVesicle clusteringSubcellular domainsCellular responsesSynaptic vesiclesNetrinProtein 1IsoformsPresynaptic regionCytoskeletonMotifPresynaptic sitesVesiclesNeurodevelopmental responseNetrin instructs synaptic vesicle clustering through Rac GTPase, MIG-10, and the actin cytoskeleton
Stavoe AK, Colón-Ramos DA. Netrin instructs synaptic vesicle clustering through Rac GTPase, MIG-10, and the actin cytoskeleton. Journal Of Cell Biology 2012, 197: 75-88. PMID: 22451697, PMCID: PMC3317799, DOI: 10.1083/jcb.201110127.Peer-Reviewed Original ResearchConceptsActive zone proteinsActin cytoskeletonUNC-40Synaptic vesiclesSynapse assemblyVesicle clusteringCED-10/Rac1CED-5/DOCK180Receptor UNC-40/DCCPresynaptic regionUNC-40/DCCNetrin receptor UNC-40/DCCZone proteinSynaptic vesicle clusteringSeparate pathways downstreamRole of netrinsCED-5MIG-10Subcellular localizationRac GTPasePathways downstreamCytoskeletonCell migrationNetrinVesicles
2011
A conserved PTEN/FOXO pathway regulates neuronal morphology during C. elegans development
Christensen R, de la Torre-Ubieta L, Bonni A, Colón-Ramos DA. A conserved PTEN/FOXO pathway regulates neuronal morphology during C. elegans development. Development 2011, 138: 5257-5267. PMID: 22069193, PMCID: PMC3210501, DOI: 10.1242/dev.069062.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceAnimalsAnimals, Genetically ModifiedCaenorhabditis elegansCaenorhabditis elegans ProteinsForkhead Transcription FactorsImmunohistochemistryMicroscopy, FluorescenceNeuritesNeurogenesisPhosphatidylinositol 3-KinaseProtein IsoformsRNA InterferenceRNA, Small InterferingSignal TransductionTranscription FactorsTransgenesConceptsDAF-16/FOXOPI3KDAF-18/PTENC. elegans developmentSignal transduction cascadeNeurite outgrowthMajor negative regulatorPI3K pathway activityDaf-18Caenorhabditis elegansDAF-16Transduction cascadeFOXO pathwayNegative regulatorNeuronal cell morphologyNovel roleFOXOMammalian neuronsCorrect developmentPathway activityF isoformsNovel mechanismCell morphologyPathwayNeuronal morphology
2007
Glia Promote Local Synaptogenesis Through UNC-6 (Netrin) Signaling in C. elegans
Colón-Ramos D, Margeta MA, Shen K. Glia Promote Local Synaptogenesis Through UNC-6 (Netrin) Signaling in C. elegans. Science 2007, 318: 103-106. PMID: 17916735, PMCID: PMC2741089, DOI: 10.1126/science.1143762.Peer-Reviewed Original Research